CN-224227018-U - Conductive clamping groove

CN224227018UCN 224227018 UCN224227018 UCN 224227018UCN-224227018-U

Abstract

The utility model relates to a conductive clamping groove which is applied to detection of the same-wafer space multiunit, wherein the space multiunit comprises a space transcriptome and a space metabolome, the conductive clamping groove comprises a conductive bar, a plurality of grooves are arranged on the conductive bar at equal intervals, the size of each groove is matched with the size of a target space transcriptome chip, wafer taking holes are arranged at four corners of each groove, the wafer taking holes are used for taking out the target space transcriptome chip from the grooves, and the depth of each groove is matched with the height of the target space transcriptome chip within a specified error range. Therefore, the carrier is provided for realizing the synchronous detection of the high-resolution space transcriptome and the metabolome of the same tissue slice, the difficult problem of the conductivity conflict of two histology is solved in a breakthrough way, and the key technical support is provided for the space integration research of multiple histology.

Inventors

HUI LIJIAN
WANG GANGQI
WU BAIHUA

Assignees

中国科学院分子细胞科学卓越创新中心
复旦大学

Dates

Publication Date: 20260512
Application Date: 20250527

Claims (7)

1. The conductive clamping groove is applied to detection of the same-chip space multiple-study, wherein the space multiple-study comprises a space transcriptome and a space metabolome, and is characterized by comprising a conductive row; arranging a plurality of grooves on the conducting bar at equal intervals, wherein the size of each groove is matched with the size of the target space transcriptome chip; A chip taking hole is arranged at four corners of each groove, wherein the chip taking hole is used for taking out the target space transcriptome chip from the groove; the depth of each groove matches the height of the target spatial transcriptome chip within a specified error range.
2. The conductive card slot of claim 1, wherein the specified error range is 0-10 μm.
3. The conductive card slot of claim 1, wherein the conductive strip is made of a conductive metal material, and the conductive metal material comprises brass.
4. The conductive card slot of claim 1, wherein a boss is provided below the conductive strip.
5. The conductive card slot of claim 4, wherein the land length is greater than the length between the side edge grooves and less than the total length of the conductive row.
6. The conductive card slot of claim 1, wherein a designated distance is provided between the outer edges of the two sides in the length direction of the conductive bar and the edge groove.
7. The conductive card slot of claim 1, wherein the target space transcriptome chip comprises a Stereo-seq chip.

Description

Conductive clamping groove Technical Field The utility model mainly relates to the field of space histology, in particular to a conductive clamping groove. Background In recent years, the rapid development of spatial transcriptome and spatial metabolome technologies has enabled the in situ horizontal resolution of biological processes in tissues, providing an unprecedented tool for studying spatial heterogeneity in complex tissues. In the field of spatial transcriptome, techniques such as spatial gene expression (Visium) and spatial transcriptome (SeqScope) have been widely used in disease research, for example, to resolve spatial features of liver lobules in primary and metastatic liver cancer. However, these techniques still have significant limitations in that Visium resolution is only 50 μm, which makes it difficult to achieve spatial resolution at the single cell level, while SeqScope, while achieving ultra-high resolution of approximately 1 μm, has a detection area of only 0.2mm 2, which limits large-scale tissue analysis. In contrast, the space-time histology (Stereo-seq) technology based on DNA nanospheres has ultrahigh resolution, ultra-large field of view and high capture efficiency, so that the technology has obvious advantages in research of mouse organ development, tumor microenvironment, primate cerebral cortex and the like. At the same time, spatial metabonomics enables in situ detection of metabolites by mass spectrometry imaging techniques (e.g., MALDI-Orbitrap, DESI and SIMS), enabling the revealing of metabolic heterogeneity on the micrometer or even single cell scale (5 μm). These techniques have been used to study key biological problems such as liver metabolic compartmentalization, host-microorganism interaction, cell fate decisions, and metabolic microenvironment effects. For example, in mouse and human liver studies, MALDI-Orbitrap and DESI techniques reveal metabolic spatial features (50 μm resolution) of steady state and fatty liver, whereas in combination with isotopically labeled spatial metabolic flow analysis techniques scientists have been able to track metabolic dynamics at the single cell level and map with high accuracy in human kidney development and injury studies. The complementary combination of the techniques provides a brand new view for decoding cell interaction and metabolic heterogeneity at a tissue level, not only can dynamically change genes and metabolic molecules in a life process be analyzed from multiple dimensions, but also key signal paths and metabolic products for driving spatial heterogeneity can be identified based on a large-scale high-throughput data mining interaction regulation network of genes and metabolic products, so that a complex biological mechanism is systematically explained. Although spatial transcriptome and spatial metabolome techniques are increasingly used in the field of spatial biology, existing joint analysis schemes still have significant limitations in that most studies either perform the joint analysis by serial sections alone or separately detect at lower resolution (100 μm) on the same tissue section. Due to technical limitations, the strategy of adjacent sections or low resolution is only suitable for macroscopic region research, accurate analysis of microenvironment and even single cell level is difficult to realize, and deep exploration of complex biological problems is greatly restricted. The core challenge of realizing the same-chip high-resolution detection is the principle difference of two technologies, namely, the space transcriptome technologies such as Stereo-seq and the like rely on a DNA Nanosphere (DNB) chip to capture RNA and carry out high-throughput sequencing, and the space metabolome technologies such as matrix assisted laser desorption ionization mass spectrometry (MALDI-MSI) and the like need to directly acquire mass-to-charge ratio space distribution data by exciting metabolic molecule ionization in a tissue slice through laser. The technical suitability of the two is basically conflicted that a non-conductive bar code chip is needed to be used for a space transcriptome, and MALDI-MSI is needed to be based on a conductive ITO glass slide, and in addition, RNA degradation can be caused by laser, matrix spraying and other operations in the mass spectrum imaging process, so that the application of co-chip integration is further hindered. Disclosure of utility model An object of the present utility model is to provide a conductive clamping groove, which solves the problem that two kinds of space group science synchronous detection cannot be performed on the same tissue slice in the prior art. According to one aspect of the utility model, there is provided a conductive card slot for use in co-chip space multi-study detection, the space multi-study including a space transcriptome and a space metabolome, the conductive card slot including a conductive strip; arranging a plurality of grooves on the conducting bar