CN-224212659-U - AKK fungus high-efficient continuous culture device

Abstract

The utility model discloses an AKK (alkyl ketene dimer) bacteria efficient continuous culture device, which belongs to the technical field of anaerobic bacteria culture and comprises an anaerobic incubator and an anaerobic fermentation tank, wherein the anaerobic fermentation tank is positioned in the anaerobic incubator, a mother liquor tank and three culture medium preparation tanks are arranged beside the anaerobic fermentation tank, culture medium supply mechanisms are arranged on the mother liquor tank and the three culture medium preparation tanks and the anaerobic fermentation tank, the mother liquor tank and the three culture medium preparation tanks are communicated with the anaerobic fermentation tank through the culture medium supply mechanisms, a gas conveying mechanism is arranged at the top of the anaerobic fermentation tank and communicated with the anaerobic fermentation tank, and a discharge channel is arranged at the bottom of the anaerobic fermentation tank. According to the utility model, the culture conditions can be timely adjusted, so that each batch of AKK bacteria can grow under the same optimal conditions, the uniformity and stability of the product quality are effectively ensured, and the product quality is improved.

Inventors

• GAO YUTING
• SHI XINLI
• JIA RUILONG

Assignees

• 山西中医药大学

Dates

Publication Date

20260508

Application Date

20250515

Claims (10)

1. 1. The AKK bacteria efficient continuous culture device is characterized by comprising an anaerobic incubator (1) and an anaerobic fermentation tank (2), wherein the anaerobic fermentation tank (2) is positioned inside the anaerobic incubator (1), a mother liquor tank (3) and three culture medium preparation tanks (4) are arranged beside the anaerobic fermentation tank (2), culture medium supply mechanisms (5) are arranged on the mother liquor tank (3) and the three culture medium preparation tanks (4) and the anaerobic fermentation tank (2), the mother liquor tank (3) and the three culture medium preparation tanks (4) are communicated with the anaerobic fermentation tank (2) through the culture medium supply mechanisms (5), a gas conveying mechanism (6) is arranged at the top of the anaerobic fermentation tank (2), the gas conveying mechanism (6) is communicated with the anaerobic fermentation tank (2), and a discharge channel (7) is arranged at the bottom of the anaerobic fermentation tank (2).
2. 2. The AKK bacteria efficient continuous culture device as claimed in claim 1, wherein each culture medium supply mechanism (5) comprises a peristaltic pump (51), a liquid inlet pipeline (52) and a liquid outlet pipeline (53), the peristaltic pump (51) is connected to the side wall of the anaerobic fermentation tank (2), two ends of the liquid outlet pipeline (53) are respectively communicated with the peristaltic pump (51) and the anaerobic fermentation tank (2), and the liquid inlet pipeline (52) is respectively communicated with the peristaltic pump (51) and the anaerobic fermentation tank (2) or the mother liquor tank (3).
3. 3. The AKK bacteria high-efficiency continuous culture device as claimed in claim 2, wherein the liquid outlet pipeline (53) is provided with a flowmeter.
4. 4. The AKK bacteria efficient continuous culture device as claimed in claim 1, wherein a motor base (8) is arranged at the bottom of the anaerobic fermentation tank (2), a stirring motor (81) is arranged on the motor base (8), a stirring shaft (82) which is vertically arranged is arranged on a main shaft of the stirring motor (81), the stirring shaft (82) extends into the anaerobic fermentation tank (2), and a plurality of stirring rods (83) are arranged on the stirring shaft (82).
5. 5. The AKK bacteria high-efficiency continuous culture device as claimed in claim 1, wherein a pressure gauge, a PH probe and a biosensor analyzer are arranged in the anaerobic fermentation tank (2).
6. 6. The AKK bacteria high-efficiency continuous culture device according to claim 1, wherein a mass flowmeter is arranged in the gas conveying mechanism (6).
7. 7. The AKK bacteria high-efficiency continuous culture device as claimed in claim 1, wherein a gas exchange machine (11) and a circulating fan (12) are arranged on the side wall of the anaerobic incubator (1), an oxygen sensor and a carbon dioxide sensor are arranged on the inner side wall of the anaerobic incubator (1), and the gas exchange machine (11) and the circulating fan (12) are electrically connected with the oxygen sensor and the carbon dioxide sensor.
8. 8. The AKK bacteria high-efficiency continuous culture device as claimed in claim 1, wherein the discharge channel (7) is provided with a closed opening and closing door (71).
9. 9. The AKK bacteria high-efficiency continuous culture device as claimed in claim 1, wherein a sealing door (13) is arranged on the side wall of the anaerobic incubator (1).
10. 10. The AKK bacteria high-efficiency continuous culture device as claimed in claim 1, wherein the three culture medium preparation tanks (4) are liquid storage tanks filled with Chinese medicinal culture medium respectively.

Description

AKK fungus high-efficient continuous culture device Technical Field The utility model belongs to the technical field of anaerobic bacteria culture, and particularly relates to an AKK (alkyl ketene dimer) bacteria efficient continuous culture device. Background AKK bacteria (Akkermansiamuciniphila) as important probiotics in intestinal tracts have great potential in regulating intestinal flora balance, enhancing host immune function, improving metabolic diseases and the like, and related products have wide application prospects in the fields of biological medicines, functional foods and the like. However, the current industrial culture of AKK bacteria faces a number of technical bottlenecks. The traditional AKK bacteria are cultivated in a batch cultivation mode, and the mode has obvious defects. On the one hand, in the batch culture process, along with the consumption of nutrient substances and the accumulation of metabolites, the culture environment can be changed drastically, and the optimal growth condition of AKK bacteria is difficult to maintain for a long time, so that the cell growth density is low, the production efficiency is low, and the growing market demands cannot be met. On the other hand, batch culture is difficult to realize precise control of the culture process, and uniformity and stability of product quality are difficult to ensure. In the process of preparing and conveying the culture medium, the conventional equipment often lacks accurate proportioning control capability. AKK bacteria grow very sensitive to the proportion of nutrient components, and if the proportion of carbon source, nitrogen source, special growth factors and other components is improper, the growth metabolism and activity of AKK bacteria can be seriously affected. The traditional equipment can not dynamically and accurately supply various nutrient components according to the growth characteristics of AKK bacteria, so that the nutrition utilization efficiency is low, and the culture cost is high. In addition, AKK bacteria are strict anaerobes, and have strict requirements on the oxygen content in the culture environment. The common anaerobic culture equipment has the defects in the aspects of gas replacement and environmental maintenance, and is difficult to ensure an oxygen-free environment stable for a long time, and tiny oxygen infiltration can inhibit the growth of AKK bacteria and even lead to bacterial death. Meanwhile, parameters such as temperature, pH value and the like in the equipment are difficult to keep stable, and the culture effect of AKK bacteria is further affected. Disclosure of utility model The embodiment of the utility model provides an AKK (alkyl ketene dimer) bacteria efficient continuous culture device, which aims to solve the problems in the prior art. The embodiment of the utility model adopts the following technical scheme that the AKK bacteria high-efficiency continuous culture device comprises an anaerobic incubator and an anaerobic fermentation tank, wherein the anaerobic fermentation tank is positioned in the anaerobic incubator, a mother liquor tank and three culture medium preparation tanks are arranged beside the anaerobic fermentation tank, culture medium supply mechanisms are arranged on the mother liquor tank, the three culture medium preparation tanks and the anaerobic fermentation tank, the mother liquor tank and the three culture medium preparation tanks are communicated with the anaerobic fermentation tank through the culture medium supply mechanisms, a gas conveying mechanism is arranged at the top of the anaerobic fermentation tank and communicated with the anaerobic fermentation tank, and a discharge channel is arranged at the bottom of the anaerobic fermentation tank. According to a further technical scheme, each culture medium supply mechanism comprises a peristaltic pump, a liquid inlet pipeline and a liquid outlet pipeline, the peristaltic pump is connected to the side wall of the anaerobic fermentation tank, two ends of the liquid outlet pipeline are respectively communicated with the peristaltic pump and the anaerobic fermentation tank, and the liquid inlet pipeline is respectively communicated with the peristaltic pump and the anaerobic fermentation tank or the mother liquor tank. According to a further technical scheme, a flowmeter is arranged on the liquid outlet pipeline. Further technical scheme, anaerobic fermentation tank's bottom is equipped with the motor cabinet, be equipped with agitator motor on the motor cabinet, be equipped with the (mixing) shaft of vertical setting on agitator motor's the main shaft, the (mixing) shaft extends to inside the anaerobic fermentation tank, be equipped with a plurality of puddlers on the (mixing) shaft. According to a further technical scheme, a pressure gauge, a PH probe and a biosensor analyzer are arranged in the anaerobic fermentation tank. According to a further technical scheme, a mass flowmeter is arranged in the gas