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CN-224216555-U - Immunofluorescence staining tool kit

CN224216555UCN 224216555 UCN224216555 UCN 224216555UCN-224216555-U

Abstract

The utility model belongs to the technical field of immunofluorescence staining, and particularly relates to an immunofluorescence staining tool kit, which comprises a base, a cleaning module, a staining module and a cover plate, wherein the cleaning module is detachably arranged on the base and used for cleaning a used cover glass, the staining module is detachably arranged on the cleaning module and used for staining the cover glass, and the cover plate is detachably arranged on the staining module. The utility model is convenient to use and can ensure the dyeing effect. The evaporation of the reagent in the long-time incubation process can be reduced, so that the dyeing effect is more stable. And can guarantee independent staining when a plurality of cover slips are simultaneously incubated and transferred, and can avoid confusion of each other. The cleaning module can simultaneously meet different requirements of quick cleaning and long-time cleaning of the cover glass, can finish the addition of the reagent once, and omits the complicated step of replacing the reagent for many times. Meanwhile, the detachable design can enable the cleaning and drying of all parts to be easier, and the cover glass can be conveniently taken and placed and the cleaning liquid can be conveniently replaced.

Inventors

  • MAI XIAOTING
  • CHEN HAOYU
  • ZHANG PEIXIN
  • HUANG SHAOFEN
  • Chen Chongbo

Assignees

  • 汕头大学·香港中文大学联合汕头国际眼科中心

Dates

Publication Date
20260508
Application Date
20241129

Claims (10)

  1. 1. An immunofluorescent staining kit, comprising A base (1); The cleaning module is detachably arranged on the base (1) and is used for cleaning the used cover glass; The staining module is detachably arranged on the cleaning module and is used for staining the cover glass; And the cover plate (6) is detachably arranged on the dyeing module.
  2. 2. The immunofluorescence staining kit according to claim 1, wherein the washing module comprises a supporting plate (2), the supporting plate (2) is detachably mounted on the base (1), a washing seat (3) is fixedly connected to the supporting plate (2), two accommodating grooves (301) are formed in the washing seat (3), the two accommodating grooves (301) are arranged at intervals in parallel, a first washing groove (7) is detachably mounted in one accommodating groove (301), and a second washing groove (8) is detachably mounted in the other accommodating groove (301).
  3. 3. The immunofluorescent staining tool kit according to claim 2, wherein a plurality of first sub-grooves (701) are formed in the first cleaning groove (7), and the plurality of first sub-grooves (701) are arranged in an array along the length direction and the width direction of the first cleaning groove (7); A plurality of second sub-grooves (801) are formed in the second cleaning groove (8), and the plurality of second sub-grooves (801) are arranged in an array along the length direction and the width direction of the second cleaning groove (8).
  4. 4. The immunofluorescent staining tool kit according to claim 2, wherein the staining module comprises a first staining plate (4) and a second staining plate (5), wherein the first staining plate (4) is detachably mounted on the top end of the cleaning seat (3), the second staining plate (5) is detachably mounted on the top end of the first staining plate (4), and the cover plate (6) is detachably mounted on the top end of the second staining plate (5).
  5. 5. The immunofluorescence staining kit according to claim 4, wherein a boss I (401) is arranged at the top end of the first staining plate (4), a plurality of staining grooves (402) are formed in the boss I (401), and a plurality of staining grooves (402) are arranged in an array; The top end of the dyeing plate II (5) is provided with a boss II (501), a plurality of molding columns (502) are arranged on the boss II (501), and a plurality of molding columns (502) are arranged in an array.
  6. 6. The immunofluorescent staining tool kit according to claim 5, wherein the top end of the base (1) is provided with a storage cavity (101), and the top end of the side wall of the storage cavity (101) is provided with a circular protrusion (102).
  7. 7. The immunofluorescent staining tool kit according to claim 6, wherein the bottom end of the supporting plate (2) is provided with a groove I (201), and the groove I (201) is matched with the annular protrusion I (102).
  8. 8. The immunofluorescent staining tool kit according to claim 7, wherein the first staining plate (4) is provided with a second groove (403), the outer sides of the two accommodation grooves (301) are provided with a second annular protrusion (302), and the second groove (403) is matched with the second annular protrusion (302).
  9. 9. The immunofluorescent staining tool kit of claim 8, wherein the bottom end of the staining plate II (5) is provided with a groove III (503), and the groove III (503) is matched with the boss I (401).
  10. 10. The immunofluorescent staining tool kit according to claim 9, wherein a fourth groove (601) is formed in the bottom end of the cover plate (6), and the fourth groove (601) is matched with the second boss (501).

Description

Immunofluorescence staining tool kit Technical Field The utility model belongs to the technical field of immunofluorescence staining, and particularly relates to an immunofluorescence staining tool kit. Background Immunofluorescent staining techniques are based on the localization of fluorescently labeled antigens (antibodies) in response to antigen-antibody reactions with corresponding molecules in tissues or cells. The cell immunofluorescence staining sample which is commonly used at present is a cell slide, the used slide is generally a circular cover glass with the radius of 6mm, and the steps of cleaning, sealing, antibody incubation and the like are needed in the staining process. To save antibodies and reagents, the most common method is to reverse-snap the coverslip onto a pre-placed drop of liquid on the sealing film for washing, blocking and antibody incubation. However, because the sealing film is smooth, the displacement of the cover glass easily occurs when the sealing film is moved, so that a plurality of cover glass are overlapped or slide out of the sealing film, and meanwhile, insufficient cleaning is easily caused by too little liquid when the cover glass is cleaned. Therefore, there is a need to design an immunofluorescent staining kit to address the above-described issues. Disclosure of utility model It is an object of the present utility model to provide an immunofluorescent staining kit to solve the above problems. To achieve the above object, the present utility model provides an immunofluorescent staining kit comprising A base; The cleaning module is detachably arranged on the base and is used for cleaning the used cover glass; The staining module is detachably arranged on the cleaning module and is used for staining the cover glass; And the cover plate is detachably arranged on the dyeing module. The immunofluorescence staining tool kit based on the utility model comprises a supporting plate, wherein the supporting plate is detachably arranged on a base, a cleaning seat is fixedly connected to the supporting plate, two accommodating grooves are formed in the cleaning seat, the two accommodating grooves are arranged in parallel at intervals, a first cleaning groove is detachably arranged in one accommodating groove, and a second cleaning groove is detachably arranged in the other accommodating groove. Based on the immunofluorescence staining tool kit, a plurality of first sub-grooves are formed in the first cleaning groove, and the plurality of first sub-grooves are arranged in an array along the length direction and the width direction of the first cleaning groove; A plurality of second sub-tanks are arranged in the second cleaning tank, and the second sub-tanks are arranged in an array along the length direction and the width direction of the second cleaning tank. Based on the immunofluorescence staining tool kit disclosed by the utility model, the staining module comprises a staining plate I and a staining plate II, wherein the staining plate I is detachably arranged at the top end of the cleaning seat, the staining plate II is detachably arranged at the top end of the staining plate I, and the cover plate is detachably arranged at the top end of the staining plate II. Based on the immunofluorescence staining tool kit, the top end of the staining board I is provided with the boss I, a plurality of staining grooves are formed in the boss I, and a plurality of staining grooves are arranged in an array; The dyeing board is characterized in that a boss II is arranged at the top end of the dyeing board II, a plurality of shaping columns are arranged on the boss II, and a plurality of shaping columns are arranged in an array. Based on the immunofluorescence staining tool kit disclosed by the utility model, a storage cavity is formed in the top end of the base, and a circular protrusion is arranged on the top end of the side wall of the storage cavity. Based on the immunofluorescence staining tool kit disclosed by the utility model, the bottom end of the supporting plate is provided with the groove I, and the groove I is matched with the annular protrusion I. Based on the immunofluorescence staining tool kit disclosed by the utility model, the bottom end of the staining plate I is provided with the groove II, the outer sides of the two containing grooves are provided with the annular protrusions II, and the groove II is matched with the annular protrusions II. Based on the immunofluorescence staining tool kit disclosed by the utility model, the bottom end of the staining plate II is provided with the groove III, and the groove III is matched with the boss I. Based on the immunofluorescence staining tool kit disclosed by the utility model, the bottom end of the cover plate is provided with the groove IV, and the groove IV is matched with the boss II. Compared with the prior art, the utility model has the following advantages and technical effects: 1. The utility model is convenient to use