CN-224212668-U - Human organ complete culture system

Abstract

The utility model discloses a human organ complete culture system which comprises a bottom plate, a pore plate layer, a bottoming reagent layer and a working liquid layer which are sequentially arranged from bottom to top, wherein the bottoming reagent layer and the working liquid layer are respectively provided with a plurality of reagent grooves, the reagent grooves of the bottoming reagent layer and the reagent grooves of the working liquid layer are mutually yin-yang grooves, the pore plate layer consists of a plurality of pore plates, a cover plate is arranged on the pore plate, a vent pipe is arranged on the upper end surface of the cover plate, the pore plate is provided with a vent hole, the vent pipe and the vent hole are respectively positioned at two opposite angles of the pore plate, the vent pipe of the lower pore plate is connected with the vent hole of the upper pore plate so that the flow direction of gas between the upper pore plate and the lower pore plate is Z-shaped, and the bottom plate, the uppermost pore plate, the bottoming reagent layer and the working liquid layer are connected through buckles. According to the utility model, through layered design, the pore plate layers are provided with the oxygen inlet and outlet channels, and the layers cooperate to jointly maintain the organoid culture environment.

Inventors

• TANG JUNNAN
• ZHANG JINYING
• HOU YACHEN
• GUO JIACHENG
• Mei Changkuan
• WU YANGYANG
• Wu Kanghui
• YANG YABING
• CHEN MINHAO

Assignees

• 郑州大学

Dates

Publication Date

20260508

Application Date

20250430

Claims (4)

1. 1. The human organ complete culture system comprises a bottom plate, a pore plate layer, a bottom-paving reagent layer and a working liquid layer which are sequentially arranged from bottom to top, and is characterized in that the bottom-paving reagent layer and the working liquid layer are respectively provided with a plurality of reagent grooves, the reagent grooves of the bottom-paving reagent layer and the reagent grooves of the working liquid layer are mutually yin-yang grooves, the pore plate layer consists of a plurality of pore plates, transparent plates are arranged around the pore plate, a cover plate is arranged on the upper end face of the cover plate, vent pipes for introducing gas required by cell growth into the pore plate are arranged on the upper end face of the cover plate, air outlets are arranged on the pore plate, the vent pipes and the air outlets are respectively positioned at two diagonal positions of the pore plate, the vent pipes of the pore plate at the lower layer are connected with the air outlets of the pore plate at the upper layer, so that the flow direction of the gas between the pore plate at the upper layer and the pore plate at the lower layer is Z-shaped, the pore plate at the uppermost layer and the bottom-paving reagent layer, and the bottom-paving reagent layer are connected through buckles.
2. 2. The human organoid complete culture system according to claim 1, wherein the air inlet of the air pipe on the uppermost orifice plate is an external air inlet, and the air outlet of the lowermost orifice plate is provided with an air outlet pipe for air outflow so as to exhaust air in the orifice plate layer.
3. 3. The human organoid complete culture system according to claim 1, wherein the reagent tank is provided on the left or right side of the upper end surface of the bottoming reagent layer, and correspondingly, the reagent tank is provided on the right or left side of the lower end surface of the working liquid layer.
4. 4. The complete human organoid culture system according to claim 1, wherein outer connecting struts are arranged at the outer sides of four corners of each layer of the well plate, and the upper layer of the well plate and the lower layer of the well plate are connected through the outer connecting struts.

Description

Human organ complete culture system Technical Field The utility model relates to a culture system, in particular to a human organ complete culture system. Background Human organ culture is used as a leading edge technology in the field of life science, and has irreplaceable significance for disease research, drug research and development, regenerative medicine and the like. The organoid is a three-dimensional tissue analogue formed by stem cells or progenitor cells in vitro, can highly simulate the structure and function of organs in vivo, and provides an ideal experimental model for deep exploration of physiological and pathological mechanisms of human bodies, development of personalized medicine screening and development of novel treatment means. In human organoids, the culture medium is one of the key factors. In recent years, human organoid complete medium technology has advanced. Some traditional culture mediums are improved, and various growth factors, cytokines and nutritional ingredients are added, so that the growth requirements of organoids are met to a certain extent. However, the culture effect of the conventional culture medium is unstable. There is often a difference in the results when different laboratories use the same formulation of medium to culture the same type of organoids. This is mainly due to the poor stability of some components of the medium, such as certain growth factors, which are prone to inactivation during storage and use. Meanwhile, the production process and quality control standard of the culture medium are not uniform enough, so that the quality of the culture medium in different batches is uneven. Taking intestinal organoids culture as an example, the intestinal organoids cultured by different batches of culture mediums have obvious differences in morphology, cell composition and functions, so that experimental results are difficult to repeat, and wide application of organoid technology in scientific research and clinical fields is prevented. Furthermore, the cost of the existing culture medium is high. Many components in the culture medium are expensive, such as some recombinant growth factors and special nutrients, which not only increase the cost of scientific research, but also limit the popularization of the culture medium in large-scale clinical application. In addition, complex components and stringent production conditions also increase the production costs of the culture medium, making human organoid culture techniques difficult to popularize. In an attempt to solve these problems, many difficulties are faced. On the one hand, the molecular mechanism of organoid growth is still not well understood, and it is difficult to precisely determine the optimal composition of the culture medium. On the other hand, development of new medium components or optimization of existing components requires a large amount of experimental verification and long-term study, and is costly and long-term. Meanwhile, establishing unified culture medium quality control standards faces various challenges such as technology and supervision. Disclosure of utility model The utility model aims to solve the key problems existing in the prior art of a human organ complete culture medium and provides a human organ complete culture system. In order to achieve the above purpose, the present utility model adopts the following technical scheme: The utility model provides a human organ complete culture system, includes bottom plate, aperture plate layer, shop bottom reagent layer and the working solution layer that from bottom to top set gradually, shop bottom reagent layer and working solution layer all are equipped with a plurality of reagent grooves, and shop bottom reagent layer's reagent groove and working solution layer's reagent groove each other are negative and positive groove, the aperture plate layer comprises a plurality of layers of aperture plate, is equipped with the transparent plate around the aperture plate, and the transparent plate upper cover is equipped with the apron, and the up end of apron is equipped with the breather pipe that is used for letting in cell growth required gas to the aperture plate, is equipped with the gas outlet on the aperture plate, thereby breather pipe and gas outlet are located two diagonal positions department of aperture plate respectively, thereby the breather pipe of lower floor's aperture plate and the gas outlet connection of upper strata aperture plate make the flow direction between upper and lower floor's aperture plate Z font, between bottom plate and the lower floor's aperture plate, between the uppermost layer aperture plate and the shop bottom reagent layer, between shop bottom reagent layer and the working solution layer all through the buckle connection. Further, the air inlet of the vent pipe on the uppermost orifice plate is an external gas inlet, and the air outlet of the lowermost orifice plate is provided with an air outlet pipe for