CN-224227085-U - High-flux cell screening plate

Abstract

A high-throughput cell screening plate belongs to a biomedical research and development device and is used for qualitatively or quantitatively screening cells expressing or secreting specific proteins or other biochemical molecules from a group of cells. The device comprises a bottom plate, a grid and a cover plate, wherein a large groove with a flat bottom is arranged at the central part of the bottom plate, small grooves are arranged at the edges of the bottom plate, and the grid is of a grid-shaped structure and can be embedded in the large groove to divide the space in the groove. The utility model has the advantages that the screening flux and the consumed time are superior to those of a limiting dilution method, the manufacturing cost and the difficulty of operation are superior to those of a micro engraving method, and the utility model is applicable to the conditions that the screening marker is expressed on the surface of a cell and secreted in the environment.

Inventors

• XU JIN
• QIAN WEIZHU
• HOU SHENG
• GUO QINGCHENG
• LI JIYANG
• YANG LIMIN

Assignees

• 泰州迈博太科药业有限公司

Dates

Publication Date

20260512

Application Date

20250414

Claims (5)

1. 1. The utility model provides a high flux cell screening board, its characterized in that includes bottom plate (1), net bars (2), apron (3), bottom plate (1) look down and be rectangle, the central part has a big recess (4) that the bottom is leveled, there is little recess (5) on the edge, net bars (2) be the grid-like structure, can gomphosis in big recess (4) and cut apart the recess inner space, apron (3) can lock on bottom plate (1), play airtight and guard action.
2. 2. The high throughput cell screening plate according to claim 1, wherein the bottom plate (1) is provided with a level meter (6) for detecting the level condition of the bottom plate (1), and four corners of the bottom plate (1) are provided with adjusting knobs (7) for adjusting the level of the bottom plate (1).
3. 3. The high throughput cell screening plate according to claim 1, wherein the cover plate (3) is provided with an antibacterial ventilation hole (8).
4. 4. The high throughput cell screening plate according to claim 1, wherein the substrate (1) is made of polystyrene, polypropylene, polycarbonate or polymethyl methacrylate.
5. 5. The high throughput cell screening plate according to claim 1, wherein the mesh (2) is made of polystyrene, polypropylene, polycarbonate, polymethyl methacrylate, stainless steel or titanium alloy.

Description

High-flux cell screening plate Technical Field The utility model relates to a biomedical research and development device, in particular to a high-flux cell screening plate. Background The biological macromolecular medicine is one kind of biological macromolecules produced with modern biotechnology and used in disease diagnosis, treatment and prevention, including monoclonal antibody, recombinant protein, polypeptide, enzyme, cell factor, etc. The medicine has the characteristics of strong targeting, high specificity, low toxic and side effects, high sensitivity and the like, and has obvious curative effects on the treatment of malignant tumors, immune system diseases, metabolic diseases, genetic diseases, infectious diseases and other serious diseases. Cell screening refers to screening out cells with characteristics meeting requirements from a group of cells, and monoclonal and amplification, and is an important technology used for antibody screening (such as hybridoma screening, single B cell screening and the like) and engineering cell strain screening for producing antibodies. Existing cell screening methods include limiting dilution methods, flow cytometry, micro-engraving techniques (microengraving), and the like. The method has the respective limitations that the limiting dilution method has the problems of heavy labor work and long screening time, the flow cytometry is only suitable for the condition that cell screening markers are expressed on the cell surface, and the micro engraving technology requires photoetching equipment and has high operation requirements on technicians. Disclosure of Invention The present utility model provides a high throughput cell screening plate for qualitatively or quantitatively screening cells expressing or secreting a particular protein or other biochemical molecule from a population of cells. The technical scheme of the utility model is as follows: A high-throughput cell screening plate, as shown in figures 1 and 2, comprises a bottom plate 1, a grid 2 and a cover plate 3. The bottom plate 1 is rectangular in top view, a large groove 4 with a flat bottom is arranged in the central part, a small groove 5 is arranged on the edge of the large groove 4, a semisolid culture medium containing cells is paved in the large groove 4, and the small groove 5 is used for containing water or aqueous solution to adjust the humidity of the small environment. Optionally, the bottom plate 1 is provided with a level gauge 6 for detecting the level condition of the bottom plate 1, and four corners of the bottom plate 1 are provided with adjusting knobs 7 for adjusting the level of the bottom plate 1. The grid 2 is a grid structure, can be embedded in the large groove 4 and divides the space in the groove into a plurality of small blocks. The cover plate 3 is buckled on the bottom plate 1 to play a role in sealing and protecting. Optionally, the cover plate 3 is provided with an antibacterial ventilation hole 8. The bottom plate 1 is made of transparent hard plastic such as polystyrene, polypropylene, polycarbonate or polymethyl methacrylate, and the grid 2 is made of transparent hard plastic or metal with stable property and corrosion resistance such as stainless steel and titanium alloy. The application method of the utility model is as follows: The first step is to place the base plate 1 on an experimental platform, and to determine that the base plate 1 is in a horizontal state with the aid of the level gauge 6 through the adjusting knob 7. And the second step, pouring the semisolid culture solution containing the cells into the large groove 4 to be flatly laid on the bottom surface, placing the grid 2 into the large groove 4, injecting water into the small groove 5, covering the cover plate 3, and placing into a cell incubator for culturing for a period of time. Thirdly, adding antibodies or antigens marked by fluorescein or pigment (the antibodies or antigens which have no influence on the activity of cells and have stable properties can also be added into a cell culture solution before culturing) into the large groove 4, and putting into a detector to qualitatively and quantitatively detect cells marked positive. And step four, taking out the positive cell colony from the semisolid culture medium through microscopic operation, and carrying out monoclonalization, amplification and further identification, thereby obtaining the cell strain with the characteristics meeting the requirements. The utility model has the advantages that the screening flux and the consumed time are superior to those of a limiting dilution method, the manufacturing cost and the difficulty of operation are superior to those of a micro engraving method, and the utility model is applicable to the conditions that the screening marker is expressed on the surface of a cell and secreted in the environment. Drawings FIG. 1 is a longitudinal cross-sectional view of a high throughput cell screening plate prior to as