CN-224212670-U - Cell clone loop culture dish

Abstract

The utility model discloses a cell clone loop culture dish, which relates to the technical field of cell culture, and comprises a cell culture plate, wherein the cell culture plate with uniform specification is adopted; the method is characterized by comprising a bottom plate, wherein the bottom plate is arranged on the bottom surface of a cell culture plate, a cell cloning ring is arranged in the cell culture plate, a top cover is arranged at the upper end of the cell culture plate, the bottom plate and the cell cloning ring are fixed through magnetic attraction, the two have different colors, and the cell cloning ring is arranged in a culture tank of the cell culture plate. Ensuring that the cells obtained after digestion and elution are all from the same cell clone cluster, and achieving the purpose of accurately selecting the monoclonal cell cluster.

Inventors

• WANG YIYANG
• SHAO XIANYUN
• WEN JUNHAO

Assignees

• 暨南大学

Dates

Publication Date

20260508

Application Date

20250526

Claims (4)

1. 1. A cell cloning loop culture dish comprises a cell culture plate (100), wherein the cell culture plate is uniform in specification, and is characterized by comprising a bottom plate (200), wherein the bottom plate (200) is arranged on the bottom surface of the cell culture plate (100), a cell cloning loop (300) is arranged in the cell culture plate (100), a top cover (400) is arranged at the upper end of the cell culture plate (100), the bottom plate (200) and the cell cloning loop (300) are fixed through magnetic attraction, color difference exists between the bottom plate and the cell cloning loop, and the cell cloning loop (300) is arranged in a culture groove (101) of the cell culture plate (100).
2. 2. The cell clone loop culture dish according to claim 1, wherein the bottom plate (200) is provided with a plurality of embedded placing tables (201), and edges of the bottom plate (200) limit the cell culture plate (100) through buckles (202).
3. 3. The cell cloning ring set culture dish according to claim 1, wherein the cell cloning ring (300) is provided with a glass ring (301), a magnetic ring (302) and a silica gel ring (303) in sequence from top to bottom; the glass ring (301) is colorless and transparent; The magnetic ring (302) is made of magnetic materials; The silica gel ring (303) is made of soft materials.
4. 4. The cell clone loop culture dish according to claim 2, wherein the inside of the placing table (201) is provided with an LED lamp emitting 350nm ultraviolet light.

Description

Cell clone loop culture dish Technical Field The utility model relates to the technical field of cell culture, in particular to a cell clone loop culture dish. Background The existing cell cloning ring for selecting the monoclonal cell colony is composed of a common glass ring, and is placed on a preselected monoclonal cell colony growing on a cell culture plate when in use, so that the range of digestion and elution of cells when extracting the cells is limited. However, this tool is too simple in structure to be effectively immobilized on a cell culture plate and seal-add pancreatin to digest extracellular junctions. Resulting in the possibility of cloning ring shift, pancreatic enzyme extravasation, and the collection of cells from non-target clonal colonies during the procedure, resulting in unpredictable cell culture problems during subsequent experimental procedures. Disclosure of utility model Some simplifications or omissions may be made in this section as well as in the description of the utility model and in the title of the utility model, which may not be used to limit the scope of the utility model. The present utility model has been made in view of the problems described or existing in the prior art. Therefore, the utility model aims to provide a cell cloning loop culture dish which solves the technical problems of unstable positioning, easy leakage, unclear display of cell colonies and the like of the existing cell cloning loop. In order to solve the technical problems, the utility model provides the following technical scheme that the cell cloning loop culture dish comprises a cell culture plate and a cell culture plate with uniform specification; The cell culture plate comprises a bottom plate, wherein the bottom plate is arranged on the bottom surface of the cell culture plate, a cell cloning ring is arranged in the cell culture plate, a top cover is arranged at the upper end of the cell culture plate, the bottom plate and the cell cloning ring are fixed through magnetic attraction, the two are different in color, and the cell cloning ring is arranged in a culture tank of the cell culture plate. As a preferable scheme of the cell clone loop culture dish, the bottom plate is provided with a plurality of embedded placing tables, and the edge of the bottom plate limits the cell culture plate through a buckle. As a preferable scheme of the cell cloning ring sleeved culture dish, the cell cloning ring is provided with a glass ring, a magnetic ring and a silica gel ring from top to bottom in sequence; the glass ring is colorless and transparent; The magnetic ring is made of magnetic materials; The silica gel ring is soft. As a preferable scheme of the cell clone loop culture dish, the bottom plate and the upper end face of the placing table are both coated with colors. The utility model has the beneficial effects that the cell cloning ring set provided by the utility model can better fix the cell cloning ring at a target position, can better seal pancreatin of digested and eluted cells, and can clearly display selected cell colonies, and the device can better help scientific researchers to accurately and efficiently select and extract monoclonal cell colonies. Drawings In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein: FIG. 1 is an overall schematic of a cell clone loop culture dish; FIG. 2 is a schematic diagram of the resolution of a cell clone loop culture dish; FIG. 3 is a schematic diagram of a cell cloning loop culture dish; FIG. 4 is a schematic representation of the cell culture plate index of a cell clone loop culture dish. The marks in the figure: 100. Cell culture plate, 101, culture tank, 200, bottom plate, 201, placing table, 202, fastener, 300, cell cloning ring, 301, glass ring, 302, magnetic ring, 303, silica gel ring, 400, top cover. Detailed Description In order that the described objects, features and advantages of the utility model will be readily understood, a more particular description of the utility model will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present utility model, but the present utility model may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present utility model is not limited to the specific embodiments disclosed below. Further, reference herein to "one embodiment" or "an embodiment" means that a particul