CN-224221386-U - Magnetic attraction heating device and biological detection equipment

Abstract

The application relates to the technical field of high-flux sequencing, in particular to a magnetic heating device and biological detection equipment, wherein the magnetic heating device comprises a heat conduction piece, a plurality of heating positions are arranged on the upper surface of the heat conduction piece, the plurality of heating positions are used for placing a reaction container, and installation gaps are arranged between at least part of the heating positions; the magnetic attraction piece is arranged in the installation gap and used for adsorbing magnetic beads in the reaction container, and the shell is covered on the outer side of the heat conduction piece and is provided with an opening, and a plurality of heating positions are exposed from the opening. The magnetic heating device provided by the application is beneficial to improving the hybridization efficiency of a sequencing library.

Inventors

• WEI FANCHENG
• WU XIAOHUI
• LI YONGHUI
• XUE CHENQUAN
• SHU GAOFENG
• NIE MENGQING

Assignees

• 上海小荷医学检验实验室有限公司

Dates

Publication Date

20260512

Application Date

20250514

Claims (16)

1. 1. A magnetic heating device for a biological detection apparatus, comprising: The upper surface of the heat conduction piece is provided with a plurality of heating positions, the heating positions are used for placing the reaction container, and installation gaps are formed between at least part of the heating positions; The heat source piece is in heat conduction connection with the heat conduction piece and heats the reaction container through the heat conduction piece; the magnetic attraction piece is arranged in the installation gap and is used for adsorbing magnetic beads in the reaction container; and a housing covering the heat conductive member, wherein the housing has an opening, and a plurality of heating sites are exposed from the opening.
2. 2. The magnetically attractable heating device of claim 1 wherein the thermally conductive member includes: A base portion to which the housing is connected; And the heat conducting part is arranged on the upper side of the base body part and extends out of the opening, and the heating positions are all formed on the upper surface of the heat conducting part.
3. 3. The magnetic attraction heating device according to claim 2, wherein the heat conducting part is a plurality of heat conductors distributed at intervals along a first direction, and a plurality of heating positions distributed along a second direction are arranged on each heat conductor; The mounting gaps extending in the second direction are defined between two adjacent heat conductors.
4. 4. A magnetically attractable heating arrangement according to claim 3 wherein the height of the magnetically attractable member is greater than the height of the mounting gap.
5. 5. The magnetic attraction heating device according to claim 4, wherein a plurality of fixing structures are arranged on the shell, the fixing structures are arranged on the periphery of the opening, and the fixing structures are used for fixing the magnetic attraction piece.
6. 6. The magnetic attraction heating device according to claim 5, wherein the fixing structure is provided at both side edges of the opening in the second direction, The fixing structure is formed as a pressing cantilever, is positioned on the upper side of the installation gap and is in pressing fit with the upper surface of the magnetic attraction piece.
7. 7. A magnetically attractable heating arrangement according to claim 1 wherein the magnetically attractable member is adapted to move within the mounting gap between a first position in which the beads within the reaction vessel are subject to the magnetic attraction of the magnetically attractable member and a second position in which the beads within the reaction vessel are free of the magnetic attraction of the magnetically attractable member.
8. 8. The magnetic attraction heating device according to claim 1, wherein the magnetic attraction member is an electromagnetic member to control a magnetic attraction force of the magnetic attraction member to magnetic beads in the reaction vessel by energization and de-energization of the magnetic attraction member.
9. 9. A magnetically attractable heating device according to any one of claims 2-8 wherein the housing is covered on an upper surface of the base portion and at least part of the side surface of the base portion.
10. 10. The magnetically attractable heating device of claim 9 wherein the base portion includes: A first base portion; A second base portion provided on an upper side of the first base portion, and a projection of the second base portion in a reference plane is located inside a projection of the first base portion in the reference plane so that a first step structure is formed between the first base portion and the second base portion, and a projection of a heat conduction portion in the reference plane is located inside a projection of the second base portion in the reference plane so that a second step structure is formed between the heat conduction portion and the second base portion; The housing has a first support structure and a second support structure, the first support structure being supported by the first step structure, the second support structure being supported by the second step structure.
11. 11. The magnetic attraction heating device according to claim 10, wherein a plurality of hollow structures are arranged on the second base body part at intervals, and the heat conducting part is positioned at the joint of the hollow structures.
12. 12. A magnetically attractable heating device according to any one of claims 1 to 8 wherein the heat source member is provided on a side of the heat conducting member opposite the heating location.
13. 13. A magnetically attractable heating device according to any one of claims 1-8 wherein the heat source includes: A heating part; a temperature detection unit connected to the heat generating portion to detect a temperature of the heat generating portion; And the temperature control unit is respectively connected with the heating part and the temperature detection unit and is used for regulating and controlling the temperature of the heating part.
14. 14. The magnetic attraction heating device of claim 13, wherein the heat generating part is at least one of a semiconductor heating and cooling sheet, an electric heating element, a ceramic heater, an electrothermal film, and an electrothermal wire.
15. 15. A magnetically attractable heating arrangement according to any one of claims 1-8 wherein the housing is an insulating material.
16. 16. A biological detection apparatus comprising the magnetic heating device according to any one of claims 1 to 15.

Description

Magnetic attraction heating device and biological detection equipment Technical Field The application relates to the technical field of high-flux sequencing, in particular to a magnetic heating device and biological detection equipment. Background With the development of high throughput sequencing (Next-Generation Sequencing, NGS) technology, the method has been increasingly used in the fields of early screening of tumors, concomitant diagnosis, infectious diseases and the like. The NGS technology can perform high-throughput and high-depth detection on the genome in a short time, and can realize early detection, accurate diagnosis and treatment of diseases. NGS techniques include library amplification and purification, probe hybridization, capture, elution, and library quality inspection. Among them, the probe hybridization process and the probe capture process are critical to the accuracy and reliability of the experimental results. The probe hybridization process requires control of the reaction temperature. Probe capture requires washing of the beads to remove impurities and restore activity. In the existing NGS technology, the reaction vessel is heated by a heating module to complete the probe hybridization process. And performing magnetic attraction operation on the reaction container through a magnetic attraction module so as to finish the probe capturing process. However, after the reaction vessel is transferred from the heating module to the magnetic attraction module, the temperature in the reaction vessel is rapidly reduced, so that the hybridization reaction of the sequencing library is inhibited, and the hybridization efficiency of the sequencing library is reduced. Disclosure of utility model The magnetic heating device and the biological detection equipment provided by the application are beneficial to improving the hybridization efficiency of a sequencing library. The application provides a magnetic attraction heating device which is used for biological detection equipment and comprises a heat conduction piece, a heat source piece, a magnetic attraction piece and a shell, wherein the upper surface of the heat conduction piece is provided with a plurality of heating positions, the heating positions are used for placing a reaction container, an installation gap is arranged between at least part of the heating positions, the heat source piece is in heat conduction connection with the heat conduction piece and is used for heating the reaction container through the heat conduction piece, the magnetic attraction piece is arranged in the installation gap and is used for adsorbing magnetic beads in the reaction container, the shell is covered on the outer side of the heat conduction piece, and the shell is provided with an opening, and a plurality of heating positions are exposed from the opening. In one possible implementation mode, the heat conducting piece comprises a base body part, a heat conducting part and a heating position, wherein the base body part is connected with the shell, the heat conducting part is arranged on the upper side of the base body part and extends out of the opening, and the heating positions are formed on the upper surface of the heat conducting part. In one possible implementation manner, the heat conducting part is a plurality of heat conducting bodies distributed at intervals along a first direction, a plurality of heating positions distributed along a second direction are arranged on each heat conducting body, and the mounting gaps extending along the second direction are defined between two adjacent heat conducting bodies. In one possible embodiment, the height of the magnetic attraction element is higher than the height of the mounting gap. In one possible implementation manner, the shell is provided with a plurality of fixing structures, the fixing structures are arranged on the periphery of the opening, and the fixing structures are used for fixing the magnetic attraction piece. In one possible implementation manner, the fixing structure is arranged at two side edges of the opening along the second direction, the fixing structure is formed into a pressing cantilever, and the fixing structure is arranged at the upper side of the mounting gap and is in pressing fit with the upper surface of the magnetic attraction piece. In a possible embodiment, in the mounting gap, the magnetic attraction member is adapted to move between a first position in which the magnetic beads in the reaction vessel are subjected to the magnetic attraction force of the magnetic attraction member and a second position in which the magnetic beads in the reaction vessel are separated from the magnetic attraction force of the magnetic attraction member. In one possible embodiment, the magnetic attraction member is an electromagnetic member to control the magnetic attraction force of the magnetic attraction member to the magnetic beads in the reaction vessel by energizing and de-energizing the ma