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CN-224227001-U - Deep hole plate suitable for high flux extraction of DNA of large-volume seed

CN224227001UCN 224227001 UCN224227001 UCN 224227001UCN-224227001-U

Abstract

The utility model relates to the technical field of detection equipment, in particular to a deep hole plate suitable for high-flux extraction of DNA of large-volume seeds, which is provided with a plurality of first specification holes and a plurality of second specification holes at intervals alternately arranged on the deep hole plate, the diameter of the first specification hole is larger than that of the second specification hole, the first specification hole is used for accommodating large-volume seeds, and the deep hole plate suitable for high-flux DNA extraction of large-volume seeds provided by the embodiment of the utility model can meet the requirement of large-volume seed treatment and can keep compatibility with the existing automatic equipment.

Inventors

  • WANG FENGGE
  • GE JIANRONG
  • ZHANG JIANGBIN
  • ZHANG MINGQI

Assignees

  • 北京市农林科学院

Dates

Publication Date
20260512
Application Date
20250512

Claims (10)

  1. 1. The deep-hole plate suitable for high-throughput extraction of DNA of large-volume seeds is characterized in that a plurality of first specification holes (11) and a plurality of second specification holes (12) are formed in the deep-hole plate (1), the first specification holes (11) and the second specification holes (12) are alternately arranged at intervals on the deep-hole plate (1), the diameter of the first specification holes (11) is larger than that of the second specification holes (12), and the first specification holes (11) are used for accommodating large-volume seeds.
  2. 2. Deep-well plate according to claim 1, characterized in that the deep-well plate (1) is provided with (48) first gauge holes (11) and (48) second gauge holes (12).
  3. 3. The deep-well plate of claim 2, wherein a center-to-center spacing of adjacent first gauge holes (11) and second gauge holes (12) matches a gun head spacing of a (96) channel high-throughput workstation.
  4. 4. The deep-well plate according to claim 2, characterized in that the first gauge holes (11) and the second gauge holes (12) are arranged in 8 rows and 12 columns or 12 rows and 8 columns.
  5. 5. Deep-well plate according to claim 1, characterized in that the deep-well plate (1) comprises a first deep-well plate (13) and a second deep-well plate (14) of identical structure, wherein a first gauge hole (11) on the first deep-well plate (13) corresponds to a second gauge hole (12) of the second deep-well plate (14) in position, and a second gauge hole (12) on the first deep-well plate (13) corresponds to a first gauge hole (11) on the second deep-well plate (14) in position.
  6. 6. Deep-well plate according to claim 1, characterized in that the first gauge hole (11) and the second gauge hole (12) are both cylindrical holes, the diameter of the first gauge hole (11) is 8-15mm, and the diameter of the second gauge hole (12) is 3-6mm.
  7. 7. The deep-well plate according to claim 6, characterized in that the depth of the first gauge hole (11) is 15-30mm.
  8. 8. Deep-well plate according to claim 1, characterized in that the thickness of the partition wall between adjacent first gauge holes (11) and second gauge holes (12) is 2-4mm.
  9. 9. Deep hole plate according to any of claims 1-8, characterized in that the deep hole plate (1) is made of polypropylene material and vertical ribs are provided on the inner walls of the first gauge holes (11) and the second gauge holes (12).
  10. 10. Deep-well plate according to any of claims 1-8, further comprising a sealing membrane, which sealing membrane detachably seals the first gauge hole (11) and the second gauge hole (12) of the deep-well plate (1).

Description

Deep hole plate suitable for high flux extraction of DNA of large-volume seed Technical Field The utility model relates to the technical field of detection equipment, in particular to a deep hole plate suitable for high-throughput DNA extraction of large-volume seeds. Background With the development of modern molecular breeding and genetic research, the extraction and analysis of large-scale plant genomic DNA has become an important requirement in the biotechnology field. In a plurality of plant samples, DNA extraction of large-volume seeds such as corn has specificity, which is not only reflected in physical characteristics such as large volume of seeds and hard tissue, but also reflected in special requirements on equipment and consumables during batch processing. At present, DNA extraction in a laboratory generally adopts consumable materials with standard specifications such as 96-well plates, and the design is mainly aimed at small-volume samples such as blades, and has obvious defects when large-volume seeds are treated. The aperture of standard 96-well plates is typically below 7mm, which is too small for large volumes of seeds with diameters of 8-12mm, such as corn, resulting in the inability to fit smoothly into the seeds or subsequent processing after placement due to limited space. Although the accommodation problem can be solved by increasing the aperture, simply enlarging all the apertures causes new problems that firstly, the partition wall between adjacent apertures is thinned, the strength of the plate is reduced, cracking is easy to generate in the vibration grinding process, secondly, the aperture of the first specification can cause the increase of the reagent amount in the pipetting operation, reagent waste is caused, and thirdly, the whole size of the deep aperture plate exceeds the standard specification by adopting the aperture of the first specification, so that the deep aperture plate cannot be compatible with the existing automatic equipment. There are laboratories attempting to process large volumes of seeds by changing to large volumes of centrifuge tubes or test tubes, but this solution is difficult to automate, and manual processing is not only inefficient but also prone to operating errors. It has also been proposed to use a specially made first gauge aperture deep well plate, but because of the inability to work with standard 96 channel pipetting stations, experimental efficiency and operational convenience are severely compromised. Disclosure of utility model The utility model provides a deep hole plate suitable for high-flux DNA extraction of large-volume seeds, which can meet the requirement of large-volume seed treatment and maintain the compatibility with the existing automatic equipment. The embodiment of the utility model provides a deep hole plate suitable for high-throughput DNA extraction of large-volume seeds, wherein a plurality of first specification holes and a plurality of second specification holes are formed in the deep hole plate, the first specification holes and the second specification holes are alternately arranged on the deep hole plate at intervals, the diameter of the first specification holes is larger than that of the second specification holes, and the first specification holes are used for accommodating the large-volume seeds. In one possible implementation, 48 first gauge holes and 48 second gauge holes are provided in the deep well plate. In one possible implementation, the center-to-center spacing of adjacent first gauge holes and second gauge holes matches the gun head spacing of a 96-channel high-throughput workstation. In one possible implementation, the first gauge holes and the second gauge holes are arranged in 8 rows and 12 columns or 12 rows and 8 columns. In one possible implementation, the deep hole plate includes a first deep hole plate and a second deep hole plate having the same structure, wherein a first specification hole on the first deep hole plate corresponds to a second specification hole on the second deep hole plate, and a second specification hole on the first deep hole plate corresponds to a first specification hole on the second deep hole plate. In one possible implementation, the first gauge hole and the second gauge hole are both cylindrical holes, the first gauge hole having a diameter of 8-15mm and the second gauge hole having a diameter of 3-6mm. In one possible implementation, the first gauge hole has a depth of 15-30mm. In one possible implementation, the thickness of the partition wall between adjacent first gauge holes and second gauge holes is 2-4mm. In one possible implementation manner, the deep-hole plate is made of polypropylene, and vertical reinforcing ribs are arranged on the inner walls of the hole walls of the first-specification holes and the second-specification holes. In one possible implementation, the deep well plate further includes a sealing membrane that removably seals the first gauge hole and