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CN-224212671-U - NK cell culture device

CN224212671UCN 224212671 UCN224212671 UCN 224212671UCN-224212671-U

Abstract

The utility model discloses an NK cell culture device, which relates to the technical field of biological cell culture and comprises a base, a supporting structure, a power transmission system, a bearing and adjusting mechanism and a motion conversion assembly, wherein the base is used for bearing and stabilizing other parts, a first supporting seat is fixedly arranged on the base and is used for supporting a motor and a transmission part thereof, the motor drives a first gear to rotate through an output shaft, the first gear is meshed with a first rotating shaft, one end of the first rotating shaft is connected with a second gear, the second gear is connected with a rotating plate through a key slot, the rotating plate is connected with a sliding block through a second rotating shaft, the sliding block slides along a rack, and the rack is fixed on a fixed guide rail. The NK cell culture device provided by the utility model effectively solves the problem of cell sedimentation caused by the action of gravity, promotes uniform cell distribution, improves culture efficiency, reduces the operation frequency of experimenters, reduces pollution risks, and meets the requirement of diversified experiments.

Inventors

  • JIE ZHENWANG
  • Lin Gaokun
  • LIN YUTING

Assignees

  • 苏州厚朴惠康生物医药科技有限公司

Dates

Publication Date
20260508
Application Date
20250528

Claims (8)

  1. 1. The NK cell culture device is characterized by comprising a base (1), a first supporting seat (2), a frame (3), a motor (4), an output shaft (5), a first gear (6), a first rotating shaft (8), a second gear (9), a rotating plate (10), a sliding block (12), a rack (13), a fixed guide rail (14), a sliding frame (15), a screw rod (21), a sliding base (23), a supporting cylinder (28) and an adjusting sliding groove (27), wherein the base (1) is used for bearing and stabilizing other parts, the first supporting seat (2) is fixedly arranged on the base (1) and is used for supporting the motor (4) and a transmission part thereof, the motor (4) drives the first gear (6) to rotate through the output shaft (5), the first gear (6) is meshed with the first rotating shaft (8), one end of the first rotating shaft (8) is connected with the second gear (9), the second gear (9) is connected with the rotating plate (10) through a key groove, the rotating plate (10) is connected with the sliding block (12) through the second rotating shaft (11), the sliding block (12) is fixed on the rack (13) along the rack (13), the sliding frame (15) is arranged on the fixed guide rail (14) and bears a second supporting seat (16) and other components, the third gear (19) is meshed with the screw rod (21) to convert rotary motion into linear motion, the sliding base (23) is driven to move horizontally, the supporting cylinder (28) is used for supporting the NK cell culture bag, and the adjusting sliding chute (27) is matched with the adjusting sliding seat (29) to adjust the position of the supporting cylinder (28).
  2. 2. An NK cell culture device according to claim 1, wherein the base (1) is made of rigid material and is designed with a plurality of mounting holes, the geometry of the base (1) being optimised to ensure balance when carrying the components.
  3. 3. An NK cell culture device according to claim 2, characterized in that the bottom surface of the base (1) is provided with an anti-slip mat to further enhance the overall balance and anti-vibration properties.
  4. 4. The NK cell culture device according to claim 1, wherein the frame (3) is made of a high-strength alloy material, and a plurality of reinforcing ribs are arranged inside the frame to enhance the deformation resistance, and a plurality of reserved holes are designed on the frame (3) for mounting subsequent components.
  5. 5. The NK cell culture apparatus according to claim 1, wherein the bottom surface of the slider (12) is provided with a ball structure to reduce friction, the top of the slider (12) is connected to the rotating plate (10) through a pin, and both ends of the pin are provided with anti-drop caps to prevent loosening or falling during operation.
  6. 6. The NK cell culture apparatus according to claim 1, wherein both sides of the slide frame (15) are provided with a limit protrusion, which cooperates with a groove of the fixed rail (14) to limit the movement range of the slide frame (15), and the bottom surface of the slide frame (15) is provided with a lubrication coating to reduce the friction coefficient.
  7. 7. The NK cell culture apparatus as defined in claim 1, wherein the surface of the screw (21) is hardened to improve wear resistance, the sliding base (23) restricts the movement path by means of a guide runner (24), and the width of the guide runner (24) is closely fitted to the convex portion of the sliding base (23).
  8. 8. The NK cell culture device as defined in claim 1, wherein the inner wall of the holding cylinder (28) is provided with an elastic gasket to protect the culture bag from damage.

Description

NK cell culture device Technical Field The utility model relates to the technical field of biological cell culture, in particular to an NK cell culture device. Background In the fields of biomedical research and clinical application, NK cell (natural killer cell) culture technology has important significance. NK cells are important immune cells and play a key role in anti-tumor, antiviral, immunoregulation and the like. However, the existing NK cell culture techniques still suffer from a number of drawbacks, particularly in terms of the design and use of the cell culture device. The conventional method generally comprises the steps of flatly placing a cell culture bag in an incubator, and easily settling NK cells to the bottom of the culture bag after aggregation due to the action of gravity, so that the cells are unevenly distributed, and the growth efficiency and activity of the cells are affected. In addition, in order to solve the problem of cell sedimentation, the experimenter needs to frequently open the incubator and perform a patting operation on the culture bag to re-disperse the cells. The repeated manual intervention not only increases the complexity of operation, but also brings potential pollution risks, and seriously influences the stability and safety of the culture process. Therefore, developing an NK cell culture apparatus that can effectively prevent cell sedimentation, reduce manual intervention, and improve culture efficiency is a technical problem currently in need of solving. The present utility model is based on the above background and aims to provide an innovative solution to overcome the deficiencies in the prior art. Disclosure of utility model Aiming at the technical problems that cells settle at the bottom of a culture bag due to the action of gravity in the existing NK cell culture process, the growth of the cells is affected, and pollution risks are brought by frequent operation of experimenters, the utility model provides an NK cell culture device for realizing dynamic regulation through a mechanical transmission mechanism. The device aims to solve the technical problems and improve the cell culture efficiency and the safety. The utility model provides an NK cell culture device which comprises a base, a supporting structure, a power transmission system, a bearing and adjusting mechanism and a motion conversion assembly. Wherein the base serves as a base support portion of the overall device for carrying and stabilizing other components. The base is made of rigid materials and is provided with a plurality of mounting holes so as to adapt to the fixing requirements in different experimental environments. Further, the geometry of the base is optimized to ensure that it remains balanced when carrying the components, thereby improving the reliability of the overall device. Further, the first supporting seat is fixedly arranged on the base and used for supporting the motor and related transmission parts thereof, and the frame is connected with the base and the first supporting seat and forms an integral frame structure. The frame is made of high-strength alloy materials, and a plurality of reinforcing ribs are arranged in the frame to strengthen the deformation resistance. In particular, the frame is also designed with a plurality of reserved hole sites for installing subsequent components, thereby facilitating assembly and maintenance. Further, the motor drives the first gear to rotate through the output shaft, the first gear is meshed with the first rotating shaft, and one end of the first rotating shaft is connected with the second gear. The first gear and the second gear are standard involute gears so as to ensure stability and accuracy in the transmission process. The first rotating shaft is fixed in the first supporting seat through a bearing, and the bearing adopts a double-row deep groove ball bearing so as to bear radial and axial loads. Further, the second gear is connected with the rotating plate through a key slot so as to realize synchronous rotation movement. In particular, the rotating plate is connected with the sliding block through the second rotating shaft, and the sliding block slides along the rack. The rack is fixed on a fixed guide rail, and the fixed guide rail is arranged on the side face of the rack through bolts. The bottom surface of slider is provided with ball structure to reduce frictional force, improve sliding efficiency. Further, the top of the sliding block is connected with the rotating plate through a pin shaft, and anti-falling caps are arranged at two ends of the pin shaft so as to prevent loosening or falling in the running process. Further, the fixed guide rail guides the sliding block to move along a preset track, and the sliding frame is installed on the fixed guide rail and bears the second supporting seat and other components. Limiting protrusions are arranged on two sides of the sliding frame and matched with grooves of the fixed guide