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CN-224227052-U - Sample pretreatment pipe

CN224227052UCN 224227052 UCN224227052 UCN 224227052UCN-224227052-U

Abstract

A sample pretreatment tube relates to a biomedical research and development and detection device, which is used for pretreatment of liquid biological samples. The sample pretreatment tube consists of an embedded upper tube and a lower tube, wherein a tube cap is covered above the upper tube, an upper groove and a lower groove are formed in the outer wall surface of the upper tube, the lower tube is sleeved outside the upper tube, the inner wall of the lower tube is tightly adhered to the outer wall of the upper tube and can slide relatively, a buckle is arranged at the upper edge of the lower tube, the buckle can be embedded in the upper groove or the lower groove to determine the relative position of the upper tube and the lower tube, and an upper filter element and a lower filter element are arranged in the upper tube. The pretreatment device has the beneficial effects that pretreatment of 2 steps can be completed in one sample tube, so that the steps of sample pretreatment are greatly simplified, and the consumption of manpower and time is reduced.

Inventors

  • XU JIN
  • QIAN WEIZHU
  • HOU SHENG
  • GUO QINGCHENG
  • LI JIYANG
  • YANG LIMIN

Assignees

  • 泰州迈博太科药业有限公司

Dates

Publication Date
20260512
Application Date
20250414

Claims (6)

  1. 1. A sample pretreatment tube is characterized by comprising an upper tube (1) and a lower tube (2) which are embedded, wherein a tube cap (9) is covered above the upper tube (1), an upper groove (11) and a lower groove (12) are formed in the outer wall surface of the upper tube (1), the lower tube (2) is sleeved outside the upper tube (1), the inner wall of the lower tube (2) is tightly adhered to the outer wall of the upper tube (1) and can slide relatively, a buckle (21) is arranged at the upper edge of the lower tube (2), the buckle (21) can be embedded in the upper groove (11) or the lower groove (12) to determine the relative positions of the upper tube (1) and the lower tube (2), and an upper filter element (3) and a lower filter element (4) are arranged in the upper tube (1).
  2. 2. Sample pretreatment tube according to claim 1, characterized in that the pore size of the upper filter element (3) is between 2 and 10 microns.
  3. 3. The sample pretreatment tube according to claim 1, wherein a solid reagent a (5) containing a pretreatment reagent is provided above the upper filter element (3).
  4. 4. The sample pretreatment tube according to claim 1, wherein a solid reagent B (6) containing a pretreatment reagent is provided above the lower filter element (4).
  5. 5. A sample pretreatment tube according to claim 3, wherein the solid pharmaceutical agent a (5) has a sheet shape.
  6. 6. The sample pretreatment tube according to claim 4, wherein the solid drug B (6) has a shape of a tablet or a pellet.

Description

Sample pretreatment pipe Technical Field The utility model relates to a biomedical research and development and detection device, in particular to a sample pretreatment tube. Background Biological medicine development and medical testing often require the detection of biological samples, including blood, tissue fluids, cell wall-broken extracts, and the like, including electrophoresis, enzyme-linked immunosorbent assay (ELISA), liquid chromatography, mass spectrometry, and the like. The pretreatment of the sample is usually required before detection, impurities affecting detection are removed as much as possible, and the protein or nucleic acid to be detected in the sample is subjected to enzyme digestion, reduction, denaturation, hydrolysis and the like. In large-scale detection, pretreatment of the sample requires a lot of labor and time. If the pretreatment of the sample can be simplified, the detection and inspection efficiency can be greatly improved. Disclosure of Invention The utility model provides a sample pretreatment tube which is used for pretreatment of liquid biological samples. The technical scheme of the utility model is as follows: a sample pretreatment tube (fig. 1, 2) comprises an upper tube 1 and a lower tube 2 which are embedded, wherein a tube cap 9 is covered above the upper tube 1. The outer wall surface of the upper pipe 1 is provided with 2 grooves, namely an upper groove 11 and a lower groove 12. The lower pipe 2 is sleeved outside the upper pipe 1, and the inner wall of the lower pipe 2 is closely adhered to the outer wall of the upper pipe 1 and can slide relatively. The upper edge of the lower tube 2 is provided with a buckle 21, and the buckle 21 can be embedded in the upper groove 11 or the lower groove 12 to determine the relative position of the upper tube 1 and the lower tube 2. The longitudinal sectional view of the sample pretreatment tube when the snap 21 is fitted in the upper groove 11 is shown in FIG. 1. The longitudinal sectional view of the sample pretreatment tube when the snap 21 is fitted in the lower groove 12 is shown in FIG. 2. The upper pipe 1 is internally provided with 2 filter elements, namely an upper filter element 3 and a lower filter element 4. Preferably, the pore diameter of the upper filter element 3 is 2 to 10 micrometers, and the pore diameter of the lower filter element 4 is determined according to the permeation pore diameter of the molecules to be detected. Optionally, a solid medicament A-5 containing a pretreatment reagent is arranged above the upper filter element 3. Optionally, a solid medicament B-6 containing a pretreatment reagent is arranged above the lower filter element 4. The solid medicament A-5 is in a sheet shape, and the dosage form is a dispersible tablet. The solid medicament B-6 is in a sheet shape or a particle shape, and the dosage form is a dispersible tablet or a dispersible particle. The dispersible tablet or the dispersible granule is added with disintegrating agents such as sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, crospovidone or croscarmellose sodium, and can be dissolved rapidly when meeting water. The dispersible tablet is added with a surfactant such as sodium dodecyl sulfate. The application method of the utility model is as follows: In the first step, before the sample pretreatment tube is used, the clamp 21 is clamped at the position of the upper groove 11, the tube cap 9 is opened, the liquid sample is added into the upper tube 1, and the state of the sample pretreatment tube and the sample 30 after the addition is finished is shown in fig. 3A. The tube cap 9 is covered, the tube body is gently shaken to promote mixing of the liquid sample and the solid pharmaceutical agent A, and then the sample pretreatment tube is left for a period of time to complete the reaction. And secondly, opening the tube cap 9, moving the lower tube 2 to clamp the clamp 21 at the position of the lower groove 12, and enabling the sample 30 to pass through the upper filter element 3 to be contacted and mixed with the solid medicament B under the action of negative pressure, wherein the state of the sample pretreatment tube and the sample 30 is shown in fig. 3B. The cap 9 is covered, the tube body is gently shaken to promote mixing of the liquid sample 30 and the solid drug B, and then the sample pretreatment tube is left for a period of time to complete the reaction. And thirdly, placing the sample pretreatment tube into a centrifuge for centrifugation, allowing the liquid sample 30 to fall into the bottom of the lower tube 2 through the lower filter element 4 under the action of centrifugal force, and removing the upper tube 1 to take out the pretreated sample 30 in the lower tube 2. The pretreatment device has the beneficial effects that pretreatment of 2 steps can be completed in one sample tube, so that the steps of sample pretreatment are greatly simplified, and the consumption of manpower and time is