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CN-224212666-U - Cell co-culture device

CN224212666UCN 224212666 UCN224212666 UCN 224212666UCN-224212666-U

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell co-culture device which comprises a box body with an inner cavity, at least one partition plate and at least one filter plate, wherein at least one socket is arranged in the box body, the partition plate and the filter plate can be respectively and detachably connected with the socket to divide the inner cavity into at least two chambers, the filter plate is provided with a cell filter membrane, each chamber is used for culturing cells with different sizes, and the pore size of the cell filter membrane is between the sizes of cells cultured in two adjacent chambers. The inner cavity is divided into different chambers through the partition plates so as to be used for culturing different cells, and the inner cavity is divided through the filter plates, so that mixed culture solution is filtered through the filter plates, and different cells are separated so as to be convenient for analysis. The whole culture and separation process is completed in the cell co-culture device, so that the use is convenient and the efficiency is higher.

Inventors

  • TANG YING
  • Cui tianqi
  • ZHOU RUISHENG
  • GAO YONG
  • TANG XI

Assignees

  • 广州中医药大学(广州中医药研究院)

Dates

Publication Date
20260508
Application Date
20250306

Claims (7)

  1. 1. The cell co-culture device is characterized by comprising a box body (1) with an inner cavity, at least one partition plate (2) and at least one filter plate (3), wherein the box body (1) is provided with at least one socket (4), the partition plate (2) and the filter plate (3) can be respectively detachably connected with the socket (4) to divide the inner cavity into at least two chambers (11), the filter plate (3) is provided with a cell filter membrane (31), each chamber (11) is used for culturing cells with different sizes, and the membrane pore size of the cell filter membrane (31) is between the cell sizes cultured by two adjacent chambers (11); The filter plate (3) further comprises a frame piece (32), the cell filter membrane (31) is arranged in the frame piece (32), a second sealing groove (33) is formed in the circumference of the filter plate (3), and a second sealing ring is arranged in the second sealing groove (33).
  2. 2. The cell co-culture apparatus according to claim 1, wherein the partition plate (2), the filter plate (3) and the insertion opening (4) are provided in plural, the inner cavity is partitioned into plural chambers (11) by the partition plate (2) or the filter plate (3), and the membrane pore sizes of the cell filtration membranes (31) are arranged in order.
  3. 3. A cell co-culture apparatus according to claim 1, wherein each of said chambers (11) is arranged in a vertical order.
  4. 4. The cell co-culture apparatus according to claim 3, wherein the socket (4) comprises a first groove body (41) and a second groove body (42), the first groove body (41) and the second groove body (42) are respectively arranged in the box body (1), the first groove body (41) is in sliding connection with the partition plate (2), the second groove body (42) is in sliding connection with the filter plate (3), and the first groove body (41) is positioned on the upper side of the second groove body (42).
  5. 5. A cell co-culture apparatus according to claim 1, wherein the side walls of each chamber (11) are provided with an inlet portion (12), respectively.
  6. 6. A cell co-culture apparatus according to claim 1, wherein the partition plate (2) and the filter plate (3) are connected to a first handle (22) and a second handle (34), respectively.
  7. 7. The cell co-culture apparatus according to claim 1, wherein the top and bottom of the housing (1) are each provided with a detachable end plate (6).

Description

Cell co-culture device Technical Field The utility model relates to the technical field of cell culture, in particular to a cell co-culture device. Background Cell co-cultivation refers to a technique in which two or more different types of cells are co-cultivated in the same cultivation system. The technology can simulate the interaction among different cells in the body and study the biological processes of signal transmission, metabolic influence, growth regulation and the like among the cells. In the case of co-culturing cells requiring direct contact, it is first necessary to separately culture different cells, then mix and further culture the cultured cells, and finally isolate and observe and analyze the changes produced by the cells. And after different cells are mixed and cultured, each cell is separated, the separation process is complicated, and the experimental efficiency is low. Disclosure of utility model The utility model provides a cell co-culture device which can effectively improve the cell separation efficiency and the experimental efficiency in order to solve the problem that the cell separation is inconvenient in the cell co-culture process in the prior art. In order to solve the technical problems, the cell co-culture device comprises a box body with an inner cavity, at least one partition plate and at least one filter plate, wherein the box body is provided with at least one socket, the partition plate and the filter plate can be detachably connected with the socket respectively to partition the inner cavity into at least two chambers, the filter plate is provided with cell filtration membranes, each chamber is used for culturing cells with different sizes, and the pore diameter of each cell filtration membrane is between the sizes of cells cultured in two adjacent chambers. In the technical scheme of the utility model, at the stage of preliminary cell culture, the inner cavity is divided into different chambers by the partition plate so as to be used for culturing different cells, then the partition plate is disassembled so as to mix different cells and culture solutions thereof, after mixed culture is finished, the inner cavity is divided by the filter plate, and the mixed culture solution is filtered by the filter plate, so that different cells are separated so as to be convenient for analysis. The whole culture and separation process is completed in the cell co-culture device, so that the use is convenient and the efficiency is higher. Further, the separation plate, the filter plate and the insertion opening are all provided with a plurality of holes, the inner cavity is divided into a plurality of chambers by the separation plate or the filter plate, and the membrane pore sizes of the cell filtration membranes are sequentially arranged. In the scheme, a plurality of kinds of cells with different sizes can be cultured through a plurality of chambers, and as the membrane pore sizes of the cell filtration membranes are sequentially arranged, the cell sizes in the chambers are sequentially arranged, and when the cells are separated, the mixed culture solution can be sequentially separated through the filtration plates to obtain the cells, so that the efficiency is higher. Further, each chamber is vertically and sequentially arranged. In the scheme, the chambers are vertically and sequentially arranged, cells are cultured in the chambers from top to bottom according to the order of the cell sizes from small to large in the stage of cell culture respectively, the cells cultured in the chambers fall to the bottommost layer after the separation plates are disassembled for mixed culture, and after the culture is completed, the filter plates are arranged in the sockets according to the cell size requirement, the box body is inverted, and the mixed culture solution sequentially passes through the filter plates to separate the cells. Further, the socket comprises a first groove body and a second groove body, the first groove body and the second groove body are respectively arranged in the box body, the first groove body is in sliding connection with the partition plate, the second groove body is in sliding connection with the filter plate, and the first groove body is located on the upper side of the second groove body. In the scheme, the partition plate and the filter plate are respectively connected in the first tank body and the second tank body in a sliding manner when the cells are cultured, and as the first tank body is positioned on the upper side of the second tank body, the bottom of each layer of chambers is separated by the partition plate and cannot fall from the filter plate when the cells are cultured respectively, the partition plate and the filter plate are outwards slid to enable the cells cultured in the chambers to fall into the bottommost chamber for mixed culture, the filter plate is inserted into and separated from the chambers in a sliding manner after the culture is c