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CN-224227091-U - Cell tolerance testing device

CN224227091UCN 224227091 UCN224227091 UCN 224227091UCN-224227091-U

Abstract

The utility model discloses a cell tolerance testing device, which belongs to the technical field of cell culture and comprises a shell, wherein a switch door is arranged on one side of the shell, a liquid injection device is fixedly connected to the shell, a receiving device and a rotating device are arranged in the shell, the liquid injection device comprises a first motor and an injection cylinder, the output end of the first motor is fixedly connected with a piston, and the piston is arranged in the injection cylinder. When the virus inoculation liquid is needed to be connected into the cell liquid, the virus inoculation liquid is only needed to be injected through the one-way water inlet valve, in the operation process, the first motor is started, the motor drives the piston to axially move along the inside of the injection cylinder, a certain pressure is applied to the virus inoculation liquid in the injection cylinder, the virus inoculation liquid is discharged through the liquid outlet nozzle under the action of the pressure, then flows into the flow guide nozzle, and finally is injected into the measuring cup corresponding to the lower part, so that pollution-free and quantitative injection of the virus inoculation liquid is realized.

Inventors

  • WANG LONG
  • YU ZEHUA
  • YAO YUN
  • DANG JIAJIA
  • YANG SHENGLONG

Assignees

  • 上海多宁生物科技股份有限公司

Dates

Publication Date
20260512
Application Date
20250605

Claims (8)

  1. 1. The cell tolerance testing device comprises a shell (1) and is characterized in that a switch door (2) is arranged on one side of the shell (1), a liquid injection device (3) is fixedly connected to the shell (1), and a receiving device (4) and a rotating device (5) are arranged in the shell (1); the priming device (3) includes first motor (305) and injection tube (303), the output fixedly connected with piston (304) of first motor (305), piston (304) set up in injection tube (303), fixedly connected with liquid outlet (306) under injection tube (303), through-connection has one-way water intaking valve (307) in injection tube (303).
  2. 2. The cell tolerance test device according to claim 1, wherein the priming device (3) further comprises a fixing plate (301), the fixing plate (301) is fixedly connected to the upper surface of the housing (1), and the injection cylinder (303) is fixedly connected to the front surface of the fixing plate (301).
  3. 3. The cell tolerance test device according to claim 2, wherein the front surface of the fixing plate (301) is fixedly connected with a locking plate (302), and the first motor (305) is fixedly connected to the upper surface of the locking plate (302).
  4. 4. The cell tolerance test device according to claim 1, wherein the receiving device (4) comprises four cross bars (401), the four cross bars (401) are symmetrically arranged on the inner wall of the shell (1), a disc (402) is fixedly connected to the four cross bars (401), and a flow guide nozzle (403) is connected to the disc (402) in a penetrating manner.
  5. 5. A cell tolerance test device according to claim 4, wherein the cross-section of the flow nozzle (403) has a larger diameter than the cross-section of the liquid outlet nozzle (306), the flow nozzle (403) being positioned in correspondence with the liquid outlet nozzle (306).
  6. 6. The cell tolerance test device according to claim 1, wherein the rotating device (5) comprises four limiting rods (501) and a mounting plate (503), the four limiting rods (501) are symmetrically mounted on the inner wall of the shell (1), one end of each limiting rod (501) is rotatably connected with a roller (502), and the four rollers (502) are lapped with the same rotary table (505).
  7. 7. The cell tolerance test device according to claim 6, wherein the mounting plate (503) is fixedly connected in the housing (1), and the mounting plate (503) is fixedly connected with a second motor (504).
  8. 8. The cell tolerance test device according to claim 7, wherein the output end of the second motor (504) is fixedly connected to the lower surface of the turntable (505), and a plurality of measuring cups (506) are installed in the turntable (505).

Description

Cell tolerance testing device Technical Field The utility model belongs to the technical field of cell culture, and particularly relates to a cell tolerance testing device. Background Cell culture and virus process research are one of the core fields of modern biopharmaceuticals, vaccine research and virology research, and aim to realize efficient and controllable virus production process by optimizing cell growth environment and virus proliferation conditions, the research field is widely applied to multiple directions of vaccine preparation, virus vector production, antiviral drug evaluation, molecular virology research and the like, in virus vaccine production, the selection and culture mode (such as adherence culture or suspension culture) of host cells have decisive influence on virus replication efficiency and product quality, and the infection sensitivity and replication supporting capacity of the mammalian cells (such as Vero, BHK-21, MDCK cells and the like) on specific virus strains and the tolerance degree on virus load directly determine the stability and economy of the process. In the prior art, a virus inoculation liquid is usually required to be connected into a cell culture system for cell tolerance test so as to evaluate the growth state and metabolic response of cells under the virus infection condition, however, in the actual operation process, the virus inoculation liquid is usually added in a cover opening or exposure type sample adding mode, so that the system is easily exposed to the air environment, the pollution risk is increased, and the virus inoculation efficiency and the stability of cell response are influenced due to the fluctuation of gas components, the interference of microorganisms or the change of temperature and humidity, and uncontrollable interference is further generated on experimental results, so that the accuracy and repeatability of the test are influenced. Based on this, the present utility model has devised a cell tolerance test device to solve the above-mentioned problems. Disclosure of utility model The utility model aims to solve the problems that in the prior art, virus inoculation liquid is usually required to be connected into a cell culture system for carrying out cell tolerance test so as to evaluate the growth state and metabolic response of cells under the virus infection condition, however, in the actual operation process, the virus inoculation liquid is usually added in a cover opening or exposure type sample adding mode, so that the system is easily exposed to the air environment, the pollution risk is increased, and the virus inoculation efficiency and the stability of cell response are possibly influenced due to the fluctuation of gas components, the interference of microorganisms or the change of temperature and humidity. In order to achieve the above purpose, the present utility model adopts the following technical scheme: The cell tolerance testing device comprises a shell, wherein one side of the shell is provided with a switch door, the shell is fixedly connected with a liquid injection device, and a receiving device and a rotating device are arranged in the shell; The liquid injection device comprises a first motor and a syringe, wherein the output end of the first motor is fixedly connected with a piston, the piston is arranged in the syringe, a liquid outlet nozzle is fixedly connected under the syringe, and a one-way water inlet valve is connected in the syringe in a penetrating manner. As a further description of the above technical solution: The priming device still includes the fixed plate, fixed plate fixed connection is at the upper surface of casing, injection tube fixed connection is in the front of fixed plate. As a further description of the above technical solution: the front fixedly connected with locking plate of fixed plate, first motor fixed connection is at the upper surface of locking plate. As a further description of the above technical solution: the receiving device comprises four cross bars, the four cross bars are symmetrically arranged on the inner wall of the shell, the four cross bars are fixedly connected with discs, and the guide nozzles are connected in the discs in a penetrating manner. As a further description of the above technical solution: The diameter of the cross section of the flow guiding nozzle is larger than that of the cross section of the liquid outlet nozzle, and the positions between the flow guiding nozzle and the liquid outlet nozzle are corresponding. As a further description of the above technical solution: The rotating device comprises four limiting rods and a mounting plate, wherein the four limiting rods are symmetrically arranged on the inner wall of the shell, one end of each limiting rod is rotatably connected with a roller, and the four rollers are lapped with the same rotary table. As a further description of the above technical solution: The mounting plate is fixedly connected in the