Search

CN-224219294-U - Stem cell cryopreservation tube

CN224219294UCN 224219294 UCN224219294 UCN 224219294UCN-224219294-U

Abstract

The utility model discloses a stem cell cryopreservation tube, which belongs to the technical field of cryopreservation tubes and comprises an outer tube, a sealing cover for sealing an opening at the top of the outer tube, a movable rod connected to the bottom of the sealing cover through a connecting piece, wherein the top of the movable rod rotates around a shaft along the axis of the sealing cover, a balancing weight fixed at the bottom of the movable rod, a spiral flow channel which is spirally downward is arranged on the inner wall of the outer tube, a liquid inlet and a liquid outlet which are communicated with the top and the bottom of the spiral flow channel are respectively arranged at the top and the bottom of the outer wall of the outer tube, an inner tube is arranged at the top of the inner tube in a fitting manner, and a second limit for clamping the top of the outer tube is arranged at the top of the inner tube in a circle. The utility model provides a stem cell cryopreservation tube which accelerates the thawing of stem cells in a hand-shaking mode, and has the advantages of simple structure and low cost.

Inventors

  • WANG YIMING
  • Song Mengxiong
  • CAI JUNFENG
  • WU YUKUAN

Assignees

  • 上海市东方医院（同济大学附属东方医院）

Dates

Publication Date
20260512
Application Date
20250530

Claims (7)

  1. 1. A stem cell cryopreservation tube, comprising: an outer tube (1); A cover (3) for sealing the top opening of the outer tube (1); The movable rod (4) is connected to the bottom of the sealing cover (3) through a connecting piece, and the vertex of the movable rod (4) rotates around the axis of the sealing cover (3); the balancing weight (5) is fixed at the bottom of the movable rod (4).
  2. 2. Stem cell cryopreservation tube according to claim 1, characterized in that the connection is a nylon rope (13) or a universal joint.
  3. 3. Stem cell cryopreservation tube according to claim 2, characterized in that the cover (3) is screwed to the top of the outer tube (1).
  4. 4. The stem cell cryopreservation tube according to claim 3, wherein the inner wall of the outer tube (1) is provided with a spiral flow channel (10) which spirals downwards, the top and the bottom of the outer wall of the outer tube (1) are respectively provided with a liquid inlet (11) and a liquid outlet (12) which are communicated with the top and the bottom of the spiral flow channel (10), The novel anti-theft device is characterized by further comprising an inner tube (2), wherein the outer wall of the inner tube (2) is attached to the inner wall of the outer tube (1), and a circle of second limit (9) used for being clamped with the top of the outer tube (1) is arranged at the top of the inner tube (2).
  5. 5. The stem cell cryopreservation tube according to claim 4, wherein a gap is formed between the bottom of the inner tube (2) and the bottom of the inner wall of the outer tube (1), and the spiral flow passage (10) is communicated with the gap.
  6. 6. The stem cell cryopreservation tube according to claim 5, wherein a first limit (8) is arranged on the upper portion of the inner wall of the inner tube (2), a limit ring (6) is detachably mounted on the first limit (8), a limit round hole is formed in the middle of the limit ring (6), and the movable rod (4) penetrates through the limit round hole.
  7. 7. The stem cell cryopreservation tube according to claim 6, wherein an ultrafiltration membrane (7) is arranged at the bottom of the limiting ring (6), a circle of top opening of the ultrafiltration membrane (7) is fixed at the bottom of the limiting ring (6), the limiting round hole is communicated with the inside of the ultrafiltration membrane (7), and the balancing weight (5) is located inside the ultrafiltration membrane (7).

Description

Stem cell cryopreservation tube Technical Field The utility model relates to the technical field of cryopreservation pipes, in particular to a stem cell cryopreservation pipe. Background A cryoprotectant tube is a specialized container used in laboratories to cryopreserve biological samples. The patent of 202121014223.5 discloses a mesenchymal stem cell freezes deposits pipe, including the body, the inside rotary rod that sets up of body, set up spiral stirring piece on the rotary rod, the rotary rod lower extreme is connected with the inside rotation of body through the bearing, body top threaded connection tube cap. Above-mentioned patent is with motor drive and gear cooperation when the cell unfreezes, and the stirring piece on the drive rotary rod is to thawing with higher speed to the cell of freezing the pipe, but the structure is too complicated, still needs live working, complex operation. Disclosure of utility model The utility model provides a stem cell cryopreservation tube for overcoming the technical problems in the prior art. In order to solve the technical problems, the technical scheme of the utility model is as follows: a stem cell cryopreservation tube comprising: An outer tube; a cover for sealing the top opening of the outer tube; The movable rod is connected to the bottom of the sealing cover through a connecting piece, and the vertex of the movable rod rotates around the axis of the sealing cover; and the balancing weight is fixed at the bottom of the movable rod. In the thawing process, through rocking, can let the movable rod stir the cell sap in the outer tube, the balancing weight increases the counter weight in this in-process. Preferably, the outer tube is made of polypropylene. Preferably, the connecting piece is a nylon rope or a universal joint. Preferably, the universal joint is a hook. Preferably, the closure cap is threadably connected to the top of the outer tube. Preferably, the inner wall of the outer tube is provided with a spiral flow channel which spirals downwards, the top and the bottom of the outer wall of the outer tube are respectively provided with a liquid inlet and a liquid outlet which are communicated with the top and the bottom of the spiral flow channel, The novel inner pipe is characterized by further comprising an inner pipe, wherein the outer wall of the inner pipe is attached to the inner wall of the outer pipe, and a circle of second limit used for being clamped with the top of the outer pipe is arranged at the top of the inner pipe. Preferably, a gap is arranged between the bottom of the inner pipe and the bottom of the inner wall of the outer pipe, and the spiral flow passage is communicated with the gap. Preferably, the upper part of the inner wall of the inner pipe is provided with a first limit, a limit ring is detachably arranged on the first limit, a limit round hole is formed in the middle of the limit ring, and the movable rod penetrates through the limit round hole. Limiting round holes are formed in the middle of the limiting rings to limit the swing amplitude of the movable rod, so that the configuration blocks are prevented from colliding with the pipe wall. Preferably, the limiting ring bottom is provided with the milipore filter, and the open-top round of milipore filter is fixed in the limiting ring bottom, spacing round hole intercommunication the milipore filter is inside, and the balancing weight is located inside the milipore filter. And filtering and purifying by arranging an ultrafiltration membrane. Preferably, the ultrafiltration membrane material is an organic polymer membrane. Compared with the prior art, the technical scheme of the utility model has the beneficial effects that: The utility model provides a stem cell cryopreservation tube, wherein a movable rod is arranged in the tube and is balanced at the bottom of the tube, the movable rod is stirred in stem cell liquid in a hand-shaking mode to accelerate the thawing process, and the stem cell cryopreservation tube specifically comprises: 1) The limiting round hole in the middle of the limiting ring can reduce the swing amplitude of the movable rod, and the balancing weight is prevented from striking the pipe wall in the swinging process through reasonable size arrangement. 2) Ultrafiltration membrane which entraps macromolecular substances, colloid, particulates and bacteria on the upper part for purification. 3) If the outer tube is directly placed in freezing liquid such as liquid nitrogen, the freezing effect of stem cells in the tube is inconsistent due to insufficient liquid level, and the freezing liquid is circulated by using the spiral flow channel, so that the freezing effect of stem cells in all positions in the tube is consistent. Drawings In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly desc