CN-224216623-U - Electrochemiluminescence-electrophoresis incubation system

CN224216623UCN 224216623 UCN224216623 UCN 224216623UCN-224216623-U

Abstract

The utility model relates to the technical field of biosensing, and discloses an electrochemiluminescence-electrophoresis incubation system which comprises an electrochemiluminescence-electrophoresis analysis module, a first container, a first cover body, a second container, a second cover body, a working electrode, a platinum plate, a conduit, a valve, a reference electrode and a counter electrode, wherein the first container is connected with the second container through the conduit, the conduit is provided with the valve, the platinum plate is arranged at the bottom of the first container, the first cover body is detachably arranged at the top of the first container, the working electrode is arranged on the first cover body, the reference electrode and the counter electrode are both arranged on the second cover body, the second cover body is detachably arranged at the top of the second container, and the platinum plate, the working electrode, the reference electrode and the counter electrode are all connected with the electrochemiluminescence-electrophoresis analysis module. The utility model can remarkably simplify the experimental flow, quicken the detection speed, greatly shorten the detection time, and simultaneously avoid the error of experimental detection results caused by the interference of factors such as environment and the like on the working electrode in the transfer process.

Inventors

WU ZHANGHONG
ZANG GUANGCHAO
CHEN HONGYU
LI XINHANG
ZUO XIANGYU
Han Keer
Kang Shouheng
HUANG XIAORUI
WU JIASHENG
TANG SHENGNAN

Assignees

重庆医科大学

Dates

Publication Date: 20260508
Application Date: 20250526

Claims (10)

1. The electrochemiluminescence-electrophoresis incubation system is characterized by comprising an electrochemiluminescence-electrophoresis analysis module, a first container, a first cover body, a second container, a second cover body, a working electrode, a platinum disk, a conduit, a valve, a reference electrode and a counter electrode, wherein the first container is connected with the second container through the conduit, the valve is arranged on the conduit, the platinum disk is arranged at the bottom of the first container, the first cover body is detachably arranged at the top of the first container, the working electrode is arranged on the first cover body, when the first cover body is arranged at the top of the first container, the part of the working electrode stretches into the first container, the reference electrode and the counter electrode are both arranged on the second cover body, the second cover body is detachably arranged at the top of the second container, when the second cover body is arranged at the top of the second container, the reference electrode and the part of the counter electrode stretch into the first container, and the working electrode are both connected with the electrochemiluminescence analysis module.
2. The electrochemiluminescence-electrophoresis incubation system according to claim 1, further comprising a chemiluminescent detection camera bellows, wherein the first container and the second container are both disposed in the chemiluminescent detection camera bellows, the chemiluminescent detection camera bellows is provided with a wiring hole, the electrochemiluminescence-electrophoresis analysis module is disposed on the outer side of the chemiluminescent detection camera bellows, and the platinum plate, the working electrode, the reference electrode and the counter electrode are all connected with the electrochemiluminescence-electrophoresis analysis module by passing through the wiring hole in a sealing manner through a wire.
3. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein the first cover is provided with a liquid well.
4. The electrochemiluminescence-electrophoresis incubation system according to claim 2, further comprising a support assembly disposed in the chemiluminescent detection camera bellows, the support assembly comprising a support column and a cross-bar fixedly connected to the support column and perpendicular to the support column, the cross-bar having a first through-hole and a second through-hole disposed therein, the first container and the second container being disposed in the first through-hole and the second through-hole, respectively.
5. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein the electrochemiluminescence-electrophoresis analysis module comprises a control module and a power module, the platinum plate and the counter electrode are connected with the negative electrode of the power module through one wire, the working electrode and the reference electrode are connected with the positive electrode of the power module through one wire, an ammeter is arranged on a loop of connecting the working electrode with the positive electrode of the power module, a voltmeter is arranged on a loop of connecting the reference electrode with the positive electrode of the power module, and a first switch, a second switch, a third switch and a fourth switch are respectively arranged on wires of connecting the platinum plate, the working electrode, the reference electrode and the counter electrode with the power module, signal output ends of the ammeter and the voltmeter are connected with signal input ends of the control module, and signal output ends of the control module are connected with signal input ends of the power module, the first switch, the second switch, the third switch and the fourth switch.
6. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein the working electrode is a magnetic glassy carbon electrode.
7. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein the reference electrode is an Ag/AgCl electrode.
8. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein the counter electrode is a platinum wire electrode.
9. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein lead ends of the platinum plate, the working electrode, the reference electrode and the counter electrode are respectively connected with one end of a wire through an electrode clamp, and the other end of the wire is connected with the electrochemiluminescence-electrophoresis analysis module.
10. The electrochemiluminescence-electrophoresis incubation system according to claim 1, wherein the conduit is a glass conduit and the valve is a glass valve.

Description

Electrochemiluminescence-electrophoresis incubation system Technical Field The utility model relates to the technical field of biological sensing, in particular to an electrochemiluminescence-electrophoresis incubation system. Background In the prior art, china patent with publication number CN117129549A discloses a system, a sensor and a method for detecting CEA based on electrophoresis and ECL principles, which relate to swimming devices. The electrophoresis device is an electrolysis cup with a platinum wire disc at the bottom, the platinum wire disc is connected with the negative electrode of a power supply of the electrophoresis apparatus, the working electrode of the electrochemiluminescence immunosensor is connected with the positive electrode of the power supply, and a substance to be detected is driven to the surface of the electrode to be enriched by upward electric field force. However, the electrophoresis device and ECL detection device in this technique are two separate devices. Thus, the prior art requires the working electrode to be exchanged back and forth between two different devices during the electrophoresis incubation and detection process. Not only can the experimental process be more complicated, but also the electrode can be polluted in the transfer process, so that the possibility of nonspecific binding of the electrode surface is increased, and the experimental complexity is greatly increased. Disclosure of utility model In view of the above-mentioned drawbacks of the prior art, the present utility model aims to provide an electrochemiluminescence-electrophoresis incubation system, which integrates an electrophoresis device and an ECL detection device for the first time, wherein one part is an electrophoresis area, the other part is a reference and platinum wire electrode, and the electrophoresis and detection are separated by a controllable glass valve, so that the integration of electrophoresis and detection is realized, and the experimental process is greatly simplified. In addition, according to the principle of luminescence by voltage generated by electrochemiluminescence, the utility model creatively combines the electrophoresis apparatus with the electrochemiluminescence apparatus, and provides new thinking and direction for commercial ECL detection. The utility model adopts the following technical scheme: The utility model provides an electrochemiluminescence-electrophoresis incubation system, includes electrochemiluminescence-electrophoresis analysis module, first container, first lid, second container, second lid, working electrode, platinum dish, pipe, valve, reference electrode and counter electrode, first container passes through the pipe connection the second container, set up on the pipe the valve, the platinum dish set up in the bottom of first container, first lid detachably set up in the top of first container, the working electrode install in on the first lid, when first lid set up in the top of first container, part of working electrode stretches into in the first container, reference electrode and counter electrode all install in on the second lid, second lid detachably set up in the top of second container, when the second lid set up in the top of second container, reference electrode and counter electrode all stretch into in the second container, the reference electrode all stretches into in the second container, the working electrode all with electrophoresis module. Preferably, in the above electrochemiluminescence-electrophoresis incubation system, the system further comprises a chemiluminescence detection camera bellows, the first container and the second container are both disposed in the chemiluminescence detection camera bellows, the chemiluminescence detection camera bellows is provided with a wiring hole, the electrochemiluminescence-electrophoresis analysis module is disposed at the outer side of the chemiluminescence detection camera bellows, and the platinum plate, the working electrode, the reference electrode and the counter electrode are all connected with the electrochemiluminescence-electrophoresis analysis module by penetrating through the wiring hole in a sealing manner through a wire. Preferably, in the electrochemiluminescence-electrophoresis incubation system, the first cover body is provided with a liquid hole. Preferably, in the electrochemiluminescence-electrophoresis incubation system, the system further comprises a support assembly, the support assembly is arranged in the chemiluminescence detection camera bellows, the support assembly comprises a support column and a cross rod, the cross rod is fixedly connected with the support column and is perpendicular to the support column, a first through hole and a second through hole are formed in the cross rod, and the first container and the second container are respectively arranged in the first through hole and the second through hole. Preferably, in the electrochemiluminescence-electroph