Search

CN-224212667-U - Closed fluid infusion device for suspension cell culture

CN224212667UCN 224212667 UCN224212667 UCN 224212667UCN-224212667-U

Abstract

The application relates to the technical field of cell culture and discloses a closed liquid supplementing device for suspension cell culture, which comprises a liquid transferring device and a peristaltic pump, wherein the liquid transferring device comprises a liquid supplementing pipe, a first interface device and a second interface device, one end of the liquid supplementing pipe is connected to a culture medium bottle through the first interface device formed by a medical thread conversion cover, a one-way valve and a filter, the other end of the liquid supplementing pipe is connected to a culture bag through the second interface device to form a closed system, and the peristaltic pump is used for pumping liquid in the culture medium bottle into the culture bag at a uniform speed in a one-way mode, and the liquid supplementing pipe is placed in the peristaltic pump when the liquid supplementing device is used. The application realizes the airtight, accurate and efficient fluid infusion operation in the cell culture process.

Inventors

  • WU HAO
  • LIU HAO
  • JIANG LEI
  • HU YUAN
  • ZHAO YI

Assignees

  • 杭州华赛伯曼医学细胞生物有限公司

Dates

Publication Date
20260508
Application Date
20250328

Claims (10)

  1. 1. A closed fluid infusion device for suspension cell culture is characterized by comprising a fluid transfer device (1) and a peristaltic pump (2): The liquid transferring device (1) comprises a liquid supplementing pipe (101), a first interface device and a second interface device, wherein one end of the liquid supplementing pipe (101) is connected to a culture medium bottle (3) through the first interface device formed by a medical thread conversion cover (102), a one-way valve (103) and a filter (104), the other end of the liquid supplementing pipe (101) is connected to a culture bag (4) through the second interface device, and a closed system is formed among the culture medium bottle (3), the liquid transferring device (1) and the culture bag (4); The peristaltic pump (2) is used for pumping the liquid in the culture medium bottle (3) into the culture bag (4) at a uniform speed in a unidirectional way, and the liquid supplementing pipe (101) is placed in the peristaltic pump (2) when the peristaltic pump is used.
  2. 2. The device according to claim 1, wherein in the first interface device, the medical thread conversion cover (102) is matched with the outlet of the culture medium bottle (3) in size and sleeved at the outlet of the culture medium bottle (3), the cover cap of the medical thread conversion cover (102) is provided with two round holes, one end of the fluid infusion tube (101) and one end of the one-way valve (103) are respectively connected outside the cover cap through the two round holes, and the other end of the one-way valve (103) is connected with the filter (104); The one-way valve (103) is used for controlling the air to flow from the outside to the inside of the culture medium bottle (3), and the filter (104) is used for filtering impurities in the air.
  3. 3. The device according to claim 1, characterized in that said one-way valve (103) is constituted by a plurality of segments of different diameter tubes, one of which has inside a circular one-way flap; The filter (104) is formed by sequentially connecting a connecting pipe, a disc-shaped structure and an inlet circular pipe, wherein a filtering membrane is arranged in the disc-shaped structure; the one-way valve (103) is connected with a connecting pipe of the filter (104).
  4. 4. The device according to claim 1, further comprising a scale (5); and the scale (5) is used for weighing the mass of the culture bag (4) in real time in the process of replenishing liquid and monitoring the change of the mass of the liquid in the culture bag (4).
  5. 5. The device according to claim 4, further comprising a controller (6), said controller (6) being electrically connected to said scale (5) and said peristaltic pump (2) for receiving a quality signal from said scale (5) and sending a stop signal to stop operation of said peristaltic pump (2) when the quality of said culture bag (4) reaches a desired quality.
  6. 6. The device of claim 1, wherein the second interface means comprises a luer fitting (105), a PVC heat seal tube, or a sterile tube-taking machine.
  7. 7. The device according to claim 1, the peristaltic pump (2) having a function of controlling the flow.
  8. 8. The device according to claim 1, wherein the fluid replacement tube (101) comprises a silicone tube, a sterile polyvinyl chloride tube or a thermoplastic elastomer tube.
  9. 9. The device according to claim 1, characterized in that the liquid transfer device (1) further comprises a first water stop clamp (106), the culture bag (4) further comprising a second water stop clamp; The first water stop clamp (106) is used for controlling the circulation and stop of the liquid in the liquid supplementing pipe (101), and is clamped on the liquid supplementing pipe (101) when in use; The second water stop clamp is used for controlling the circulation and stop of liquid in the inlet connecting pipe (401) of the culture bag (4), and is clamped on the inlet connecting pipe (401) of the culture bag (4) when in use.
  10. 10. The device according to claim 1, further comprising a first support (7) and a second support, the first support (7) being adapted to house the culture medium bottle (3) and the peristaltic pump (2), the culture medium bottle (3) being, in use, inverted on the first support (7); The second support frame is used for supporting the fluid infusion tube (101) so as to ensure that the outlet of the fluid infusion tube (101) and the inlet of the culture bag (4) are kept opposite in position.

Description

Closed fluid infusion device for suspension cell culture Technical Field The utility model relates to the technical field of cell culture, in particular to a closed fluid infusion device for suspension cell culture. Background This section is intended to provide a background or context to the embodiments of the application that are recited in the claims. The description herein is not admitted to be prior art by inclusion in this section. Cell culture technology is a technology that mimics in vitro the in vivo environment, allowing cells to survive, grow, reproduce and maintain their structure and function. The method plays an important role in the fields of basic research, drug screening and toxicity test, biological product production, regenerative medicine, tissue engineering and the like, and has wide application prospect. In the cell culture process, fluid infusion operation is one of the key links. It is directly related to the efficiency and quality of cell culture. In the existing cell culture process, a commonly used fluid infusion method is to use a syringe, put the syringe in a syringe bracket to connect with a liquid inlet of a cell culture bag, and pour the culture medium into the syringe. Above-mentioned manual fluid infusion mode syringe and cultivate bag junction easily drops, uses the pipette or emptys easily to cause great error according to the blake bottle scale, needs to empty personnel more alternately many times, and the operation is complicated inapplicable to large-scale cultivation, spills easily when empting the culture medium and leaks, and many times open operation cells and culture medium pollution risk are high moreover. Disclosure of Invention The utility model aims to provide a closed fluid infusion device for suspension cell culture, which realizes closed, accurate and efficient fluid infusion operation in the cell culture process. The application discloses a closed fluid infusion device for suspension cell culture, which comprises a fluid transfer device and a peristaltic pump: The liquid transferring device comprises a liquid supplementing pipe, a first interface device and a second interface device, wherein one end of the liquid supplementing pipe is connected to a culture medium bottle through the first interface device formed by a medical thread conversion cover, a one-way valve and a filter, the other end of the liquid supplementing pipe is connected to a culture bag through the second interface device, and a closed system is formed among the culture medium bottle, the liquid transferring device and the culture bag; the peristaltic pump is used for pumping the liquid in the culture medium bottle into the culture bag unidirectionally at a uniform speed, and the liquid supplementing pipe is arranged in the peristaltic pump when the peristaltic pump is used. In a preferred embodiment, in the first interface device, the medical thread conversion cover is matched with the outlet of the culture medium bottle in size and sleeved at the outlet of the culture medium bottle, the cover cap of the medical thread conversion cover is provided with two round holes, one end of the fluid infusion tube and one end of the one-way valve are respectively connected outside the cover cap through the two round holes, and the other end of the one-way valve is connected with the filter; The one-way valve is used for controlling air to flow from the outside to the inside of the culture medium bottle, and the filter is used for filtering impurities in the air. In a preferred embodiment, the one-way valve is formed by a plurality of sections of pipe bodies with different diameters, and a circular one-way valve clack is arranged in one of the pipe bodies with different diameters; in a preferred embodiment, the one-way valve is formed by three sections of pipe bodies with different diameters, and a circular one-way valve clack is arranged in the middle one of the three sections of pipe bodies with different diameters; The filter is formed by sequentially connecting a connecting pipe, a disc-shaped structure and an inlet circular pipe, wherein a filtering membrane is arranged in the disc-shaped structure; The one-way valve is connected with the connecting pipe of the filter. In a preferred embodiment, the device further comprises a scale; the scale is used for weighing the mass of the culture bag in real time in the process of replenishing liquid and monitoring the mass change of the liquid in the culture bag. In a preferred embodiment, a controller is also included, the controller being electrically connected to the scale and the peristaltic pump for receiving a mass signal from the scale and sending a stop signal to stop the peristaltic pump when the mass of the culture bag reaches a desired mass. In a preferred embodiment, the peristaltic pump receives a mass signal from the scale and stops operating when the mass of the culture bag reaches a desired mass, and the peristaltic pump has a flow contr