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CN-224227090-U - Cell resuscitator culture shaking table

CN224227090UCN 224227090 UCN224227090 UCN 224227090UCN-224227090-U

Abstract

The utility model discloses a cell resuscitating culture table, which belongs to the technical field of cell resuscitating culture and comprises a box body, wherein a cover plate is rotatably connected to the box body, a clamping frame is arranged in the box body, a container is clamped in the clamping frame, a shaking device is fixedly connected in the box body, and a rotating device is fixedly connected to the upper surface of the shaking device. In the utility model, when the second motor drives the rotating plate to rotate, the movable rod drives the rotating block to do uniform circular motion in the shaking frame, in the process, the motion track of the rotating block is doubly constrained by the first connecting rod and the second connecting rod to form a composite motion track, meanwhile, the inclined plate generates periodic deflection along with the linkage action of the rotating block to drive the clamping frame to form a constant inclination angle of 15 degrees, and the unique mechanical linkage design ensures that the culture container keeps a specific inclination angle during the rotation motion, thereby forming a stable vortex field in the culture solution and obviously improving the mass transfer efficiency of a gas-liquid interface.

Inventors

  • WANG LONG
  • Chang Juyuan
  • YAO YUN
  • YANG SHENGLONG
  • DANG JIAJIA

Assignees

  • 上海多宁生物科技股份有限公司

Dates

Publication Date
20260512
Application Date
20250605

Claims (8)

  1. 1. The utility model provides a cell resuscitator culture shaking table, includes box (1), its characterized in that, rotate on box (1) and be connected with apron (2), be equipped with clamping frame (3) in box (1), clamping frame (3) inside callipers are equipped with container (4), fixedly connected with shakes device (6) in box (1), shake device (6) upper surface fixedly connected with rotary device (5); The shaking device (6) comprises a frame body (601) and a second motor (602), wherein the output end of the second motor (602) is fixedly connected with a rotating plate (603), a movable rod (604) is arranged in the rotating plate (603), a rotating block (605) is fixedly connected to the movable rod (604), a first connecting rod (606) is symmetrically connected in the frame body (601), a second connecting rod (608) is symmetrically arranged in the frame body (601), and the second connecting rod (608) is rotationally connected in the rotating block (605).
  2. 2. The cell resuscitation table according to claim 1, wherein the shaking device (6) further comprises a shaking frame (607) arranged in the frame body (601), and the shaking frame (607) and the first connecting rod (606) form a rotational connection fit.
  3. 3. The cell resuscitation table according to claim 1, wherein the upper surface of the rotating block (605) is fixedly connected with an inclined plate (609), and the inclination angle is 15 degrees.
  4. 4. A cell resuscitation table according to claim 3, wherein said rotation means (5) comprises four casings (501) and a first motor (505), said four casings (501) being fixedly connected to the upper surface of said inclined plate (609), a slide bar (502) being provided in said casings (501), said slide bar (502) being provided with a pull-back spring (503) being provided outside said slide bar (502).
  5. 5. The cell resuscitation table according to claim 4, wherein one end of the pull-back spring (503) is fixedly connected to the outside of the slide bar (502), and the other end of the pull-back spring (503) is fixedly connected to the upper surface of the casing (501).
  6. 6. The cell resuscitation table according to claim 4, wherein the upper surfaces of the four slide bars (502) are fixedly connected with the same connecting plate (504).
  7. 7. The cell resuscitation table according to claim 4, wherein the inner diameter of the housing (501) is slightly larger than the cross section of the sliding rod (502), and a sliding connection fit is formed between the two.
  8. 8. The cell resuscitation table according to claim 4, wherein the first motor (505) is connected in the connecting plate (504) in a penetrating manner, and an output end of the first motor (505) is fixedly connected to a lower surface of the clamping frame (3).

Description

Cell resuscitator culture shaking table Technical Field The utility model belongs to the technical field of cell resuscitating culture, and particularly relates to a cell resuscitating culture shaking table. Background The cell resuscitating culture is to take out frozen cells from liquid nitrogen or refrigerator at-80 deg.c, fast defrost and transfer the frozen cells to preheated culture medium to restore activity and proliferation capacity, to eliminate freeze protecting agent (DMSO), to re-suspend the thawed cells in fresh culture medium, to culture in culture bottle or culture dish, to culture in constant temperature incubator with 37 deg.c and 5% CO 2, to culture the suspended cells or to expand culture, to shake the cells in shaking table with constant rotation speed of 100-150-rpm to promote the homogeneous distribution of nutrients and gas exchange in the culture medium and avoid cell deposition. In the prior art, a single rotation mode of a traditional shaking table can only drive culture solution to generate basic reciprocating motion, effective fluid mixing is difficult to form, a uniform mass transfer environment cannot be established in a culture system due to the lack of a multi-dimensional dynamic mixing mechanism, so that gas-liquid exchange efficiency is limited, especially in the cell culture process, along with the change of physicochemical properties of the culture solution, the phenomenon of insufficient mixing is easier to occur in the simple movement mode, local dissolved oxygen and uneven nutrition distribution are caused, the growth state and metabolic activity of cells are influenced, and compared with the homogenized mixing effect of a bioreactor realized by optimizing a flow field design, the traditional shaking table has obvious defects in mass transfer efficiency, so that the application performance of the traditional shaking table in a strict high-density culture or virus amplification process is restricted to a certain extent. Based on the above, the utility model designs a cell resuscitating culture table to solve the above problems. Disclosure of utility model The utility model aims to solve the problems that in the prior art, a single rotation mode of a traditional shaking table only can drive culture solution to generate basic reciprocating motion, effective fluid mixing is difficult to form, and a uniform mass transfer environment cannot be established in a culture system due to the lack of a multi-dimensional dynamic mixing mechanism, so that gas-liquid exchange efficiency is limited. In order to achieve the above purpose, the present utility model adopts the following technical scheme: The cell resuscitating culture table comprises a box body, wherein a cover plate is rotatably connected to the box body, a clamping frame is arranged in the box body, a container is clamped in the clamping frame, a shaking device is fixedly connected in the box body, and a rotating device is fixedly connected to the upper surface of the shaking device; the shaking device comprises a frame body and a second motor, wherein the output end of the second motor is fixedly connected with a rotating plate, a movable rod is arranged in the rotating plate, a rotating block is fixedly connected to the movable rod, first connecting rods are symmetrically connected in the frame body, second connecting rods are symmetrically arranged in the frame body, and the second connecting rods are rotationally connected in the rotating block. As a further description of the above technical solution: The shaking device further comprises a shaking frame arranged in the frame body, and the shaking frame and the first connecting rod form rotary connection fit. As a further description of the above technical solution: the upper surface of the rotating block is fixedly connected with an inclined plate, and the inclination angle is 15 degrees. As a further description of the above technical solution: The rotating device comprises four sleeves and a first motor, the four sleeves are fixedly connected to the upper surface of the inclined plate, a sliding rod is arranged in the sleeve, and a pull-back spring is sleeved outside the sliding rod. As a further description of the above technical solution: One end of the pull-back spring is fixedly connected outside the sliding rod, and the other end of the pull-back spring is fixedly connected to the upper surface of the casing. As a further description of the above technical solution: the upper surfaces of the four sliding rods are fixedly connected with the same connecting plate. As a further description of the above technical solution: the inner diameter of the shell is slightly larger than the cross section of the sliding rod, and sliding connection fit is formed between the two. As a further description of the above technical solution: the first motor is connected in the connecting plate in a penetrating way, and the output end of the first motor is fixedly connected to t