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CN-224226928-U - Polypeptide schizolysis precipitation tank

CN224226928UCN 224226928 UCN224226928 UCN 224226928UCN-224226928-U

Abstract

The utility model relates to the technical field of precipitation tanks, in particular to a polypeptide pyrolysis precipitation tank. The technical scheme includes that the novel precipitation tank mainly aims at solving the problems that when an existing precipitation tank discharges through a bottom discharge pipe after resin precipitation, upper-layer suspended matters are easy to carry, the purity of a target peptide chain is reduced, subsequent purification is difficult, cost is increased, and production quality requirements are difficult to meet. The utility model can improve the purity of the target peptide chain, reduce the subsequent purification difficulty and cost, ensure the quality stability of the polypeptide product, meet strict production quality standards and improve the production efficiency and economic benefit of polypeptide synthesis.

Inventors

  • TAO LIMING
  • Xu Lvzhong

Assignees

  • 上海昱郦生物科技有限公司

Dates

Publication Date
20260512
Application Date
20250605

Claims (10)

  1. 1. A polypeptide lysis precipitation tank, comprising: The cooling device comprises a tank body (1), wherein a cooling sleeve (2) is sleeved on the outer side of the tank body (1); The stirring assembly (3) is arranged on the tank body (1), and the stirring assembly (3) is used for stirring the resin in the tank body (1); The floating removing mechanism (4) is arranged on the tank body (1) and the stirring assembly (3), and the floating removing mechanism (4) is used for sucking the suspension liquid after resin precipitation in the tank body (1); Install in inlet pipe (5) at jar body (1) top, jar body (1) top still is connected with shower (6), shower ball (61) are installed to shower (6) bottom.
  2. 2. The polypeptide pyrolysis precipitation tank according to claim 1, wherein the stirring assembly (3) comprises a rotating sleeve (31) which is connected to the top of the tank body (1) in a rotating mode, a driving rod (32) is connected in a sliding mode in the rotating sleeve (31), the driving rod (32) is arranged in a regular quadrangular mode, a stirring rod (33) is fixedly connected to the bottom of the driving rod (32), a plurality of groups of stirring blades (34) are arranged on the periphery of the stirring rod (33), a positioning ring (35) is arranged below the stirring rod (33), the stirring rod (33) is connected in the positioning ring (35) in a sliding mode, and a plurality of groups of fixing rods (36) are fixedly connected between the positioning ring (35) and the tank body (1).
  3. 3. The polypeptide pyrolysis precipitation tank according to claim 2, wherein the stirring assembly (3) further comprises a mounting frame (37) fixedly connected to the top of the tank body (1), the mounting frame (37) is in a '冂' shape, a servo motor (38) is mounted on the top of the mounting frame (37), an output end of the servo motor (38) penetrates through the mounting frame (37) and is fixedly connected with a first gear (39), a second gear (310) meshed with the first gear (39) is arranged on one side of the first gear, and the second gear (310) is fixedly connected to the outer ring of the rotating sleeve (31).
  4. 4. A polypeptide cracking precipitation tank according to claim 3, wherein the floating removing mechanism (4) comprises a positioning sleeve (41) fixedly connected to the top of the driving rod (32), a first moving plate (42) is rotatably connected to the outer ring of the positioning sleeve (41), two groups of first push rod motors (43) are mounted on the top of the tank body (1), and the output ends of the two groups of first push rod motors (43) are fixedly connected with the first moving plate (42).
  5. 5. The polypeptide pyrolysis precipitation tank according to claim 4, wherein the floating removing mechanism (4) further comprises a connecting rod (44) fixedly connected to the outer ring of the stirring rod (33), the connecting rod (44) is arranged below the plurality of groups of stirring blades (34), the driving rod (32), the stirring rod (33) and the connecting rod (44) are provided with liquid discharging channels (45) in a sharing mode, the bottom of the connecting rod (44) is provided with a plurality of groups of through holes (46) communicated with the liquid discharging channels (45), the top of the driving rod (32) is provided with a connecting sleeve (47), a connecting pipe (48) is arranged above the connecting sleeve (47), a rubber ring is arranged at the bottom of the connecting pipe (48), the top of the connecting pipe (48) is connected with a micropump (49) through a pipeline, and the output end of the micropump (49) is connected with a sewage draining pipe.
  6. 6. The polypeptide pyrolysis precipitation tank according to claim 5, wherein the floating removing mechanism (4) further comprises a second moving plate (410) fixedly connected to an outer ring of the connecting pipe (48), two groups of second push rod motors (411) are installed at the top of the tank body (1), the output ends of the two groups of second push rod motors (411) penetrate through the second moving plate (410) and are fixedly connected with a limiting plate (412), a spring (413) is sleeved at the output end of the second push rod motor (411), the spring (413) is located between the second moving plate (410) and the limiting plate (412), a supporting ring (414) is sleeved at the output end of the second push rod motor (411), and the supporting ring (414) is located below the second moving plate (410).
  7. 7. The polypeptide pyrolysis precipitation tank according to claim 6, wherein a discharge pipe (11) is connected to the bottom of the tank body (1), and an electromagnetic valve (12) is installed on the tank body (1).
  8. 8. The polypeptide pyrolysis precipitation tank according to claim 7, wherein the two sides of the cooling sleeve (2) are respectively connected with a liquid inlet pipe (21) and a liquid outlet pipe (22), and the liquid inlet pipe (21) is positioned above the liquid inlet pipe (21).
  9. 9. The polypeptide pyrolysis precipitation tank according to claim 8, wherein an eyepiece (7) is mounted on top of the tank body (1).
  10. 10. The polypeptide lysis and precipitation tank according to claim 7, further comprising a control box, wherein the electromagnetic valve (12), the servo motor (38), the first push rod motor (43), the micropump (49) and the second push rod motor (411) are electrically connected with the control box.

Description

Polypeptide schizolysis precipitation tank Technical Field The utility model relates to the technical field of precipitation tanks, in particular to a polypeptide pyrolysis precipitation tank. Background Polypeptides are biologically active substances which are widely involved in various cellular functions in organisms, and whose molecular structure is between amino acids and proteins, and which are formed by binding a plurality of amino acids through peptide bonds according to a specific arrangement sequence. In the synthetic flow of polypeptide, firstly, the hydroxyl group of the amino acid at the tail end of the target peptide chain is connected to insoluble high polymer resin in a covalent bond form, the amino acid on a solid phase carrier is used as an amino component, after removing amino protecting groups, the amino protecting groups react with excessive activated hydroxyl components to connect a long peptide chain, and the target peptide chain length is achieved by continuously repeating the operations of condensation, washing, deprotection, neutralization and washing-next round condensation. The cleavage of peptide chains from the resin is a key step in the synthesis process, in which the precipitation step of the resin is particularly important and needs to be performed in a precipitation tank, which directly affects the purity and efficiency of the subsequent separation of peptide chains. Currently, existing settling tanks typically drain the precipitated fraction through a drain pipe at the bottom after resin precipitation is completed. However, this conventional discharge has a significant disadvantage in that a large amount of the upper suspended matters are inevitably carried along when the bottom sediment is discharged, particularly near the end of the last partial sediment discharge. The mixing of these suspensions not only reduces the purity of the target peptide chain, which results in great increase in the processing difficulty and cost increase in the subsequent purification process, but also affects the quality stability of the final polypeptide product, and makes it difficult to meet the increasingly severe production and quality standards. In view of the above, the utility model provides a polypeptide cracking precipitation tank. Disclosure of utility model The utility model aims to solve the problems that in the prior art, when the existing precipitation tank discharges materials through a bottom discharge pipe after resin precipitation, upper suspended matters are easy to carry, so that the purity of a target peptide chain is reduced, the subsequent purification is difficult, the cost is increased, and the production quality requirement is difficult to meet. The technical scheme includes that the polypeptide pyrolysis precipitation tank comprises a tank body, a stirring assembly, a floating removing mechanism and a feeding pipe, wherein a cooling sleeve is sleeved on the outer side of the tank body, the stirring assembly is arranged on the tank body and used for stirring resin in the tank body, the floating removing mechanism is arranged on the tank body and the stirring assembly and used for sucking suspension liquid after resin precipitation in the tank body, the feeding pipe is arranged at the top of the tank body, the top of the tank body is further connected with a spray pipe, and a spray ball is arranged at the bottom of the spray pipe. Optionally, the stirring subassembly is including rotating the cover of connecting in jar body top, sliding connection has the actuating lever in the cover of rotating, the actuating lever is regular quadrangular prism shape setting, actuating lever bottom fixedly connected with puddler, multiunit stirring leaf is installed to the puddler periphery, the puddler below is provided with the holding ring, puddler sliding connection is in the holding ring, fixedly connected with multiunit dead lever between holding ring and the jar body. Optionally, the stirring subassembly still includes fixed connection in the installing frame at jar body top, the installing frame is "冂" font setting, servo motor is installed at the installing frame top, servo motor's output runs through the installing frame and fixedly connected with first gear, first gear one side is provided with rather than the second gear of meshing, second gear fixed connection is at the rotation cover outer lane. Optionally, the floating liquid removing mechanism comprises a positioning sleeve fixedly connected to the top of the driving rod, a first moving plate is rotationally connected to the outer ring of the positioning sleeve, two groups of first push rod motors are mounted on the top of the tank body, and the output ends of the two groups of first push rod motors are fixedly connected with the first moving plate. Optionally, remove floating liquid mechanism still includes fixed connection in the connecting rod of puddler outer lane, the connecting rod sets up in multiunit stirring le