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CN-224212658-U - Pathogen targeted sequencing sample preparation system

CN224212658UCN 224212658 UCN224212658 UCN 224212658UCN-224212658-U

Abstract

The utility model relates to the field of medical instruments and discloses a pathogen targeted sequencing sample preparation system, which comprises a frame assembly, a mechanical arm assembly, a gas circulation assembly and a shell assembly, wherein a working platform is arranged in the frame assembly, the working platform is provided with a sample loading needle box installation module, a sample plate installation module, a normal-temperature reagent plate installation module, a refrigeration reagent plate installation module, a fluorescence detection module, a magnetic suction module, an oscillation incubation module, a PCR module, a waste needle waste liquid receiving structure and a cover plate receiving module, the mechanical arm assembly comprises a pipetting mechanical arm, a grabbing plate pipetting mechanical arm and a first driving assembly, the gas circulation assembly comprises an air inlet assembly and an air outlet assembly which are arranged in the frame assembly and/or the shell assembly, the air outlet end of the air inlet assembly corresponds to the position of the PCR module, and the air inlet end of the air outlet assembly corresponds to the position of the PCR module. The pathogen targeted sequencing sample preparation system can prevent hot air generated by the operation of the PCR module from being mixed in the system.

Inventors

  • CAI XIAOXIANG
  • XUE JIANAN

Assignees

  • 长沙迈迪克智能科技有限公司

Dates

Publication Date
20260508
Application Date
20250401

Claims (8)

  1. 1. A pathogen-targeted sequencing sample preparation system, wherein the pathogen-targeted sequencing sample preparation system comprises: The device comprises a frame assembly (1), wherein a working platform (11) is arranged in the frame assembly (1), and the working platform (11) is provided with a sample loading needle box installation module (111), a sample plate installation module (112), a normal temperature reagent plate installation module (113), a refrigerated reagent plate installation module (114), a fluorescence detection module (115), a magnetic attraction module (116), an oscillation incubation module (117), a PCR module (118), a waste needle waste liquid receiving structure (119) and a cover plate receiving module (120) for receiving and storing a PCR cover plate; The mechanical arm assembly (2), the mechanical arm assembly (2) is arranged in the frame assembly (1) and is positioned above the working platform (11), and the mechanical arm assembly (2) comprises a liquid transferring mechanical arm (21) for transferring a liquid sample by installing a sample adding needle, a grabbing plate transferring mechanical arm (22) for clamping and transferring a deep hole plate or a PCR cover plate, and a first driving assembly (23) for driving the liquid transferring mechanical arm (21) and the grabbing plate transferring mechanical arm (22) to move along a first direction (A); A housing assembly (3), said housing assembly (3) being mounted to said frame assembly (1), and The gas circulation assembly (4), the gas circulation assembly (4) comprises an air inlet assembly (41) and an air outlet assembly (42) which are arranged on the frame assembly (1) and/or the shell assembly (3), the air outlet end of the air inlet assembly (41) corresponds to the position of the PCR module (118), and the air inlet end of the air outlet assembly (42) corresponds to the position of the PCR module (118).
  2. 2. Pathogen targeted sequencing sample preparation system according to claim 1, characterized in that the interior of the frame assembly (1) is provided with a platform back plate (12) perpendicular to the working platform (11), the air inlet assembly (41) is mounted to the platform back plate (12), the platform back plate (12) is provided with a grid air inlet opening (121) corresponding to the air inlet assembly (41), and the rear part of the housing assembly (3) is provided with a first avoidance opening (31) corresponding to the air inlet assembly (41).
  3. 3. Pathogen targeted sequencing sample preparation system according to claim 2, characterized in that the air intake assembly (41) comprises: A first shell (411), wherein an air inlet channel is arranged in the first shell (411), and the first shell (411) is mounted on the platform backboard (12); a first filter element mounted inside the first housing (411) and located in the intake passage, and A first fan assembly mounted inside the first housing (411) and located in the intake passage.
  4. 4. Pathogen targeted sequencing sample preparation system according to claim 1 or 2, characterized in that the exhaust assembly (42) is mounted on top of the housing assembly (3), the top of the frame assembly (1) being provided with a second avoidance opening (13) corresponding to the exhaust assembly (42).
  5. 5. The pathogen targeted sequencing sample preparation system of claim 4, wherein the exhaust assembly (42) comprises: A second housing (421), an exhaust passage being provided inside the second housing (421), the second housing (421) being mounted on top of the housing assembly (3); A second filter element mounted inside the second case (421) and located in the exhaust passage, and And a second fan assembly installed inside the second housing (421) and located in the exhaust passage.
  6. 6. The pathogen targeted sequencing sample preparation system of claim 1 or 2, wherein the pipetting robot arm (21) comprises a first robot arm frame assembly (211) movably mounted to the frame assembly (1) in the first direction (a), a pipetting module (212) movably mounted to the first robot arm frame assembly (211) in a second direction (B), and a pipetting module drive assembly (213) for driving the pipetting module (212) to move in the second direction (B), the pipetting module (212) being adapted to pipetting liquid samples by mounting a sample application needle and being extendable and contractible in a third direction (C), the first direction (a), the second direction (B) and the third direction (C) being mutually perpendicular to each other.
  7. 7. The pathogen targeted sequencing sample preparation system of claim 6, wherein the gripper plate shift robotic arm (22) comprises a second robotic arm frame assembly (221) movably mounted to the frame assembly (1) along the first direction (a), a gripper module (222) movably mounted to the second robotic arm frame assembly (221) along the second direction (B), and a gripper module drive assembly (223) for driving the gripper module (222) to move along the second direction (B), the gripper module (222) being for gripping a deep well plate or PCR cover plate and being capable of being elongated and shortened along the third direction (C).
  8. 8. The pathogen targeted sequencing sample preparation system of claim 7, wherein the first and second robotic arm frame assemblies (211, 221) are securely connected to each other.

Description

Pathogen targeted sequencing sample preparation system Technical Field The utility model relates to the field of medical instruments, in particular to a pathogen targeted sequencing sample preparation system. Background Pathogen targeted sequencing (tNGS) is a new generation sequencing technology based on the combination of super-multiplex PCR amplification (or targeted capture) and high-throughput sequencing technologies, and can directly enrich dozens to hundreds of known pathogen microorganisms and virulence and/or drug resistance genes thereof in clinical samples without relying on traditional microorganism culture, then perform high-throughput sequencing, then compare with a database for analysis, and judge the types of the pathogen microorganisms contained in the samples according to the compared sequence information. The pathogenic targeting sequencing can rapidly and objectively detect more pathogenic microorganisms in clinical samples, and provides basis for clinical diagnosis. Detection of low concentrations of pathogenic microorganisms, in particular virulence and/or drug resistance genes thereof, is particularly advantageous. However, the current application scenario is to use a general gene sequencing sample preparation system to prepare a pathogen targeted sequencing sample, the current gene sequencing sample preparation system is usually only provided with an air inlet component at the top, and a large amount of hot air generated by the operation of a PCR module can be mixed in the system in the process of preparing the pathogen targeted sequencing sample, so that cross contamination risk is brought to an experimental sample. In view of the foregoing, there is a need in the art for a pathogen-targeted sequencing sample preparation system that can prevent hot air generated by the operation of a PCR module from being misdirected within the system. Disclosure of Invention The utility model aims to provide a pathogen targeted sequencing sample preparation system capable of preventing hot air generated by the operation of a PCR module from randomly channeling in the system. In order to achieve the aim, the pathogen targeted sequencing sample preparation system comprises a frame assembly, a mechanical arm assembly, a gas circulation assembly and a PCR (polymerase chain reaction) assembly, wherein a working platform is arranged in the frame assembly, the working platform is provided with a sample loading needle box installation module, a sample plate installation module, a normal-temperature reagent plate installation module, a cold-storage reagent plate installation module, a fluorescence detection module, a magnetic suction module, an oscillation incubation module, a PCR module, a waste needle waste liquid receiving structure and a cover plate receiving module for receiving and storing a PCR cover plate, the mechanical arm assembly is arranged in the frame assembly and is positioned above the working platform, the mechanical arm assembly comprises a liquid transferring mechanical arm for transferring a liquid sample by installing a sample loading needle, a plate grabbing and plate transferring mechanical arm for clamping and transferring a deep pore plate or a PCR cover plate, and a first driving assembly for driving the liquid transferring mechanical arm and the plate grabbing and the plate transferring mechanical arm to move along a first direction, the housing assembly is arranged on the frame assembly, the housing assembly, the gas circulation assembly comprises a gas inlet end and a gas outlet end, and a gas circulation assembly are arranged on the housing assembly or the housing assembly, and the gas inlet end of the housing assembly corresponds to the position of the gas inlet end and the gas inlet end of the PCR assembly. Specifically, the inside of frame subassembly is provided with the perpendicular to work platform's platform backplate, the subassembly of admitting air install in the platform backplate, the platform backplate be provided with the grille inlet opening that the subassembly corresponds admitting air, the rear portion of shell subassembly be provided with the first opening of dodging that the subassembly corresponds admitting air. The air inlet assembly comprises a first shell, a first filter element and a first fan assembly, wherein an air inlet channel is formed in the first shell, the first shell is mounted on the platform backboard, the first filter element is mounted in the first shell and located in the air inlet channel, and the first fan assembly is mounted in the first shell and located in the air inlet channel. Specifically, the exhaust assembly is installed in the top of shell subassembly, the top of frame subassembly is provided with the second of corresponding with exhaust assembly dodges the opening. The exhaust assembly comprises a second shell, a second filter element and a second fan assembly, wherein an exhaust channel is arranged in the second shell, the