CN-224212646-U - Single sperm capturing device based on micromanipulation needle and sperm cell PCR tube

Abstract

The utility model relates to a single sperm capturing device based on a micromanipulation needle and a sperm cell PCR tube, and belongs to the technical field of assisted reproduction and single cell operation. The device comprises a micro-operation module, a PCR tube mechanism and a composite operation dish, wherein the micro-operation module integrates a micro-operation needle and a micro-operation system to achieve single sperm grabbing and three-dimensional accurate positioning, the PCR tube mechanism forms a sperm pool through 2 mu LPBS liquid drops on the tube wall, 3 mu LPBS buffer solution is preset at the bottom to construct a gradient liquid film to optimize sperm positioning, and the composite operation dish adopts a 'one-pool double-area' structure to achieve full-flow integration of sperm braking, cleaning and transferring. The device eliminates the traditional liquid drop transfer step by directly discharging sperm into a pool through the micromanipulation needle, effectively shortens the single operation time, effectively improves the capturing success rate, and provides a high-efficiency and stable technical platform for single sperm genome analysis.

Inventors

• YUAN ZHENYA
• CAI QIAN
• YUAN MU
• BAI MINGZHU
• ZHANG XUE
• SONG XUEMEI
• Yan Weiqiao

Assignees

• 徐州市妇幼保健院

Dates

Publication Date

20260508

Application Date

20250529

Claims (4)

1. 1. A single sperm capturing device based on a micromanipulation needle and sperm cell PCR tube, comprising: A micromanipulation module (1) comprising the micromanipulation needle (11) and a micromanipulation system (12); The PCR tube mechanism (2), the tube wall of the PCR tube mechanism (2) is provided with a sperm pool (21) for accommodating sperms, and the bottom of the tube wall of the PCR tube mechanism (2) is provided with PBS buffer solution drops (22); the composite operation dish (3) is provided with a rectangular sperm swimming pool (31), a PVP braking area (32) and a PBS cleaning area (33).
2. 2. A single sperm cell as described in claim 1, wherein said sperm cell is comprised of 2 microliter drops of PBS, and wherein the volume of the PBS buffer drops is 3 microliter.
3. 3. A single sperm cell as described in claim 1, wherein said rectangular sperm cell (31) is provided in one number, and said PVP stopper region (32) and PBS wash region (33) are provided in two numbers, respectively.
4. 4. The single sperm capturing device based on the micro-manipulation needle and the sperm cell PCR tube according to claim 1, wherein the surfaces of the rectangular sperm swimming pool (31), the PVP braking area (32) and the PBS cleaning area (33) of the composite manipulation vessel (3) are subjected to hydrophobic treatment to form an annular hydrophobic boundary, and the center of each area is a hydrophilic contact surface.

Description

Single sperm capturing device based on micromanipulation needle and sperm cell PCR tube Technical Field The utility model relates to the technical field of auxiliary reproduction and single cell operation, in particular to a single sperm capturing device based on a micromanipulation needle and a sperm cell PCR tube. Background The gene sequencing, transcriptome sequencing and the like of single sperms are of great significance to male genetic disease research. Effective gripping and preservation of individual sperm is a prerequisite for intensive research. Currently, the prior art is to collect individual sperm cells under a microscope using a mouth pipette or micropipette. The specific method is to brake single sperms by using a micro-operation system, grasp the single sperms by using a micro-operation needle, place the single sperms in PBS liquid drops, aspirate the single sperms under a microscope by using a mouth pipette or a micropipette, and place the single sperms in a PCR tube containing 5 microliters of PBS. However, the oral pipette may cause contamination of bacteria, fungi, viruses, etc. or foreign DNA, and the method has high technical requirements for operators, and is easy to lose in the process of transferring to the PCR tube because of thicker PBS liquid level in the PCR tube. Furthermore, this process may result in PCR tubes being injected with excess PBS liquid due to oral pipettes or manual handling of micropipettes, which may negatively interfere with subsequent single sperm amplification systems and their success rates. Disclosure of utility model The utility model provides a single sperm capturing device based on a micromanipulation needle and a sperm cell PCR tube, aiming at solving the problems in the prior art. In order to solve the technical problems, the utility model is realized by the following technical scheme that the single sperm capturing device based on the micromanipulation needle and the sperm cell PCR tube comprises: The micromanipulation module comprises the micromanipulation needle and a micromanipulation system; The wall of the PCR tube mechanism is provided with a sperm pool for accommodating sperms, and the bottom of the wall of the PCR tube mechanism is provided with PBS buffer solution drops; The composite operation dish is provided with a rectangular sperm swimming pool, a PVP braking area and a PBS cleaning area. After single sperm is grasped by using a micromanipulation needle, 2 microliter of PBS is pumped from a PCR tube by using a micropipette and is dripped into the position of a sperm cell. The PCR tube is placed horizontally, a single sperm is discharged into a sperm pool on the wall of the PCR tube by using a micromanipulation needle under a microscope, and the sperm is stored after centrifugation. The device is simple to operate, high in efficiency and capable of being effectively used for follow-up research. Preferably, the sperm cell is made up of 2 microliter drops of PBS, the volume of which is provided with 3 microliters. Preferably, the number of the rectangular sperm swimming pools is set to be one, and the PVP braking area and the PBS cleaning area are respectively set to be two. Preferably, the surfaces of the rectangular sperm swimming pool, the PVP braking area and the PBS cleaning area of the composite operation dish are subjected to hydrophobic treatment to form annular hydrophobic boundaries, and the centers of the areas are hydrophilic contact surfaces. The beneficial effects of the utility model are as follows: The utility model breaks through the technical bottleneck that the traditional single sperm capturing needs multiple device switching by the design of an integrated operation system of the micro-operation module and the PCR tube mechanism. The method is characterized by simplifying an operation path, eliminating redundant steps such as liquid drop transfer, microscope repositioning and the like in the traditional method by utilizing the 'grabbing-braking-discharging' integrated action of a micro-operation needle, shortening the single operation time to 1+/-0.2 minutes (compared with the traditional method for 1.5+/-0.2 minutes), quantitatively improving the success rate, and effectively improving the single sperm capturing success rate by the synergistic effect of a '1+2+2' functional partition (1 swimming pool+2 braking area+2 cleaning area) of a composite operation dish and a PCR tube wall sperm pool. Drawings FIG. 1 is a schematic view of the overall structure of the composite operating dish of the present utility model. FIG. 2 is a schematic diagram showing the operation structure of a PCR tube mechanism according to the present utility model. Fig. 3 is a schematic representation of clinical validation experimental data of the present utility model. In the figures 1 to 3, 1, a micro-operation module, 11, a micro-operation needle, 12, a micro-operation system, 2, a PCR tube mechanism, 21, a sperm cell, 22, PBS buffer solution