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CN-224216706-U - Immunodetection reagent card

CN224216706UCN 224216706 UCN224216706 UCN 224216706UCN-224216706-U

Abstract

The utility model discloses an immunodetection reagent card, which comprises a shell and a liquid channel positioned in the shell, wherein the shell is formed by enclosing a bottom plate and a cover plate, a sample adding hole and an observation window are arranged on the cover plate, the immunodetection reagent card also comprises a filter pad used for guiding and filtering sample solution from the sample adding hole to the liquid channel, the liquid channel is at least 1 micro-channel for the sample solution to pass through, a cross micro-array which is arranged by adopting a capillary force principle and used for driving the sample solution to flow in the micro-channel is arranged in the micro-channel, a first marking area, a second marking area and a third marking area are sequentially arranged in the micro-channel along the flowing direction of the sample solution, a marking antibody, a capturing antibody 1 and a capturing antibody 2 are sequentially coated on the areas respectively, and the observation window can observe at least the second marking area and the third marking area. The method has the advantages of 1) simplifying the whole structure of the chip and reducing the production complexity and cost. 2, the process controllability and the detection sensitivity are improved.

Inventors

  • Ji Huijiao
  • ZHANG QINGWEN

Assignees

  • 西南医科大学

Dates

Publication Date
20260508
Application Date
20250423

Claims (8)

  1. 1. The immunodetection reagent card comprises a shell and a liquid channel positioned in the shell, wherein the shell is formed by encircling a bottom plate (1) and a cover plate (2), a sample adding hole (201) and an observation window (202) are arranged on the cover plate (2), the immunodetection reagent card further comprises a filter pad (3) used for guiding and filtering sample solution to the liquid channel through the sample adding hole (201), and is characterized in that the liquid channel is at least 1 micro-channel (4) for allowing the sample solution to pass through, a cross micro-array (404) which is arranged according to the capillary force principle and is used for driving the sample solution to flow in the micro-channel (4) is arranged in the micro-channel (4), a first marking area (401), a second marking area (402) and a third marking area (403) are sequentially arranged in the micro-channel (4) along the flowing direction of the sample solution, a marked antibody, a capturing antibody 1 and a capturing antibody 2 are sequentially coated on the areas respectively, and the observation window (202) can observe at least the second marking area (402) and the third marking area (403).
  2. 2. The immunoassay reagent card of claim 1, wherein the tip of the microchannel (4) is provided with a liquid absorbing pad (5) for absorbing the sample solution passing through the microchannel (4).
  3. 3. The immunodetection reagent card of claim 2, wherein a first air hole (203) connected with the filter pad (3) and a second air hole (204) connected with the liquid absorbing pad (5) are arranged on the cover plate (2) in a penetrating way.
  4. 4. The immunodetection reagent card of claim 1, wherein the micro-channel (4) is formed by arranging a micro-channel groove on the bottom plate (1) in advance, and sealing the cover plate (2) on the top of the micro-channel groove by the bottom plate (1) and the cover plate (2) in an ultrasonic bonding mode, so that the micro-channel groove and the cover plate (2) are enclosed to form the micro-channel (4).
  5. 5. The immunodetection reagent card of claim 1, wherein the width of said microchannel (4) is 20-40 mm and the height is 30-50 μm.
  6. 6. The immunoassay reagent card of claim 5, wherein the cross microarray (404) is a cylindrical array in which the micro-cylinders (4041) are axially perpendicular to the flow direction of the sample solution in the micro-channel (4), the diameter of each micro-cylinder (4041) of the cylindrical array is 60-100 μm, the distance between adjacent micro-cylinders (4041) is 100-200 μm, and the height of each micro-cylinder (4041) is smaller than the height of the micro-channel (4).
  7. 7. The immunodetection reagent card of claim 1-6, wherein said viewing window (202) is transparent.
  8. 8. The immunodetection reagent card of claim 1-6, further comprising a light-shielding label (6) covering the cover plate (2).

Description

Immunodetection reagent card Technical Field The utility model relates to an in-vitro immunodetection technology, in particular to an immunodetection reagent card. Background The immunodetection technology relies on the specific binding principle of antigen and antibody, and combines various reagent card structures, such as immunochromatography and immunomicrofluidics, to realize the aim of detection. However, the structural design of the reagent card in the traditional immunochromatography technology is complex, at least 4 raw materials are required to be stacked in multiple layers, and the immunological binding efficiency is easily affected by the layout and the manufacturing process of each part of the reagent card body. In contrast, although the immunomicrofluidic technology simplifies the structural stacking of the reagent card, a plurality of micro-functional structural areas are required to be designed, and higher requirements are put on the injection molding production process, so that the production yield is low, and the wide clinical application of the immunomicrofluidic technology is hindered. In addition, the high production costs limit its market acceptance. Therefore, the traditional chromatography technology and the microfluidic technology are integrated, and a more efficient chip is developed, so that the method has a certain application prospect. Disclosure of utility model The utility model provides an immunodetection reagent card for solving the problems of strong dependence on a multilayer film structure, complex process, high production difficulty, high cost, difficult popularization, poor antigen/antibody coating process stability and difficult permeability control of the traditional chromatography technology. The technical scheme includes that the immunodetection reagent card comprises a shell and a liquid channel located in the shell, wherein the shell is formed by enclosing a bottom plate and a cover plate, a sample adding hole and an observation window are formed in the cover plate, the immunodetection reagent card further comprises a filter pad used for guiding and filtering sample solution from the sample adding hole to the liquid channel, the liquid channel is at least 1 micro-channel for the sample solution to pass through, a cross micro-array which is arranged according to a capillary force principle and used for driving the sample solution to flow in the micro-channel is arranged in the micro-channel, a first marking area, a second marking area and a third marking area are sequentially arranged in the micro-channel along the flowing direction of the sample solution, a marking antibody, a capturing antibody 1 and a capturing antibody 2 are sequentially coated in the areas, and the observation window can observe at least the second marking area and the third marking area. The method directly coats the labeled antibody and the capture antibody on the micro-channel by adopting common printing modes in the field, so that the problem that the permeability is difficult to control caused by printing the functional antibody on the gold label pad and the NC film in the prior art is avoided, the production efficiency is obviously improved, and the process complexity is reduced. As a further improvement of the utility model, the end of the microchannel is provided with a pipetting pad for absorbing sample solution passing through the microchannel. In the scheme, the liquid absorption pad is used for absorbing the residual sample solution after passing through the micro-channel, so that the residual liquid can be prevented from interfering with the detection result As a further improvement of the utility model, the cover plate is provided with the first air hole connected with the filter pad and the second air hole connected with the liquid suction pad in a penetrating way, and the air hole arrangement mode of the scheme can better realize air pressure balance in the micro-channel and optimize the flow of the sample solution in the micro-channel. The micro-channel can be formed by arranging the micro-channel groove on the bottom plate in advance, and sealing the cover plate on the top of the micro-channel groove in an ultrasonic bonding mode by the bottom plate and the cover plate, so that the micro-channel groove and the cover plate are enclosed to form the micro-channel. It will be readily appreciated that other means of pre-mounting the complete individual micro-channels may be used, as long as the arrangement of the micro-channels is accomplished. In order to ensure easy and smooth flow and proper flow rate of the sample and facilitate later observation, the width of the micro-channel is preferably 20-40 mm, and the height is preferably 30-50 μm. It is easy to understand that the novel cross microarray utilizes the characteristic that liquid spontaneously flows forward under capillary force, and the self-driven crawling capability of the liquid can be further improved after the scheme