CN-224227072-U - Nucleic acid amplification reaction module and nucleic acid amplification instrument

Abstract

The application discloses a nucleic acid amplification reaction module and a nucleic acid amplification instrument. The nucleic acid amplification reaction module comprises a reactor, a semiconductor refrigeration piece and a radiator, wherein the semiconductor refrigeration piece is contacted with the outer side wall of the reactor to heat/cool the reactor. Compared with the prior art, the application changes the heating/cooling mode of heat transfer at the bottom of the reactor into heat transfer at the side wall of the reactor, thereby increasing the heat transfer area, shortening and changing the heat transfer path and greatly improving the heating efficiency and the heating uniformity.

Inventors

• QIN RONG

Assignees

• 上海宏石医疗科技有限公司

Dates

Publication Date

20260512

Application Date

20250122

Claims (9)

1. 1. The nucleic acid amplification reaction module is characterized by comprising a reactor, a semiconductor refrigeration piece and a radiator, wherein the semiconductor refrigeration piece is thermally connected with the outer side wall of the reactor and is thermally connected with the radiator, annular protrusions are arranged along the outer side wall of the reactor, the semiconductor refrigeration piece is annular, the lower part of the reactor passes through the semiconductor refrigeration piece, and the semiconductor refrigeration piece is attached to the annular protrusions and the radiator.
2. 2. The nucleic acid amplification reaction module of claim 1, wherein the annular projection extends from a position in the middle of the outer side wall of the reactor to a position in the middle of the outer side wall of the reactor in the vertical direction.
3. 3. The nucleic acid amplification reaction module of claim 2, wherein the annular protrusion comprises a vertical section and an inclined section from bottom to top, the vertical section is provided with an optical fiber hole, and the surface of the inclined section is inclined from bottom to top toward the central axis of the reactor.
4. 4. The nucleic acid amplification reaction module of claim 1, wherein the heat sink is provided with a groove, and the lower portion of the reactor passes through the semiconductor cooling fin and is accommodated in the groove without thermal connection with the heat sink.
5. 5. The nucleic acid amplification reaction module of claim 4, wherein the reactor is fixedly connected to the semiconductor cooling fin, and the semiconductor cooling fin is fixedly connected to the heat sink.
6. 6. The nucleic acid amplification reaction module according to claim 4, wherein the reactor is fixedly connected to the heat sink via a connector, the semiconductor refrigeration sheet is sandwiched between the reactor and the heat sink, the connector is a heat insulating material, or a heat insulating material is provided between the reactor and the connector, and a heat insulating material is provided between the heat sink and the connector.
7. 7. The nucleic acid amplification reaction module of any one of claims 1 to 6, wherein the reactor, the semiconductor cooling fin, and the heat sink are one.
8. 8. A nucleic acid amplification apparatus comprising the nucleic acid amplification reaction module of any one of claims 1 to 7.
9. 9. The nucleic acid amplification apparatus of claim 8, wherein the nucleic acid amplification reaction modules are arranged in a geometric array, and the temperature of the reactor in each nucleic acid amplification reaction module is independently controllable.

Description

Nucleic acid amplification reaction module and nucleic acid amplification instrument Technical Field The application belongs to the technical field of nucleic acid amplification instruments, and particularly relates to a nucleic acid amplification reaction module and a nucleic acid amplification instrument. Background The nucleic acid amplification instrument is a basic instrument for PCR reaction in the biomedical field, the rapid heating/cooling response is needed to be realized in the PCR reaction, and the uniform and stable temperature environment is needed to be maintained in the reaction process. The PCR reaction is carried out in a test tube in the reactor, the outer wall of the test tube is attached to the inner wall of the reactor, the reactor is heated by the semiconductor refrigerating sheet for providing heat for the reactor, and the heat of the reactor is firstly transferred to the semiconductor refrigerating sheet during heat dissipation and then dissipated through a radiator. In the prior art, a semiconductor refrigerating sheet is usually contacted with the bottom of a reactor to heat/cool the reactor, and the heating/cooling mode has two defects, namely, the contact area is smaller, the heat transfer efficiency is lower, more importantly, the heating/cooling mode can lead to heat transfer between the bottom and the top of the reactor, and the heat transfer path is longer, so that the bottom and the top of the reactor have temperature difference, which is unfavorable for PCR reaction. In view of the above, there is a need for a nucleic acid amplification reaction module and a nucleic acid amplification apparatus that have a simple structure and are capable of improving heating/cooling efficiency and heating/cooling uniformity. Disclosure of utility model In view of the above-mentioned drawbacks or shortcomings of the prior art, the present application is directed to a nucleic acid amplification reaction module and a nucleic acid amplification apparatus. In order to solve the technical problems, the application is realized by the following technical scheme: The application provides a nucleic acid amplification reaction module which is characterized by comprising a reactor, a semiconductor refrigerating sheet and a radiator, wherein the semiconductor refrigerating sheet is thermally connected with the outer side wall of the reactor and is thermally connected with the radiator. Optionally, the nucleic acid amplification reaction module is characterized in that an annular protrusion is disposed along an outer side wall of the reactor, the semiconductor cooling sheet is annular, the lower portion of the reactor passes through the semiconductor cooling sheet, and the semiconductor cooling sheet is attached to the annular protrusion and the radiator. Alternatively, in the nucleic acid amplification reaction module described above, wherein the annular projection extends from a position in the middle of the outer side wall of the reactor to a position in the middle of the outer side wall of the reactor in the vertical direction. Optionally, in the nucleic acid amplification reaction module, the annular protrusion includes a vertical section and an inclined section from bottom to top, an optical fiber hole is formed in the vertical section, and a surface of the inclined section is inclined from bottom to top toward a central axis direction of the reactor. Optionally, in the nucleic acid amplification reaction module, a groove is formed in the heat sink, and the lower portion of the reactor passes through the semiconductor refrigeration sheet and is accommodated in the groove, so that thermal connection with the heat sink is not generated. Optionally, in the nucleic acid amplification reaction module, the reactor is fixedly connected with the semiconductor refrigeration piece, and the semiconductor refrigeration piece is fixedly connected with the radiator. Optionally, in the nucleic acid amplification reaction module, the reactor may be fixedly connected with the radiator through a connecting piece, the semiconductor refrigeration piece is clamped between the reactor and the radiator, the connecting piece is made of a heat insulation material, or a heat insulation material is arranged between the reactor and the connecting piece, and a heat insulation material is arranged between the radiator and the connecting piece. Optionally, the nucleic acid amplification reaction module described above, wherein the reactor, the semiconductor cooling fin, and the heat sink are all one. The application also provides a nucleic acid amplification instrument which is characterized by comprising the nucleic acid amplification reaction module. Optionally, the nucleic acid amplification apparatus as described above, wherein the nucleic acid amplification reaction modules are arranged in a geometric array, and the temperature of the reactor in each nucleic acid amplification reaction module may be controlled individually