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CN-224227053-U - Microorganism vacuum preservation tube

CN224227053UCN 224227053 UCN224227053 UCN 224227053UCN-224227053-U

Abstract

The utility model discloses a microorganism vacuum preservation tube which comprises a tube body and two cover bodies matched with each other, wherein the tube body is sequentially provided with a storage area, a fusing area and an opening area from bottom to top, the cover body A is fixedly connected with the opening area and is used for sealing the tube body, an axial through hole is axially formed in the cover body A, a filter plug is arranged in the axial through hole, one end of the filter plug is fixed in the axial through hole, the other end of the filter plug extends into the opening area, and the cover body B is detachably arranged on the cover body and is used for sealing the axial through hole. The utility model eliminates the potential pollution risks of the strain in the freeze-drying, vacuum and fusing processes in the tube body 1, ensures the stability of the strain in long-term storage, and has the characteristics of simple structure, low cost, convenience and practicability.

Inventors

  • ZHANG CHAO
  • XIE RONGXIAN
  • Li Ruoyue
  • HUANG QIAN
  • FU MING
  • ZHANG NIAN
  • WANG XINYUE
  • HUANG FANG

Assignees

  • 复星安特金(成都)生物制药股份有限公司

Dates

Publication Date
20260512
Application Date
20250516

Claims (13)

  1. 1. A microorganism vacuum preservation tube is characterized by comprising a tube body (1) and two cover bodies which are matched with each other for use, wherein the tube body (1) is sequentially provided with a storage area (4), a fusing area (5) and an opening area (6) from bottom to top, the cover body A (2) is detachably connected with the opening area (6) and is used for sealing the tube body (1), an axial through hole (7) is formed in the cover body A (2), a filter plug (8) is arranged in the axial through hole (7), one end of the filter plug (8) is arranged in the axial through hole (7), the other end of the filter plug extends into the opening area (6), and the cover body B (3) is detachably arranged on the cover body A (2) and is used for sealing the axial through hole (7).
  2. 2. The microorganism vacuum preservation tube according to claim 1, wherein the outer wall of the cover body a (2) is provided with a protrusion (9), and the inner wall of the cover body B (3) is provided with a protruding point (10) matched with the protrusion (9) for being buckled with the cover body a (2).
  3. 3. A tube for the vacuum preservation of microorganisms according to claim 2, characterized in that said projections (9) are of annular configuration, and said plurality of projections (10) are provided and are uniformly distributed in an annular configuration.
  4. 4. The microorganism vacuum preservation tube according to claim 1, wherein the outer wall of the cover body a (2) is provided with a groove (11), and the groove (11) is positioned at the buckling position of the cover body a (2) and the cover body B (3) and is used for scratching the cover body B (3).
  5. 5. A tube for preserving microorganisms in vacuum according to claim 1, wherein the top of the filter plug (8) is aligned with the top of the axial through hole (7), and the upper end of the side wall of the filter plug (8) is fixedly connected or integrally formed with the upper end of the inner wall of the axial through hole (7).
  6. 6. The microorganism vacuum preservation tube according to claim 5, wherein the area of the connection part of the filter plug (8) and the inner wall of the axial through hole (7) is 1/3-2/3 of the area of the inner wall of the axial through hole (7).
  7. 7. The microorganism vacuum preservation tube according to claim 1, wherein the axial through hole (7) is in a cylindrical structure, an annular flange (12) is arranged on the inner side of the top of the axial through hole (7), the annular width of the flange (12) is 1/10-1/3 of the diameter of the cover body A (2), and the top of the filter plug (8) is fixedly connected with the inner wall of the flange (12) or integrally formed.
  8. 8. A tube for the vacuum preservation of microorganisms according to claim 7, characterized in that the upper end of the side wall of the filter plug (8) is fixedly connected or integrally formed with the upper end of the inner wall of the axial through hole (7).
  9. 9. The microorganism vacuum preservation tube of claim 8, wherein the area of the connection part of the filter plug (8) and the inner wall of the axial through hole (7) is 1/3-2/3 of the area of the inner wall of the axial through hole (7).
  10. 10. A tube for the vacuum preservation of microorganisms according to any one of claims 1 to 9, characterized in that the cover a (2) is screwed to the opening area (6).
  11. 11. A tube for the preservation of microorganisms according to any one of claims 1 to 9, characterized in that the plug (8), the cover a (2) and/or the cover B (3) are made of plastic, rubber or silica gel.
  12. 12. A tube for the vacuum preservation of microorganisms according to any one of claims 1 to 9, characterized in that the thickness of the cover a (2) is 1 to 2cm, the thickness of the cover B (3) is 0.5 to 1cm, and the length of the plug (8) is 2 to 3cm.
  13. 13. A tube for the vacuum preservation of microorganisms according to any one of claims 1 to 9, wherein gaskets or seals are provided for sealing within the cover a (2) and the cover B (3).

Description

Microorganism vacuum preservation tube Technical Field The utility model provides a microorganism vacuum preservation tube, in particular relates to a device for microorganism strain preservation, and belongs to the technical field of strain preservation. Background Microbial strains are increasingly frequently used in scientific research experiments, and effective preservation of the microbial strains is important to guaranteeing activity and stability of the microbial strains. Improper preservation is liable to cause strain pollution, mutation or death, so that a reliable method is required to enable the strain to stay in a dormant survival state for a long time. The conventional preservation methods at present comprise a liquid paraffin method, a glycerol method, a magnetic bead method, an inclined plane method, a liquid nitrogen method and a vacuum freeze-drying method, wherein the vacuum freeze-drying method has remarkable comprehensive advantages in the aspects of preservation aging, strain stability and storage convenience. The existing strain vacuum preservation tube mostly adopts a straight tube structure and is divided into an opening area, a fusing area and a strain storage area. In general, after a certain volume of liquid spawn is filled into a storage area at the bottom of the tube body, the opening area is plugged by cotton or other filter plugs, so that pollution is prevented and a certain degree of ventilation is ensured. And then placing the strain preservation tube in a plugged state in a freeze dryer for strain freeze-drying, and discharging water through a filter plug of an opening area in the freeze-drying process. After the freeze-drying procedure is finished, the strain preservation tube is taken out, a filter plug part outside the tube body is cut off by scissors, then the opening area of the strain tube is connected with a vacuum pump interface, air in the tube is pumped by a vacuum pump to keep vacuum, finally, the glass tube body is melted in a fusing area by spraying flame and sealed at the same time, and finally, the stored strain is in a freeze-dried and vacuum state in the tube and is preserved at a low temperature, so that the long-term stability of the stored strain is ensured. In the actual operation process, the existing vacuum strain preservation tube has at least the following defects: (1) When using scissors to trim the excess plug, it is easy to cause the plug to loosen, resulting in a potential contamination risk. (2) When the freeze drying of the strain is finished, after the preserving pipe is taken out from the freeze dryer, a certain time is required to start the vacuumizing operation, and at this interval, because air circulation exists in the filter plug, moisture in the air is easy to enter a strain storage area, so that the strain drying state is damaged, the moisture content of a sample is increased, and the long-term storage stability of the strain is affected. In view of the foregoing, there is a need to design a new strain freeze-drying vacuum preservation tube to solve the above-mentioned drawbacks. In the prior art, as disclosed in the patent application of the utility model with the publication number of CN115369015A, the improvement of the outer cover of the preservation tube is mainly used for reducing the air inlet amount when extracting the strains, while the bottle cap for the microorganism strain preservation device disclosed in the patent application with the publication number of CN207417492U mainly solves the connection mode of the bottle cap and the tube body. Therefore, the prior art does not provide a solution to the above or related problems. Disclosure of utility model The utility model aims to solve the problem that the strain is in a closed state all the time due to the fact that the strain enters the strain storage area to damage the strain drying state due to the fact that air circulation exists in the filter plug during the freeze-drying and vacuumizing interval, and meanwhile, the problem that the filter plug is loosened due to the fact that the redundant filter plug needs to be cut off before vacuumizing is solved, and potential pollution risks are avoided. The utility model is realized by the following technical scheme: a microorganism vacuum preservation tube comprises a tube body and two cover bodies matched with each other, wherein the tube body is sequentially provided with a storage area, a fusing area and an opening area from bottom to top, the cover body A is detachably connected with the opening area and used for sealing the tube body, an axial through hole is formed in the cover body A, a filter plug is arranged in the axial through hole, one end of the filter plug is arranged in the axial through hole, the other end of the filter plug extends into the opening area, and the cover body B is detachably arranged on the cover body A and used for sealing the axial through hole. Preferably, the cover body B is detachably arrang