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CN-224227086-U - Stem cell exosome bioreactor for culture

CN224227086UCN 224227086 UCN224227086 UCN 224227086UCN-224227086-U

Abstract

The utility model relates to the technical field of exosome preparation, in particular to a bioreactor for stem cell exosome culture, which comprises a workbench, wherein the front surface of the workbench is fixedly connected with a control panel, the top surface of the workbench is provided with a rotating disc, the lower end of the rotating disc is provided with a driving motor, the driving motor is arranged in the workbench, the top surface of the rotating disc is sequentially and fixedly connected with a mounting column and a positioning rod from left to right, and the top surfaces of the mounting column and the positioning rod are provided with mounting seats. According to the utility model, the mounting seat and the culture cylinder can be driven to stably rotate through the rotating disc, then the mounting motor is started, and the rotating shaft and the stirring assembly are driven to rotate through the mounting motor, so that the culture solution and cells in the culture cylinder can be uniformly rotated through the design of the rotating shaft and the stirring assembly, and the culture solution and the cells can be uniformly mixed.

Inventors

  • WU JINYI
  • Shao Xiechun

Assignees

  • 浙江思丹姆干细胞生物科技有限公司

Dates

Publication Date
20260512
Application Date
20250416

Claims (6)

  1. 1. The bioreactor for stem cell exosome culture comprises a workbench (1), wherein a control panel (2) is fixedly connected to the front surface of the workbench (1), and is characterized in that a rotating disc (3) is installed on the top surface of the workbench (1), a driving motor (4) is arranged at the lower end of the rotating disc (3), the driving motor (4) is installed inside the workbench (1), an installation column (5) and a positioning rod (6) are sequentially and fixedly connected to the top surface of the rotating disc (3) from left to right, and an installation seat (7) is installed on the top surfaces of the installation column (5) and the positioning rod (6).
  2. 2. The bioreactor for culturing stem cell exosomes according to claim 1, wherein the lower end of the mounting seat (7) is provided with a groove which is mutually matched with the mounting column (5) and the positioning rod (6), and the top surface of the mounting seat (7) is provided with a culture cylinder (8).
  3. 3. The bioreactor for culturing stem cell exosomes according to claim 2, wherein a top plate (9) is installed on the top surface of the inner wall of the culture cylinder (8), and a motor (10) and a feeding cylinder (11) are installed on the top surface of the top plate (9) in sequence from left to right.
  4. 4. A bioreactor for culturing stem cell exosomes according to claim 3, wherein the output end of the mounting motor (10) is provided with a rotating shaft (12) penetrating through the top surface of the top plate (9), and the outer surface of the rotating shaft (12) is provided with a stirring assembly (13).
  5. 5. A bioreactor for culturing stem cell exosomes according to claim 3, wherein the outer surface of the feeding cylinder (11) is provided with an electromagnetic valve (14), and the lower end of the feeding cylinder (11) is provided with a blanking pipe (15) penetrating through the top surface of the top plate (9).
  6. 6. A bioreactor for culturing stem cell exosomes according to claim 3, characterized in that the interior of the feed cylinder (11) is provided with a sealing plug (16), and the top surface of the sealing plug (16) is provided with a sealing cover (17).

Description

Stem cell exosome bioreactor for culture Technical Field The utility model relates to the technical field of exosome preparation, in particular to a bioreactor for stem cell exosome culture. Background Stem cell exosomes are microvesicles with diameters of 40-100mm, and can transfer biomolecules such as lipids, carbohydrates, proteins, mRNA, miRNA, DNA and the like from one cell to another, so as to exchange genetic information and reprogram Cheng Suzhu cells, and perform intercellular communication. The stem cell exosomes have important biological functions of reducing apoptosis, reducing inflammatory reaction, promoting angiogenesis, inhibiting fibrosis, improving tissue repair potential and the like. In this regard, china application number CN219156893U discloses a bioreactor for culturing stem cell exosomes, which comprises a cell culture chamber and further comprises a base, wherein a gear ring is fixedly arranged on the base, a first gear and a driving mechanism for driving the first gear to rotate are rotatably arranged on the base in the gear ring, the rotation axis of the first gear is consistent with the height direction of the base, a second gear is rotatably arranged between the gear ring and the first gear, the second gear is respectively meshed with the first gear and the gear ring, and the cell culture chamber is fixedly arranged on the second gear. The application has the effect of improving insufficient mixing of the culture solution and the cells. However, in practical use, the culture chamber can only rotate through external rotation power when rotating, so that the culture solution and cells in the culture chamber can not be uniformly mixed, and the culture solution needs to be uniformly put in the culture chamber when being put in the culture chamber, so that improvement and improvement on the problems are the problems to be solved. Disclosure of utility model The utility model aims to provide a bioreactor for culturing stem cell exosomes, which solves the problems that in the prior art, when a culture chamber is rotated, only the culture chamber can be rotated by external rotation power, the culture solution and cells in the culture chamber cannot be uniformly mixed, and the culture solution needs to be uniformly put when being put. The bioreactor for stem cell exosome culture comprises a workbench, wherein a control panel is fixedly connected to the front surface of the workbench, a rotating disc is installed on the top surface of the workbench, a driving motor is arranged at the lower end of the rotating disc and is installed in the workbench, a mounting column and a positioning rod are sequentially and fixedly connected to the top surface of the rotating disc from left to right, and mounting seats are installed on the top surfaces of the mounting column and the positioning rod. Preferably, the lower extreme of mount pad is provided with the recess with erection column and locating lever looks adaptation each other, culture drum is installed to the top surface of mount pad. Preferably, a top plate is installed on the top surface of the inner wall of the culture cylinder, and an installation motor and a feeding cylinder are sequentially installed on the top surface of the top plate from left to right. Preferably, the output end of the installation motor penetrates through the top surface of the top plate to be provided with a rotating shaft, and the outer surface of the rotating shaft is provided with a stirring assembly. Preferably, the electromagnetic valve is arranged on the outer surface of the feeding cylinder, and the discharging pipe is arranged at the lower end of the feeding cylinder penetrating through the top surface of the top plate. Preferably, a sealing plug is arranged in the feeding barrel, and a sealing cover is arranged on the top surface of the sealing plug. Compared with the prior art, the utility model has the beneficial effects that: 1. This bioreactor that stem cell exosome was cultivateed usefulness, through the rolling disc that sets up, driving motor, the erection column, the locating lever, the mount pad, cultivate a section of thick bamboo, the roof, the installation motor, the feed cylinder, axis of rotation and stirring subassembly, when using, when culture fluid and cell are in the inside of cultivateing a section of thick bamboo, at first through starting driving motor, drive the rolling disc through driving motor and rotate, then fix a position the mount pad at the top surface of rolling disc through the design of erection column and locating lever, can drive the rotation that mount pad and cultivate a section of thick bamboo are stable through the rolling disc, then drive axis of rotation and stirring subassembly through starting the installation motor and rotate, thereby also can rotate culture fluid and cell inside the cultivation section of thick bamboo through the design of axis of rotation and stirring subassembly even, thereby can make culture f