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CN-224227089-U - High-flux cell culture and real-time monitoring device based on micro-fluidic chip

CN224227089UCN 224227089 UCN224227089 UCN 224227089UCN-224227089-U

Abstract

The utility model belongs to the technical field of microfluidics, and particularly discloses a high-throughput cell culture and real-time monitoring device based on a microfluidic chip, which comprises an installation folded plate, the microfluidic chip, two storage containers and a monitoring sensing assembly, wherein the installation folded plate comprises a transverse plate and a vertical plate arranged on one side edge of the transverse plate; the two storage containers are arranged on the vertical plate provided with the folded plate, and the bottom ends of the two storage containers are connected with the micro-flow output pipe. The micro-fluidic chip, the storage container, the micro-flow control pump, the monitoring sensing component and the like are integrated on the mounting folded plate to form a stable whole, and when the micro-fluidic chip is used, the observation of the cell culture process in the micro-fluidic chip is convenient.

Inventors

  • WU YAJIAN

Assignees

  • 西部医学科技集团有限公司

Dates

Publication Date
20260512
Application Date
20250603

Claims (6)

  1. 1. High-throughput cell culture and real-time monitoring device based on micro-fluidic chip, its characterized in that includes: the device comprises a mounting folded plate (1), a microfluidic chip (7), two storage containers (14) and a monitoring sensing assembly (13); The mounting folded plate (1) comprises a transverse plate and a vertical plate arranged on one side edge of the transverse plate, the microfluidic chip (7) is arranged on the upper surface of the transverse plate of the mounting folded plate (1), a feeding micro-channel (8) and an output micro-channel (9) are arranged in the microfluidic chip (7), two feeding micro-channels (8) are arranged, the tail ends of the two feeding micro-channels (8) are intersected to form a culture bin, and one end of the output micro-channel (9) is communicated with the culture bin; Two storage containers (14) are arranged on a vertical plate of the installation folded plate (1), the bottom ends of the two storage containers (14) are connected with a micro-flow output pipe (15), micro-flow control pumps (16) are arranged on the micro-flow output pipe (15), the other ends of the micro-flow output pipe (15) are respectively connected with two micro-channels (8), an installation support (12) is arranged on the vertical plate of the installation folded plate (1), and a monitoring sensing assembly (13) is arranged on the installation support (12).
  2. 2. The high-throughput cell culture and real-time monitoring device based on the microfluidic chip of claim 1, wherein the middle part of the transverse plate of the mounting folded plate (1) is provided with a supporting groove (2), a transparent supporting plate (3) is embedded in the supporting groove (2), the microfluidic chip (7) is positioned on the upper surface of the transparent supporting plate (3), and the lower surface of the mounting folded plate (1) is provided with a light supplementing device corresponding to the position of the transparent supporting plate (3).
  3. 3. The high-throughput cell culture and real-time monitoring device based on the microfluidic chip of claim 2, wherein the light supplementing device comprises a bearing plate (4) connected to the lower surface of the mounting folded plate (1) through a support column, and illuminating lamp beads (5) are equidistantly distributed on the upper surface of the bearing plate (4).
  4. 4. The high-throughput cell culture and real-time monitoring device based on the microfluidic chip of claim 3, wherein the two ends of the lower surface of the mounting folded plate (1) are provided with supporting strips (6), and the bottom ends of the supporting strips (6) are lower than the lower surface of the bearing plate (4).
  5. 5. The high-throughput cell culture and real-time monitoring device based on the microfluidic chip of claim 1, wherein the monitoring sensing component (13) comprises a fluorescence sensor and an image acquisition camera.
  6. 6. The high-throughput cell culture and real-time monitoring device based on the microfluidic chip of claim 1, wherein the upper surface of the transverse plate of the mounting folded plate (1) is provided with a liquid discharge concentration box (11), the output microchannel (9) is connected with an output tube (10), and the output tube (10) is connected with the liquid discharge concentration box (11).

Description

High-flux cell culture and real-time monitoring device based on micro-fluidic chip Technical Field The utility model relates to the technical field of microfluidics, in particular to a high-flux cell culture and real-time monitoring device based on a microfluidic chip. Background The microfluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes onto a micron-scale chip, and automatically completes the whole analysis process. The microfluidic chip is widely applied, for example, the growth state of cells cultured in the microfluidic chip is detected, but the monitoring mode is usually carried out through the combination of a plurality of devices in the cell culture process at present, so that the microfluidic chip is integrally dispersed and is not easy to operate. Disclosure of utility model The utility model aims to provide a high-throughput cell culture and real-time monitoring device based on a microfluidic chip, so as to solve the problems in the background technology. In order to achieve the purpose, the utility model provides the following technical scheme that the high-throughput cell culture and real-time monitoring device based on the microfluidic chip comprises: The device comprises a mounting folded plate, a microfluidic chip, two storage containers and a monitoring sensing assembly; The mounting folded plate comprises a transverse plate and a vertical plate arranged on one side edge of the transverse plate, the microfluidic chip is arranged on the upper surface of the transverse plate of the mounting folded plate, two feeding micro-channels and two output micro-channels are arranged in the microfluidic chip, the tail ends of the two feeding micro-channels are intersected to form a culture bin, and one end of the output micro-channel is communicated with the culture bin; The two storage containers are arranged on the vertical plate of the installation folded plate, the bottom ends of the two storage containers are connected with the micro-flow output pipe, the micro-flow control pumps are arranged on the micro-flow output pipe, the other ends of the two micro-flow output pipes are respectively connected with the two micro-channels, the vertical plate of the installation folded plate is provided with the installation support, and the installation support is provided with the monitoring sensing assembly. Preferably, a supporting groove is formed in the middle of the transverse plate of the installation folded plate, a transparent supporting plate is embedded in the supporting groove, the microfluidic chip is located on the upper surface of the transparent supporting plate, and a light supplementing device corresponding to the position of the transparent supporting plate is arranged on the lower surface of the installation folded plate. Preferably, the light supplementing device comprises a bearing plate connected to the lower surface of the mounting folded plate through a support column, and illuminating lamp beads are equidistantly distributed on the upper surface of the bearing plate. Preferably, the two ends of the lower surface of the mounting folded plate are respectively provided with a supporting bar, and the bottom ends of the supporting bars are lower than the lower surface of the bearing plate. Preferably, the monitoring sensing component comprises a fluorescence sensor and an image acquisition camera. Preferably, the upper surface of the transverse plate of the installation folded plate is provided with a liquid discharge centralized box, the output micro-channel is connected with an output pipe, and the output pipe is connected with the liquid discharge centralized box. Compared with the prior art, the utility model has the beneficial effects that: The micro-fluidic chip, the storage container, the micro-flow control pump, the monitoring sensing component and the like are integrated on the mounting folded plate to form a stable whole, and when the micro-fluidic chip is used, the observation of the cell culture process in the micro-fluidic chip is convenient. Drawings FIG. 1 is a schematic diagram of the structure of the present utility model; FIG. 2 is a schematic view of the structure of the mounting flap, the support slot, the transparent support plate and the carrier plate of the present utility model; Fig. 3 is a schematic structural diagram of the interior of the microfluidic chip of the present utility model. 1, Installing folded plates; 2, a supporting groove, 3, a transparent supporting plate, 4, a bearing plate, 5, an illuminating lamp bead, 6, a supporting bar, 7, a microfluidic chip, 8, a micro-channel supply, 9, an output micro-channel, 10, an output pipe, 11, a liquid discharge concentration box, 12, a mounting bracket, 13, a monitoring sensing component, 14, a storage container, 15, a microfluidic output pipe, 16 and a microfluidic control pump. Detail