Search

CN-224226995-U - Plasmid cracker with nine-way valve

CN224226995UCN 224226995 UCN224226995 UCN 224226995UCN-224226995-U

Abstract

The utility model discloses a plasmid pyrolysis device provided with a nine-way valve, which comprises a liquid adding mechanism, a separation mixing mechanism and a pyrolysis mechanism, wherein the pyrolysis mechanism comprises a pyrolysis part for providing a place where plasmids and pyrolysis liquid are subjected to pyrolysis and a switching part communicated with the pyrolysis part, a neutralization mechanism and a collection mechanism, the pyrolysis mechanism is used for connecting the liquid adding mechanism and the neutralization mechanism, the separation mixing mechanism is positioned between the liquid adding mechanism and the pyrolysis mechanism, the pyrolysis part comprises a first pyrolysis part, a second pyrolysis part, a third pyrolysis part and a fourth pyrolysis part, the switching part is the nine-way valve, and independent pyrolysis or serial pyrolysis among the pyrolysis parts is realized by switching the nine-way valve. Compared with the prior art, the utility model can realize the series connection of the cracking parts by only one nine-way valve, obtain the cracking parts with different lengths, obtain different cracking time lengths, avoid reconnecting the length of the cracking parts and improve the screening time.

Inventors

  • NI HAIJUN

Assignees

  • 苏州博腾生物制药有限公司

Dates

Publication Date
20260512
Application Date
20250329

Claims (10)

  1. 1. A plasmid cracker with nine-way valve is characterized by comprising, A liquid adding mechanism (10) for adding bacterial suspension and lysate into the plasmid cracker; a separation and mixing mechanism (11) for separating plasmids in the initial bacterial suspension and mixing plasmids with the lysate; A lysis mechanism (12) comprising a lysis section for providing a place where the plasmid and the lysis solution are lysed, and a switching section in communication with the lysis section; A neutralization mechanism (13) for adding a neutralization solution to the mixture after the pyrolysis; a collection mechanism (14) for collecting the neutralized mixed liquid; the cracking mechanism (12) connects the liquid adding mechanism (10) with the neutralizing mechanism (13); the separation and mixing mechanism (11) is positioned between the liquid adding mechanism (10) and the cracking mechanism (12); The cracking part comprises a first cracking part (121), a second cracking part (122), a third cracking part (123) and a fourth cracking part (124), the switching part is a nine-way valve, and independent cracking or series-connection post-cracking among the cracking parts is realized by switching the nine-way valve.
  2. 2. The plasmid cracker with nine-way valve according to claim 1, comprising a valve body (15), a valve core (16) rotatable in the valve body (15), and an end cover (17) for sealing the valve core (16) and fixed on the valve body (15), wherein nine pipe orifices are arranged on the valve body (15), the valve core (16) is a cylinder, three passages are arranged on the upper surface of the valve core (16), one end ports of the two passages are converged into one port, two passages are arranged on the lower surface of the valve core (16), and each passage on the valve core (16) can be communicated with the two pipe orifices on the valve body (15).
  3. 3. The plasmid cracker with nine-way valve according to claim 2, wherein the nine nozzles on the valve body (15) are a first nozzle (1), a second nozzle (2), a third nozzle (3), a fourth nozzle (4), a fifth nozzle (5), a sixth nozzle (6), a seventh nozzle (7), an eighth nozzle (8) and a ninth nozzle (9), respectively.
  4. 4. A plasmid cracker with nine-way valve according to claim 3, wherein the three passages on the upper surface of the valve core (16) are a-c passage, m-c passage, h-e passage, and the two passages on the lower surface of the valve core (16) are f-k passage, b-d passage.
  5. 5. The plasmid cracker with nine-way valve according to claim 4, wherein the first cracker (121) is connected to the first orifice (1), the second cracker (122) is connected between the third orifice (3) and the fifth orifice (5), and the third cracker (123) is connected in series with the fourth connector and is connected between the eighth orifice (8) and the ninth orifice (9).
  6. 6. The plasmid cracker with nine-way valve according to claim 5, wherein the second orifice (2) is directly connected to the fourth orifice (4), which after connection is led into the outlet line, and the sixth orifice (6) is directly connected to the seventh orifice (7).
  7. 7. The plasmid cracker with nine-way valve according to claim 6, wherein the first cracker (121) is operated with one end of the m-c channel being in communication with the first orifice (1) and the other end being in communication with the fourth orifice (4) when the nine-way valve is in the initial state.
  8. 8. The plasmid cracker with nine-way valve according to claim 7, wherein the valve core (16) of the nine-way valve is rotated so that one end of the a-c path is communicated with the first nozzle (1) and the other end is communicated with the third nozzle (3), one section of the b-d path is communicated with the fifth nozzle (5) and the other end is communicated with the second nozzle (2), and the first cracker (121) and the second cracker (122) work together.
  9. 9. A plasmid cracker with nine-way valve according to claim 8, wherein the valve core (16) of the nine-way valve is rotated such that the h-e passage has one end connected to the first nozzle (1) and the other end connected to the eighth nozzle (8), the f-k passage has one end connected to the ninth nozzle (9) and the other end connected to the second nozzle (2), and the first cracker (121), the third cracker (123) and the fourth cracker (124) work together.
  10. 10. A plasmid cracker fitted with a nine-way valve according to claim 9, wherein the nine-way valve cartridge (16) is rotated such that the f-k passage is in communication with the first orifice (1) at one end, the eighth orifice (8) at the other end, the h-e passage is in communication with the ninth port at one end, the seventh port at the other end, the sixth orifice (6) at one end, the third orifice (3) at the other end, the fifth orifice (5) at one end, the second orifice (2) at the other end, the first (121), the second (122), the third (123) and the fourth (124) cracking sections work together.

Description

Plasmid cracker with nine-way valve Technical Field The utility model relates to the technical field of biology, in particular to a plasmid cracking device with a nine-way valve. Background With the accelerated development of cell and gene therapy (CELL AND GENE THERAPY), the most commonly used vectors in the cell and gene therapy fields, AAV (adenovirus) and LV (lentivirus), are produced by taking plasmids as important production raw materials. The plasmid development process flow comprises strain construction, fermentation culture, cracking clarification, concentration replacement and chromatography purification. Wherein the cleavage of the plasmid is effected by a variety of factors in the process to obtain various forms of plasmid, supercoiled plasmid DNA (scDNA), open-loop DNA (ocDNA), linear DNA, aggregates of plasmid DNA, and the like. In addition, impurities such as host proteins (HCPs), host nucleic acids (HCDs), RNAs, endotoxins, and the like are accompanied. Insufficient lysis can reduce the recovery rate of plasmids, excessive lysis can destroy plasmid molecules, so that feed liquid is viscous and difficult to filter, and bacterial cell walls can be largely cracked into soluble endotoxin, so that the pressure of subsequent purification is increased. Therefore, precise control of the lysis parameters is required, and timely separation of the released plasmid from the bacterial debris is required. Before the plasmid is put into production, technological conditions are repeatedly searched, and the efficiency of the screening process can be greatly improved by instruments and equipment suitable for small-batch screening in a laboratory. Six-way valves and three-way valves which are commonly used in the existing instrument are inconvenient to control because a plurality of valves are needed to be added under various screening conditions. Disclosure of utility model In order to achieve the above-mentioned aim, the utility model discloses a plasmid cracker provided with a nine-way valve, which uses the nine-way valve to communicate 4 cracking parts, so that various communication modes can be realized, different pipeline lengths of cracking sites are obtained, and further different cracking times are obtained, the problem that different pipelines are required to be installed when a laboratory screens the plasmid cracking time is solved, the screening efficiency is improved, the size of the device is reduced, and the defect that the cracking time has errors due to the operation of a plurality of valve bodies is overcome. The utility model discloses a plasmid cracker provided with a nine-way valve, which comprises, The liquid adding mechanism is used for adding bacterial suspension and lysate into the plasmid cracking device; The separation and mixing mechanism is used for separating plasmids in the initial bacterial suspension and mixing plasmids with the lysate; the cracking mechanism comprises a cracking part for providing a place where the plasmid and the cracking liquid are cracked, and a switching part communicated with the cracking part; The neutralization mechanism is used for adding neutralization solution into the cracked mixed solution; the collecting mechanism is used for collecting the neutralized mixed liquid; The cracking mechanism connects the liquid adding mechanism with the neutralizing mechanism; the separation and mixing mechanism is positioned between the liquid adding mechanism and the cracking mechanism; The cracking part comprises a first cracking part, a second cracking part, a third cracking part and a fourth cracking part, the switching part is a nine-way valve, and independent cracking or serial cracking among the cracking parts is realized by switching the nine-way valve. Compared with the prior art, the utility model can realize the series connection of the cracking parts by only one nine-way valve, obtain the cracking parts with different lengths, obtain different cracking time lengths, avoid reconnecting the length of the cracking parts and improve the screening time. Drawings FIG. 1 is a schematic diagram of the structure of the present utility model; FIG. 2 is a schematic diagram of the nine-way valve of the present utility model; FIG. 3 is a schematic illustration of the first cracking unit of the present utility model in operation; FIG. 4 is a schematic diagram of the first and second cracking sections of the present utility model in operation; FIG. 5 is a schematic diagram showing the communication of the first cracking unit, the third cracking unit and the fourth cracking unit in operation; FIG. 6 is a schematic diagram showing the communication of the first, second, third and fourth cracking units of the present utility model when they are operated. In the figure, a first nozzle 1, a second nozzle 2, a third nozzle 3, a fourth nozzle 4, a fifth nozzle 5, a sixth nozzle 6, a seventh nozzle 7, an eighth nozzle 8, a ninth nozzle 9, a liquid adding mechanism 10, a separa