CN-224231686-U - Five-way three-electrode electrochemiluminescence detector

CN224231686UCN 224231686 UCN224231686 UCN 224231686UCN-224231686-U

Abstract

The utility model discloses a five-path three-electrode electrochemiluminescence detector which comprises an electrochemiluminescence chip and a constant potential excitation device, wherein the electrochemiluminescence chip comprises an electrode slice and a sample adding slice, the front surface of the electrode slice is provided with a shared counter electrode, a first working electrode, a first reference electrode, a second working electrode and a second reference electrode, the back surface of the electrode slice is provided with a driving electrode of the shared counter electrode, the shared counter electrode is connected with the driving electrode, the sample adding slice covers the shared counter electrode, the first working electrode, the first reference electrode, the second working electrode and the second reference electrode, the bottom ends of the first working electrode, the second reference electrode and the driving electrode are used as electric contact areas, the shared counter electrode, the first working electrode and the first reference electrode or the sample adding slice corresponding to the first working electrode or the second working electrode and the second reference electrode form a first reaction tank and a second reaction tank, and the constant potential excitation device is connected with the electric contact areas to excite the first reaction tank and the second reaction tank to generate electrochemiluminescence reaction.

Inventors

ZHANG CHUNSUN
ZHONG MO

Assignees

华南师范大学
华南师大(清远)科技创新研究院有限公司
好希生物技术(清远)有限公司

Dates

Publication Date: 20260512
Application Date: 20250408

Claims (10)

1. The five-path three-electrode electrochemiluminescence detector is characterized by comprising an electrochemiluminescence chip and a constant potential excitation device, wherein the electrochemiluminescence chip comprises an electrode plate, a sample adding plate, an upper cover and a lower cover, the electrode plate and the sample adding plate are arranged between the upper cover and the lower cover, a shared counter electrode, a first working electrode, a first reference electrode, a second working electrode and a second reference electrode are arranged on the front surface of the electrode plate, a driving electrode of the shared counter electrode is arranged on the back surface of the electrode plate, the shared counter electrode is connected with the driving electrode, and the sample adding plate covers the shared counter electrode, the first working electrode, the first reference electrode, the second working electrode and the second reference electrode; the bottom ends of the first working electrode, the first reference electrode, the second working electrode and the second reference electrode and the bottom end of the driving electrode are used as electric contact areas, a first reaction tank is formed by sharing the first side of the counter electrode, the first side of the sample adding sheet corresponding to the first working electrode and the first reference electrode, a second reaction tank is formed by sharing the second side of the counter electrode, the second side of the sample adding sheet corresponding to the second working electrode and the second reference electrode, and a constant potential excitation device is connected with the electric contact areas to excite the first reaction tank and the second reaction tank to generate electrochemiluminescence reaction; The constant potential excitation device comprises a power supply unit, a constant potential circuit, an external interface circuit, a WiFi circuit, a control unit, a relay circuit, an OLED display screen, a reset circuit, a serial port circuit, a signal generation circuit and a key circuit which are integrated on a circuit board, wherein the constant potential circuit, the external interface circuit, the WiFi circuit, the control unit, the relay circuit, the OLED display screen, the reset circuit, the serial port circuit, the signal generation circuit and the key circuit are respectively connected with the power supply unit, the constant potential circuit, the external interface circuit, the WiFi circuit, the relay circuit, the OLED display screen, the reset circuit, the serial port circuit, the signal generation circuit and the key circuit are respectively connected with the control unit, the signal generation circuit is connected with the constant potential circuit, and the power supply unit is connected with an external power supply.
2. The five-way three-electrode electrochemiluminescence detector according to claim 1, wherein the power supply unit comprises a +5v to +3.3v buck circuit, +5v to +12v boost circuit, +5v to-12v buck circuit, +12v to +5v buck circuit and +12v to +5.21v buck circuit, the +5v to +3.3v buck circuit providing power for the control unit, relay circuit, signal generation circuit, OLED display screen, serial circuit and WiFi circuit; The +5V-to-12V step-up circuit provides power for the signal generating circuit and the constant potential circuit, and provides power for the +12V-to-5V step-down circuit and the +12V-to-5.21V step-down circuit, the +5V-to-12V step-down circuit provides power for the signal generating circuit and the constant potential circuit, and the +12V-to- +5V step-down circuit and the +12V-to- +5.21V step-down circuit provide power for the signal generating circuit.
3. The five-way three-electrode electrochemiluminescence detector according to claim 2, wherein +5v to +3.3v step-down circuit converts +5v voltage of an external power supply into +3.3v voltage through a linear voltage stabilizing chip AMS1117-3.3, +3v voltage indicates the working state of the circuit through a 4.7kΩ resistor for current limiting driving indicator lamp, and the circuit configuration is 22 μf and 100nF decoupling capacitors for filtering.
4. The five-way three-electrode electrochemiluminescence detector according to claim 2, wherein a +5v-to- +12v boost circuit boosts +5v voltage of an external power supply through a direct current conversion chip MT3608, the boosted voltage signal is converted into a +12v output signal by a linear voltage stabilizer 78L12, the +12v output signal is isolated into an analog +12v voltage signal and a digital +12v voltage signal through a 0 Ω resistor, the analog +12v voltage signal provides a positive voltage power supply for a signal generating circuit and a potentiostat circuit, and the digital +12v voltage signal provides a positive voltage power supply for a +12v-to- +5v step-down circuit and a +12v-to- +5.21v step-down circuit; The +5V-to-12V voltage reduction circuit converts +5V voltage of an external power supply into a negative voltage signal through an asynchronous voltage reduction converter HT7463A, the negative voltage signal is converted into a-12V output signal through a linear voltage stabilizer 79L12, the-12V output signal is isolated into an analog-12V voltage signal and a digital-12V voltage signal through a 0 omega resistor, and the analog-12V voltage signal provides a negative voltage power supply for the signal generation circuit and the constant potential circuit.
5. The five-way three-electrode electrochemiluminescence detector of claim 4, wherein the +12v to +5v step-down circuit converts a digital +12v voltage signal to a 5V voltage through a voltage reference chip REF195GSZ to provide a regulated power supply for the signal generating circuit; The +12V-to- +5.21V step-down circuit converts a digital +12V voltage signal into +5.21V voltage through a linear voltage stabilizer LM317LF, and the +5.21V voltage drives an indicator lamp LED9 to emit light through a 10KΩ resistor current limiting to indicate the working state of the circuit.
6. The five-way three electrode electrochemiluminescence detector of claim 2, wherein the external interface circuit comprises a +3.3v interface, +12v interface, -12v interface, +5v interface, +5.21v interface, a shared counter electrode interface, a first working electrode interface, a first reference electrode interface, a second working electrode interface, and a second reference electrode interface; The +3.3V interface is connected with +5V to +3.3V step-down circuit output end, +12V interface is connected with +5V to +12V step-up circuit output end, -12V interface is connected with +5V to +12V step-down circuit output end, +5V interface is connected with +12V to +5V step-down circuit output end, +5.21V interface is connected with +12V to +5.21V step-down circuit output end, share counter electrode interface, first working electrode interface, first reference electrode interface, second working electrode interface and second reference electrode interface are connected with driving electrode, first working electrode, first reference electrode, second working electrode and second reference electrode respectively.
7. The five-way three-electrode electrochemiluminescence detector according to claim 1, wherein the potentiostatic circuit comprises two voltage followers OPA604, an operational amplifier OP07 and a power amplifier BUF634, the two voltage followers OPA604 are used for maintaining constant the potential between the first reference electrode and the first working electrode or between the second reference electrode and the second working electrode, the operational amplifier OP07 provides an excitation signal for the shared counter electrode, and the power amplifier BUF634 amplifies the excitation signal output by the operational amplifier OP07 again.
8. The five-way three-electrode electrochemiluminescence detector according to claim 1, wherein the control unit comprises an STM32F103CBT6 chip, a clock circuit, a starting circuit and a decoupling circuit, the clock circuit adopts a crystal oscillator to provide an operation clock signal for the STM32F103CBT6 chip, two ends of the crystal oscillator are connected with capacitors for filtering, the starting circuit adopts pins to be respectively connected with a BOOT0 pin and a BOOT1 pin of the STM32F103CBT6 chip in series through resistors, and the decoupling circuit adopts a plurality of capacitors to connect a 3.3V supply voltage with the ground end of the STM32F103CBT6 chip.
9. The five-way three-electrode electrochemiluminescence detector according to claim 1, wherein the serial port circuit adopts a USB-to-TTL serial port chip, the reset circuit adopts a low-level reset mode, the relay circuit adopts an NPN transistor and a PNP transistor as switches, and the OLED display screen is used for displaying the state of a constant potential excitation device and the magnitude of an excitation voltage value.
10. The five-way three electrode electrochemiluminescence detector of claim 1, wherein the signal generating circuit comprises a digital-to-analog conversion chip DAC8831 and an operational amplifier OPA277, and the digital signal output by the microcontroller is converted into an analog signal by the digital-to-analog conversion chip DAC8831 and the operational amplifier OPA277 and sent to the potentiostatic circuit.

Description

Five-way three-electrode electrochemiluminescence detector Technical Field The utility model relates to the technical field of electrochemiluminescence detection, in particular to a five-way three-electrode electrochemiluminescence detector. Background Electrochemical luminescence (Electrochemiluminescence, ECL) technology has been widely used in biomedical, environmental monitoring, food safety, etc. fields as an analytical method with high sensitivity and high selectivity. Electrochemical luminescence detection systems generally consist of a detection unit, an excitation unit, a photodetection unit and a data processing unit. In conventional three-electrode electrochemiluminescence detection systems, the detection unit typically comprises a working electrode, a reference electrode, and a counter electrode, and the excitation unit is used to control the potential between the reference electrode and the working electrode to be kept relatively constant, thereby driving the electrochemiluminescence reaction at the surface of the working electrode. The traditional three-electrode electrochemiluminescence detection system has good performance in a single reaction tank, but has a plurality of problems in a double reaction tank. Currently, there is a three-electrode electrochemical luminescence sensor based on a shared working electrode, a reference electrode and a counter electrode, wherein a detection area and a quality control area (i.e. two reaction tanks) are integrated on the surface of a chip by adopting a parallel structure. In the electrochemical excitation process, the two reaction tanks share a negative feedback control loop formed by a working electrode and a reference electrode, and the current provided by the excitation unit for the double reaction tanks needs to flow through the same working electrode. The excitation mode enables the current distribution of the two reaction tanks to be limited by the shared working electrode, independent current regulation and control cannot be realized, and the current coupling effect can cause mutual interference of electrochemical processes in the two reaction tanks, so that the stability and controllability of a detection system are affected. There is a portable electrochemical excitation device at present, which adopts a potentiostat architecture of a traditional three-electrode system of a working electrode, a reference electrode and a counter electrode. When the device is used to excite electrochemiluminescence of a double reaction cell, the two reaction cells in the detection unit must share a potentiostat. The common framework can not realize the independent control of the potential between the reference electrode and the working electrode of each reaction tank, so that the electrode polarization effect is aggravated, and finally the detection sensitivity is reduced. Disclosure of Invention The utility model aims to overcome the defects and shortcomings of the prior art, and provides a five-way three-electrode electrochemiluminescence detector, which is provided with a negative feedback loop formed by two completely independent reference electrodes and a working electrode under the condition of sharing a counter electrode, wherein the electric potential between the reference electrodes and the working electrode forming the loop can be independently controlled, the currents of two reaction tanks can be independently regulated, and the two reaction tanks can independently perform electrochemiluminescence reaction. In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: The five-way three-electrode electrochemiluminescence detector comprises an electrochemiluminescence chip and a constant potential excitation device, wherein the electrochemiluminescence chip comprises an electrode plate, a sample adding plate, an upper cover and a lower cover, the electrode plate and the sample adding plate are arranged between the upper cover and the lower cover, a shared counter electrode, a first working electrode, a first reference electrode, a second working electrode and a second reference electrode are arranged on the front surface of the electrode plate, a driving electrode of the shared counter electrode is arranged on the back surface of the electrode plate, the shared counter electrode is connected with the driving electrode, and the sample adding plate covers the shared counter electrode, the first working electrode, the first reference electrode, the second working electrode and the second reference electrode; the bottom ends of the first working electrode, the first reference electrode, the second working electrode and the second reference electrode and the bottom end of the driving electrode are used as electric contact areas, a first reaction tank is formed by sharing the first side of the counter electrode, the first side of the sample adding sheet corresponding to the first working electrode and the first refer