CN-224231416-U - Reflux mechanism for high-temperature pretreatment of trace biological samples

Abstract

The utility model discloses a reflux mechanism for high-temperature pretreatment of a trace biological sample, which relates to the technical field of pretreatment of biological samples, and comprises a plurality of reflux caps, a plurality of test tubes and a heater, wherein the heater consists of a shell and a heating plate, the heating plate is provided with a group of uniformly arranged heating holes, and the heating plate is a porous metal heating plate; each test tube is inserted into the corresponding heating hole, and the diameter of the outer wall of each test tube is matched with the inner diameter of the corresponding heating hole. The utility model replaces the traditional plug with the reflux cap made of glass material, is suitable for high-temperature reaction of trace samples, can meet the requirement of simultaneous treatment of a plurality of samples, is suitable for high-temperature reaction of different organic solvents at a temperature close to or higher than the boiling point of the organic solvents, ensures that the samples have a constant reaction solvent volume in long-time high-temperature reaction, and effectively improves the high-temperature reaction effect of the solvents and biological samples.

Inventors

• ZHAO FURONG
• SUN HONGZHI

Assignees

• 锦州医科大学附属第一医院

Dates

Publication Date

20260512

Application Date

20250530

Claims (5)

1. 1. The reflux mechanism for high-temperature pretreatment of the trace biological samples comprises a plurality of reflux caps (1), a plurality of test tubes (2) and a heater, and is characterized in that the heater consists of a shell (5) and a heating plate (4), the heating plate (4) is provided with a group of uniformly arranged heating holes (3), and the heating plate (4) is a porous metal heating plate; Each test tube (2) is inserted into the corresponding heating hole (3), and the diameter of the outer wall of each test tube (2) is matched with the inner diameter of the corresponding heating hole (3); each reflux cap (1) covers the upper end of the corresponding test tube (2).
2. 2. A reflow mechanism for high temperature pretreatment of micro biological samples according to claim 1, wherein each of the reflow cap (1) and the test tube (2) is made of glass.
3. 3. A reflow mechanism for high temperature pretreatment of micro-biological samples according to claim 1, wherein the difference between the inner diameter of each reflow cap (1) and the outer diameter of each test tube (2) is <1 mm.
4. 4. A reflow mechanism for high temperature pretreatment of micro biological samples according to claim 1, wherein the height of each reflow cap (1) is between 1/5 and 1/3 of the height of each test tube (2).
5. 5. A reflow mechanism for high temperature pretreatment of micro biological samples according to claim 1, wherein the hole depth of each heating hole (3) is < 1/3 of the height of the test tube (2).

Description

Reflux mechanism for high-temperature pretreatment of trace biological samples Technical Field The utility model relates to the technical field of biological sample pretreatment, in particular to a reflux mechanism for high-temperature pretreatment of a trace biological sample. Background Biological samples have important significance in medical research, and can help understand life process, disease mechanism, drug research and development and the like. Common biological samples comprise blood, tissue slices, urine, feces and the like, and because the biological samples have high complexity, the chemical components in the biological samples are rich and various, the components are mutually interwoven together to form a complex chemical system, meanwhile, a large number of interferents exist in the samples, the interferents have various types and different properties, interfere the detection of target compounds, reduce the sensitivity and even damage instrument elements, and pretreatment is usually required to remove impurities and concentrate the target substances and adjust the properties of the samples, so that the detection sensitivity is improved, instrument equipment is protected, the separation effect is optimized, and the accuracy and the reliability of analysis results are ensured. Along with the development of analysis technology, trace biological samples can meet analysis requirements, in the traditional high-temperature pretreatment (such as derivatization reaction, hydrolysis reaction and the like), researchers usually adopt a mode of sealing a reaction container (such as a glass test tube or a reaction bottle) by a plug to prevent solvent volatilization under high-temperature conditions, but the treatment mode has obvious potential safety hazards that when a reaction system is heated, the internal pressure of the sealed container is rapidly increased due to solvent vaporization, potential safety hazards such as plug ejection or container bursting are extremely easy to occur, otherwise, if non-sealing treatment is adopted, trace solvent can be rapidly volatilized and lost under the high-temperature condition which is close to or exceeds the boiling point of the solvent, so that the concentration and the composition of the reaction system can be changed, and even the conversion efficiency and the final detection result of a target object can be seriously influenced. Disclosure of utility model In order to solve the problems, namely the problems proposed by the background technology, the utility model provides a reflux mechanism for high-temperature pretreatment of a trace biological sample, which comprises a plurality of reflux caps, a plurality of test tubes and a heater, wherein the heater consists of a shell and a heating plate, the heating plate is provided with a group of uniformly arranged heating holes, and the heating plate is a porous metal heating plate; Each test tube is inserted into the corresponding heating hole, and the diameter of the outer wall of each test tube is matched with the inner diameter of the corresponding heating hole; each reflux cap covers the upper end of the corresponding test tube. The utility model further provides that each reflux cap and each test tube are made of glass. The utility model is further arranged such that the difference between the inner diameter of each of said reflow caps and the outer diameter of each of said test tubes is < 1mm. The utility model is further arranged that the height of each reflux cap is between 1/5 and 1/3 of the height of each test tube. The utility model is further arranged that the hole depth of each heating hole is less than 1/3 of the height of the test tube. The utility model is further arranged that the heating hole of the heater heats the tube wall in a fitting way. The utility model is further provided that the heater is a metal heater with a vibration function (for example, if a solvent is poured into a reflux cap 1 on the inner cover of a test tube 2 in the process of conducting fatty acid hydrolysis reaction, the solvent is inserted into a metal heating hole which is heated to a preset temperature of 90 ℃ and then vibration at 1500rpm is needed to promote the reaction). In the utility model, the upper limit of the high temperature condition is set according to the type of the heater, the heating temperature and the temperature required by the solvent test are set, and the larger the temperature difference is, the longer the heating time is, the smaller the temperature difference is, and the shorter the heating time is, taking into consideration the temperature difference between the target heating temperature and the boiling point of the solvent. The utility model is further arranged that the specification of the test tube is in a range of being applicable to organic solvents such as methanol, ethanol, acetonitrile, ethyl acetate and the like with the volume of 200 microliters to 2000 microliters, and the organic