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CN-224226999-U - Centrifuge tube rack for preventing organoid matrigel from solidifying

CN224226999UCN 224226999 UCN224226999 UCN 224226999UCN-224226999-U

Abstract

The utility model provides a centrifuge tube rack for preventing organoid matrigel from solidifying, which comprises an upper support piece and a lower support piece which are arranged at intervals along the vertical direction, wherein the upper support piece is provided with a plurality of tube holes, the tube holes are distributed at intervals along the horizontal direction, the centrifuge tube rack also comprises a containing piece, a containing area for low-temperature medium is arranged in the containing piece, the upper end of the containing piece is provided with an opening communicated with the containing area, and the opening corresponds to the tube holes. This prevent centrifuge tube rack that organoid matrigel solidifies can be with holding the piece and move to the outside of upper support piece and lower support piece through import and export, places low temperature medium in holding the region to will hold the piece and reset, when using, insert the centrifuging tube in holding the region through tube hole and opening, realize low temperature medium effect centrifuging tube, avoid centrifuging tube internal matrigel and matrigel organoid mixture to solidify.

Inventors

  • ZHANG YUHAN
  • SHI CHAOJI
  • DENG XIAOLING
  • ZHANG ZHIYUAN

Assignees

  • 上海交通大学医学院附属第九人民医院

Dates

Publication Date
20260512
Application Date
20250509

Claims (10)

  1. 1. The utility model provides a prevent organ-like matrigel solidification's centrifuge tube rack, includes upper support piece (100) and lower support piece (200) that follow vertical direction interval arrangement, last tube hole (101) have been seted up on support piece (100), the quantity of tube hole (101) is a plurality of, tube hole (101) are along horizontal direction interval distribution, a serial communication port, centrifuge tube rack still includes: The device comprises a holding piece (300), wherein a holding area (300 a) for low-temperature medium is arranged in the holding piece (300), an opening (301) communicated with the holding area (300 a) is formed in the upper end of the holding piece (300), the opening (301) corresponds to the pipe hole (101), the holding piece (300) is movably arranged between the upper supporting piece (100) and the lower supporting piece (200), and an inlet and outlet (501) for the holding piece (300) to enter and exit is formed in one side of the upper supporting piece (100) and one side of the lower supporting piece (200) in the horizontal direction; Be provided with iris mechanism (400) on tube hole (101), iris mechanism (400) are including a plurality of edges circumference of tube hole (101) interval arrangement's blade (401) in proper order, blade (401) are with following radial mode activity of tube hole (101), wherein the inboard of blade (401) encloses into a size adjustable centrifuging tube and places regional (401 a), the inboard of blade (401) is provided with flexible water guide piece (402), water guide piece (402) are set up the outer wall that is suitable for the centrifuging tube of laminating.
  2. 2. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, wherein one iris mechanism (400) is provided on each tube hole (101).
  3. 3. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, characterized in that the iris mechanism (400) further comprises a driving element (403), the driving element (403) is drivingly connected to the blade (401), between the driving element (403) and the tube hole (101), and/or between the blade (401) and the tube hole (101) an elastic element (404) is provided, the elastic element (404) being arranged to provide the blade (401) with a force moving radially inwards towards the tube hole (101).
  4. 4. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, characterized in that the water guide (402) is a sponge or a wiper strip.
  5. 5. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 4, wherein the height of the water guide (402) near the radially inner side of the tube hole (101) is higher than the height of the water guide (402) near the radially outer side of the tube hole (101), and the thickness of the water guide (402) near the radially inner side of the tube hole (101) is smaller than the thickness of the water guide (402) near the radially outer side of the tube hole (101).
  6. 6. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, characterized in that the outer edge of the opening (301) on the holding member (300) is provided with a sealing strip (3011), the sealing strip (3011) being adapted to be sealingly attached to the lower end surface of the upper support member (100) when the holding member (300) is located between the upper support member (100) and the lower support member (200).
  7. 7. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, wherein the outer surface of the holding member (300), the outer surface of the upper support member (100) and the outer surface of the lower support member (200) are covered with a sealing and insulating layer.
  8. 8. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, characterized in that a side plate (500) is provided between the outer circumference of the upper support (100) and the outer circumference of the lower support (200), the side plate (500) closes the containing member (300) between the upper support (100) and the lower support (200), the opening (301) is provided on the side plate (500), and the outer side surface of the containing member (300) in the moving direction closes the inlet/outlet (501).
  9. 9. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, wherein the low-temperature medium is ice cubes, the bottom of the containing member (300) is provided with a water outlet (302) which can be opened and closed, and the height of the bottom surface of the inner side of the containing member (300) is gradually reduced in a manner of approaching to the water outlet (302).
  10. 10. The centrifuge tube rack for preventing coagulation of organoid matrigel according to claim 1, wherein a horizontally arranged supporting plate (303) is arranged on the bottom surface of the inner side of the holding member (300), supporting holes (3031) are formed in the supporting plate (303), and the supporting holes (3031) are in one-to-one correspondence with the tube holes (101).

Description

Centrifuge tube rack for preventing organoid matrigel from solidifying Technical Field The utility model relates to the technical field of centrifuge tube racks, in particular to a centrifuge tube rack for preventing organoid matrigel from solidifying. Background Organoids are micro-organ-like structures formed by self-assembly under in vitro three-dimensional culture conditions, the culture of organoids relies on Matrigel, which provides structural scaffolds, mechanical properties, etc. for organoids, currently most commonly used Matrigel is mainly Matrigel (basement membrane extract), which is required to be operated on ice to prevent Matrigel from solidifying when operated using Matrigel, and currently is operated in laboratory, mainly using foam cartridges to carry ice cubes in a sterile operating table. The above prior art has the following drawbacks: The foam box bears the ice-cube comparatively clumsily, lacks simple and convenient test-tube rack that can bear the ice-cube and is used for placing matrigel and matrigel organoid mixture, is unfavorable for fixed centrifuging tube and operates, has influenced laboratory personnel's work efficiency, is taking out the centrifuging tube from the foam box in-process, and the ice-water whereabouts that the centrifuging tube outside is stained with and is attached easily causes the pollution to the operation panel. Therefore, in order to solve the problems, the utility model provides the centrifuge tube rack which is convenient to operate and can prevent the coagulation of the organoid matrigel and avoid the draining pollution of the operation table. Disclosure of utility model In order to solve the problems in the prior art, the utility model provides a centrifuge tube rack for preventing organoid matrigel from solidifying. According to an object of the present utility model, there is provided a centrifuge tube rack for preventing coagulation of organoid matrigel, comprising an upper support member and a lower support member arranged at intervals in a vertical direction, wherein the upper support member is provided with a plurality of tube holes, the tube holes are distributed at intervals in a horizontal direction, and the centrifuge tube rack further comprises: the device comprises a holding piece, wherein a holding area of a low-temperature medium is arranged in the holding piece, an opening communicated with the holding area is formed in the upper end of the holding piece, the opening corresponds to the pipe hole, the holding piece is movably arranged between an upper supporting piece and a lower supporting piece, and an inlet and an outlet for the holding piece to enter and exit are formed in one side of the upper supporting piece and one side of the lower supporting piece in the horizontal direction; Be provided with iris mechanism on the tube hole, iris mechanism includes a plurality of edges along the circumference of tube hole is interval arrangement's blade in proper order, the blade is with following radial mode activity of tube hole, wherein the inboard of blade encloses into a size adjustable centrifuging tube and places the region, the inboard of blade is provided with flexible water guide, the water guide is set up and is suitable for the outer wall of laminating centrifuging tube. Preferably, one iris mechanism is arranged on each tube hole. Preferably, the iris mechanism further comprises a driving element, the driving element is in driving connection with the blade, and an elastic element is arranged between the driving element and the pipe hole and/or between the blade and the pipe hole, and the elastic element is arranged and suitable for providing a force for the blade to move towards the radial inner side of the pipe hole. Preferably, the water guide member is a sponge or a wiper strip. Preferably, the height of the water guide close to the radial inner side of the pipe hole is higher than the height of the water guide close to the radial outer side of the pipe hole, and the thickness of the water guide close to the radial inner side of the pipe hole is smaller than the thickness of the water guide close to the radial outer side of the pipe hole. Preferably, a sealing strip is arranged at the outer edge of the opening on the containing piece, and the sealing strip is suitable for being in sealing fit with the lower end face of the upper supporting piece when the containing piece is positioned between the upper supporting piece and the lower supporting piece. Preferably, the outer surface of the holding part, the outer surface of the upper supporting part and the outer surface of the lower supporting part are all covered with a sealing heat-insulating layer. Preferably, a side plate is arranged between the periphery of the upper support member and the periphery of the lower support member, the side plate seals the containing member between the upper support member and the lower support member, the opening is arranged on the side plate, a