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CN-224216556-U - Fluorescent signal reading assembly

CN224216556UCN 224216556 UCN224216556 UCN 224216556UCN-224216556-U

Abstract

The utility model provides a fluorescent signal reading assembly, and relates to the technical field of optical signal reading. The fluorescent signal reading assembly comprises an optical filter and a reading box, wherein a storage through hole, an excitation through hole and a shooting through hole are formed in the side wall of the reading box at intervals, the center lines of the storage through hole, the excitation through hole and the shooting through hole are perpendicular to each other and are intersected at the same point, the optical filter is detachably arranged in the reading box corresponding to the shooting through hole, the centrifuge tube is placed in the reading box through the storage through hole, and when an excitation light source irradiates the centrifuge tube through the excitation through hole, a shooting device can shoot and read fluorescent signals of a sample in the centrifuge tube through the shooting through hole. The fluorescent signal reading assembly is simple in structure, low in manufacturing cost and simple to operate, and has no professional requirements on operators.

Inventors

  • CHEN WENWEN
  • LU XI

Assignees

  • 深圳大学

Dates

Publication Date
20260508
Application Date
20250421

Claims (10)

  1. 1. A fluorescent signal reading assembly for reading fluorescent signals from a sample in a centrifuge tube (10), comprising: A filter (100); A reading box (200), wherein the side wall of the reading box (200) is provided with a storage through hole (210), an excitation through hole (220) and a shooting through hole (230) at intervals, the center lines of the object placing through hole (210), the excitation through hole (220) and the shooting through hole (230) are mutually perpendicular and are intersected at the same point, and the optical filter (100) is detachably arranged in the reading box (200) corresponding to the shooting through hole (230); The centrifuge tube (10) is placed in the reading box (200) through the object placing through hole (210), and when a laser source irradiates the centrifuge tube (10) through the excitation through hole (220), a shooting device can shoot and read fluorescent signals of samples in the centrifuge tube (10) through the shooting through hole (230).
  2. 2. The fluorescent signal reading assembly according to claim 1, wherein the photographing through hole (230) is provided as an elongated hole extending in the Z direction, the reading box (200) is adjustably provided with a photographing auxiliary member (300), and the photographing auxiliary member (300) is provided with a photographing auxiliary hole (310) corresponding to the photographing through hole (230); The excitation through hole (220) is arranged to be a strip hole extending along the Z direction, an excitation auxiliary piece (400) is adjustably arranged on the reading box (200), and an excitation auxiliary hole (410) corresponding to the excitation through hole (220) is arranged on the excitation auxiliary piece (400).
  3. 3. The fluorescent signal reading assembly according to claim 2, wherein the photographing auxiliary member (300) is provided as a long strip plate corresponding to the photographing through hole (230) and detachably abuts against an inner wall of the photographing through hole (230); The shooting auxiliary piece (300) is provided with one shooting auxiliary hole (310), or a plurality of shielding shooting auxiliary holes (310) are arranged on the shooting auxiliary piece (300) at intervals along the Z direction.
  4. 4. The fluorescent signal reading assembly according to claim 2, wherein the reading box (200) is provided with a photographing auxiliary chamber (231) extending along the Z direction corresponding to the photographing through hole (230), the photographing auxiliary member (300) is provided as a flexible plate and is movably mounted in the photographing auxiliary chamber (231), and one photographing auxiliary hole (310) is provided on the photographing auxiliary member (300).
  5. 5. The fluorescent signal reading assembly according to claim 2, wherein the excitation aid (400) is arranged as a strip plate corresponding to the excitation through hole (220) and detachably abuts against an inner wall of the excitation through hole (220); The excitation auxiliary piece (400) is provided with one excitation auxiliary hole (410), or a plurality of shielding excitation auxiliary holes (410) are arranged on the excitation auxiliary piece (400) at intervals along the Z direction.
  6. 6. The fluorescent signal reading assembly according to claim 2, wherein the reading box (200) is provided with an excitation auxiliary chamber (221) extending along the Z direction corresponding to the excitation through hole (220), the excitation auxiliary member (400) is provided as a flexible plate and movably mounted in the excitation auxiliary chamber (221), and one excitation auxiliary hole (410) is provided on the excitation auxiliary member (400).
  7. 7. The fluorescent signal reading assembly according to claim 2, wherein the cross-sectional area of the excitation auxiliary hole (410) increases stepwise from the excitation through hole (220) to the direction of the center of the reading cartridge (200).
  8. 8. The fluorescent signal reading assembly according to any one of claims 1-7, further comprising a cover plate (500), the cover plate (500) being arranged to the outer wall of the reading cassette (200) in correspondence of the storage through hole (210).
  9. 9. The fluorescent signal reading assembly according to claim 8, further comprising a guiding and limiting assembly (600), wherein the cover plate (500) is movably arranged on the outer wall of the reading box (200) through the guiding and limiting assembly (600).
  10. 10. The fluorescent signal reading assembly according to claim 8, wherein a storage groove (240) is further formed on the outer wall of the reading box (200), and the storage groove (240) is concentrically arranged with the storage through hole (210) and forms a limit step in cooperation with the storage through hole (210); And/or an elastic limit ring is fixedly arranged on the inner side of the object placing through hole (210).

Description

Fluorescent signal reading assembly Technical Field The utility model relates to the technical field of optical signal reading, in particular to a fluorescent signal reading assembly. Background The light emitted by a substance after absorption of light or other electromagnetic radiation is known as fluorescence, also known as "fluorescence", and is a phenomenon of cold luminescence. In detection techniques such as in the medical detection field, fluorescent groups are often used as reporter molecules, for example nucleic acid fluorescent probes in the field of molecular diagnostics, which fluoresce when a target gene is amplified. The conventional fluorescent signal reading is usually realized by a fluorescent microscope, but when the fluorescent microscope reads the fluorescent signal, an operator is required to perform professional operation, a good fluorescent signal image can be obtained by adjusting various complex parameters in the fluorescent microscope, the professional requirement on the operator is high, and the fluorescent microscope is high in manufacturing cost, so that the fluorescent signal can not be popularized in a region with a lag in economy. Disclosure of utility model The utility model aims to provide a fluorescent signal reading assembly which has the advantages of simple structure, low cost and simple operation and has no professional requirements on operators. To achieve the purpose, the utility model adopts the following technical scheme: a fluorescent signal reading assembly for reading fluorescent signals of a sample in a centrifuge tube, comprising: a light filter; The side wall of the reading box is provided with a storage through hole, an excitation through hole and a shooting through hole at intervals, the center lines of the storage through hole, the excitation through hole and the shooting through hole are mutually perpendicular, the optical filters are detachably arranged in the reading box corresponding to the shooting through holes; The centrifuge tube is placed in the reading box through the object placing through hole, and when the excitation light source irradiates to the centrifuge tube through the excitation through hole, the shooting device can shoot and read fluorescent signals of samples in the centrifuge tube through the shooting through hole. As a further technical scheme, the shooting through hole is a long strip hole extending along the Z direction, a shooting auxiliary piece is adjustably arranged on the reading box, and a shooting auxiliary hole is arranged on the shooting auxiliary piece corresponding to the shooting through hole; The excitation through hole is arranged to be a strip hole extending along the Z direction, an excitation auxiliary piece is adjustably arranged on the reading box, and an excitation auxiliary hole is arranged on the excitation auxiliary piece corresponding to the excitation through hole. As a further technical scheme, the shooting auxiliary piece is arranged as a strip plate corresponding to the shooting through hole and is detachably abutted against the inner wall of the shooting through hole; The auxiliary shooting device is characterized in that one auxiliary shooting hole is formed in the auxiliary shooting piece, or a plurality of auxiliary shooting holes capable of being blocked are formed in the auxiliary shooting piece at intervals along the Z direction. As a further technical scheme, the reading box is provided with a shooting auxiliary cavity extending along the Z direction corresponding to the shooting through hole, the shooting auxiliary piece is arranged as a flexible plate and movably mounted in the shooting auxiliary cavity, and the shooting auxiliary piece is provided with a shooting auxiliary hole. As a further technical scheme, the excitation auxiliary piece is arranged as a strip plate corresponding to the excitation through hole and is detachably abutted against the inner wall of the excitation through hole; The excitation auxiliary piece is provided with one excitation auxiliary hole, or a plurality of shielding excitation auxiliary holes are arranged on the excitation auxiliary piece at intervals along the Z direction. As a further technical scheme, the reading box is provided with an excitation auxiliary cavity extending along the Z direction corresponding to the excitation through hole, the excitation auxiliary piece is arranged as a flexible plate and movably mounted in the excitation auxiliary cavity, and the excitation auxiliary piece is provided with one excitation auxiliary hole. As a further technical solution, the cross-sectional area of the excitation auxiliary hole increases gradually from the excitation through hole to the direction of the center of the reading cassette. As a further technical scheme, the fluorescent signal reading assembly further comprises a cover plate, and the cover plate is arranged on the outer wall of the reading box corresponding to the object placing through hole. As a