CN-224230486-U - Program cooling instrument

CN224230486UCN 224230486 UCN224230486 UCN 224230486UCN-224230486-U

Abstract

The application relates to the technical field of biomedical science, and particularly discloses a program cooling instrument which comprises a sample cavity, a Stirling refrigerator, a thermoelectric refrigerating device, a heat exchange system and a control system. The sample cavity is positioned at the center of the top of the program cooling instrument and is used for storing biological samples, the sample cavity carries out graded refrigeration through a thermoelectric refrigeration device and a Stirling refrigerator double refrigeration source, the double refrigeration source realizes smooth transition through a control system, and the functions of heat transfer and heat dissipation are realized through a heat exchange system in the refrigeration process. The purpose of this patent is to solve the problem that current single refrigeration source performance limitation under wide temperature range. The method is mainly used for gradually cooling the biological sample in a specific temperature area according to a preset program.

Inventors

Xue Mingmei
ZHANG RUI
XU FANGMIN

Assignees

贵州北科生物科技有限公司

Dates

Publication Date: 20260512
Application Date: 20250606

Claims (7)

1. A program cooling instrument is characterized by comprising a sample cavity, a Stirling refrigerator, a thermoelectric refrigerating device and a heat exchange system, wherein the sample cavity is positioned at the center of the top of the program cooling instrument and is used for storing biological samples, the sample cavity is subjected to graded refrigeration through a thermoelectric refrigerating device and a Stirling refrigerator dual refrigeration source, the thermoelectric refrigerating device is responsible for refrigerating above 60 ℃ below zero, the Stirling refrigerator is responsible for refrigerating between 60 ℃ below zero and 196 ℃ below zero, the heat exchange system comprises a water cooling system and an air cooling system, the water cooling system is used for heat dissipation of a Stirling hot end, the air cooling system comprises a fan and a radiator shared by the water cooling system, and the fan is arranged on the back of the program cooling instrument.
2. The program cooling instrument of claim 1, wherein the Stirling refrigerator comprises a Stirling cold end, a Stirling hot end and a refrigerator, the Stirling cold end is closely attached to the bottom of the sample cavity, the Stirling hot end dissipates heat through a water cooling system, and the refrigerator is a mechanical refrigerator driven by electricity.
3. The program cooling instrument of claim 1, wherein the thermoelectric refrigeration device comprises a TEC cold end and a TEC hot end, the TEC cold end is clung to the bottom of the sample cavity, and the TEC hot end dissipates heat through an air cooling system.
4. The program cooling instrument according to claim 1, wherein the water cooling system comprises a radiator, a water pipe, a water pump, a water tank and a water cooling block, wherein the radiator adopts a fin radiator and is fixed at the hot end of the TEC, the water pipe is spirally wound on the radiator, the water cooling block is a metal block with a water channel in the water pipe and is tightly attached to the hot end of the Stirling engine, the water tank is used for storing circulating liquid, the circulating liquid flows after being pressurized by the water pump, and the water pipe is used for connecting the water pump, the water cooling block and the water tank and is used for enabling the circulating liquid to flow in a closed channel without leakage.
5. The apparatus of claim 1, wherein the heat exchange system further comprises a heat conduction bridge, the heat conduction bridge is a T-shaped alumina block embedded in the bottom of the sample cavity, the horizontal segment is connected with the TEC cold end, and the vertical end is connected with the Stirling cold end.
6. The apparatus of claim 1, wherein the upper portion of the apparatus has a sealed viewing window.
7. The program cooling instrument of claim 1, further comprising a control system, wherein the control system comprises a control screen and a temperature sensor, the control screen is arranged on the upper surface of the program cooling instrument, and the temperature sensor is respectively arranged in the sample cavity, the TEC cold end, the TEC hot end, the Stirling cold end and the Stirling hot end.

Description

Program cooling instrument Technical Field The utility model relates to the technical field of biomedical treatment, in particular to a program cooling instrument. Background The program cooling instrument is a key device for low-temperature preservation of biological samples, and can be used for gradually cooling the samples in a specific temperature range according to a preset program by precisely controlling the cooling rate, so that the formation of ice crystals and osmotic pressure damage in cells are reduced, and the cell recovery rate is remarkably improved. Especially in the preservation of living cells, tissues and biomacromolecules, the program cooling instrument needs to realize stable and controllable gradient cooling in a wide temperature range (such as normal temperature to 196 ℃ below zero) so as to meet the differential requirements of different biological samples on cooling curves. In the prior art, a program cooling instrument mainly depends on a single refrigeration source to realize low-temperature control. For example, CN212057877U discloses a liquid nitrogen-free program cooling instrument based on a stirling cooler, which realizes deep low-temperature refrigeration (196 ℃ below zero) through stirling cycle, avoids the problem of liquid nitrogen dependence, but has limitations in cooling efficiency and energy consumption control in medium-high temperature sections (e.g. above 60 ℃ below zero). On the other hand, CN205536658U provides a thermoelectric refrigeration cooling device, which utilizes the Peltier effect to combine with a semiconductor material to realize accurate temperature control, is energy-saving and environment-friendly, but the refrigeration capacity is limited by the characteristics of the semiconductor material, and is difficult to cover the ultralow temperature requirement. Although the above techniques have advantages, a single refrigeration source is adopted, and the problem of adaptability of refrigeration performance in a wide temperature range is not solved. The technical pain of the current program cooling instrument is that a single refrigeration source is limited by the working principle, and the requirements of high-efficiency refrigeration, accurate temperature control and low energy consumption are difficult to meet in a wide temperature range. For example, stirling refrigerators are excellent in performance in a very low temperature section, but the thermodynamic efficiency is reduced in a medium-high Wen Duanyin section to increase energy consumption, while thermoelectric refrigerating devices are flexible in temperature control in a medium-low temperature section, but cannot meet the requirements of deep low temperature. Therefore, a program cooling device capable of integrating advantages of multiple refrigeration sources and realizing seamless connection in a wide temperature range is needed to improve the overall performance and the application range. Disclosure of utility model Aiming at the defects of the prior art, the utility model provides a program cooling instrument which solves the problem of performance limitation of the existing single refrigeration source in a wide temperature range. In order to solve the problems, the technical scheme includes that the program cooling instrument comprises a sample cavity, a Stirling refrigerator, a thermoelectric refrigerating device and a heat exchange system, wherein the sample cavity is positioned in the center of the top of the program cooling instrument and is used for storing biological samples, the sample cavity is subjected to graded refrigeration through a thermoelectric refrigerating device and a Stirling refrigerator double refrigerating source, the thermoelectric refrigerating device is used for refrigerating above 60 ℃ below zero, the Stirling refrigerator is used for refrigerating between 60 ℃ below zero and 196 ℃ below zero, the heat exchange system comprises a water cooling system and an air cooling system, the water cooling system is used for heat dissipation of a Stirling hot end, the air cooling system comprises a fan and a radiator shared by the fan and the water cooling system, and the fan is arranged on the back of the program cooling instrument. The technical principle of the scheme is that 1, the thermoelectric refrigerating device is based on the Peltier effect, when direct current passes through a loop formed by two different conductors, one end absorbs heat and the other end releases heat to form a temperature difference, the thermoelectric refrigerating device has the advantages of quick response, accurate temperature control and low manufacturing cost, but also has the defects of low thermoelectric conversion efficiency, limited refrigerating power and difficulty in realizing extremely low-temperature refrigeration, 2, the Stirling refrigerator is based on reverse Stirling circulation, and realizes heat transfer from a cold temperature area to a high-temperature area through t