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CN-224232036-U - Annular fluorescence excitation loop for transduction and synergy

CN224232036UCN 224232036 UCN224232036 UCN 224232036UCN-224232036-U

Abstract

The utility model discloses a ring-shaped fluorescence excitation loop for transduction and synergy, which comprises a slide, a dichroic mirror, a first reflecting mirror, a second reflecting mirror and a third reflecting mirror which are sequentially arranged to form a closed loop light path, wherein the dichroic mirror is arranged on the laser and fluorescence signal outgoing side of the slide, the dichroic mirror is used for reflecting incident laser to the first reflecting mirror and transmitting fluorescence signals from the slide, the first reflecting mirror is used for receiving residual laser reflected by the dichroic mirror and reflecting the residual laser to the second reflecting mirror, the second reflecting mirror is used for receiving the residual laser reflected by the first reflecting mirror and reflecting the residual laser reflected by the second reflecting mirror to the third reflecting mirror, and after the scheme is adopted, the laser utilization rate can be improved, so that the problem of low laser utilization rate in the prior art is practically solved.

Inventors

  • WU XIAOFENG
  • ZHAN SHIPING
  • CHENG SHENGBIN

Assignees

  • 佛山大学

Dates

Publication Date
20260512
Application Date
20250512

Claims (8)

  1. 1. A ring-shaped fluorescence excitation loop for transduction and synergy is characterized in that, The device comprises a slide, a dichroic mirror, a first reflecting mirror, a second reflecting mirror and a third reflecting mirror which are sequentially arranged to form a closed loop light path; The dichroic mirror is arranged on the laser and fluorescent signal outgoing side of the glass slide, and is used for reflecting incident laser to the first reflecting mirror and transmitting fluorescent signals from the glass slide; the first reflecting mirror is used for receiving the residual laser light reflected by the dichroic mirror and reflecting the residual laser light to the second reflecting mirror; The second reflector is used for receiving the residual laser reflected by the first reflector and reflecting the residual laser to the third reflector; The third reflector is used for receiving the residual laser reflected by the second reflector and reflecting the residual laser to the glass slide.
  2. 2. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The glass slide is made of quartz or optical glass, and a fluorescent substance layer is coated on the surface of the glass slide.
  3. 3. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The mirror surface of the dichroic mirror forms an angle of 45 degrees with the incident direction of the laser.
  4. 4. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The wave band of the dichroic mirror covers the laser wave band and the fluorescence signal wave band; The laser wave band is 900-1100nm; The fluorescence signal wave band is 400-800nm.
  5. 5. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The transmittance of the dichroic mirror is more than or equal to 90% of the fluorescence wavelength, and the reflectance of the dichroic mirror is more than or equal to 90% of the laser wavelength.
  6. 6. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The mirror surface of the first reflecting mirror forms an angle of 135 degrees with the incidence direction of the laser.
  7. 7. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The mirror surface of the second reflecting mirror forms an angle of 45 degrees with the incidence direction of the laser.
  8. 8. The loop fluorescence excitation circuit of claim 1, wherein the fluorescent excitation light is a fluorescent light, The mirror surface of the third reflecting mirror forms an angle of 135 degrees with the incidence direction of the laser.

Description

Annular fluorescence excitation loop for transduction and synergy Technical Field The utility model relates to the technical field of fluorescence excitation loops, in particular to a ring-shaped fluorescence excitation loop for transduction and synergy. Background The traditional fluorescence excitation system mostly adopts a single light path design, the laser utilization rate is low, incident laser only penetrates through a sample glass for a single time, residual laser without excited fluorescence is directly lost, resource waste is caused, in addition, the signal separation is dependent on a plurality of groups of band-pass filters, the laser and fluorescence signals are distinguished, light energy attenuation is caused, meanwhile, the system complexity and maintenance cost are increased, in addition, the background noise is obvious, the residual laser stray light of the filters is easy to form background interference, and the detection sensitivity is influenced. Disclosure of utility model The utility model aims to provide a ring-shaped fluorescence excitation loop for transduction and synergy, so as to solve the problem of low laser utilization rate in the prior art. In order to solve the technical problems, the utility model provides a ring-shaped fluorescence excitation loop for transduction and synergy, which comprises a slide, a dichroic mirror, a first reflecting mirror, a second reflecting mirror and a third reflecting mirror which are sequentially arranged to form a closed loop light path, wherein the dichroic mirror is arranged on the laser and fluorescence signal outgoing side of the slide, the dichroic mirror is used for reflecting incident laser to the first reflecting mirror and transmitting fluorescence signals from the slide, the first reflecting mirror is used for receiving residual laser reflected by the dichroic mirror and reflecting the residual laser to the second reflecting mirror, the second reflecting mirror is used for receiving the residual laser reflected by the first reflecting mirror and reflecting the residual laser reflected by the first reflecting mirror to the third reflecting mirror, and the third reflecting mirror is used for receiving the residual laser reflected by the second reflecting mirror and reflecting the residual laser reflected by the third reflecting mirror to the slide. In one embodiment, the glass slide is made of quartz or optical glass, and the surface of the glass slide is coated with a fluorescent substance layer. In one embodiment, the mirror surface of the dichroic mirror makes an angle of 45 ° with the laser light incident direction. In one embodiment, the wavelength band of the dichroic mirror covers the laser wavelength band (900-1100 nm) and the fluorescence signal wavelength band (400-800 nm). In one embodiment, the transmittance of the dichroic mirror is 90% or more for fluorescence wavelength and the reflectance of the dichroic mirror is 90% or more for laser wavelength. In one embodiment, the mirror surface of the first reflecting mirror forms an angle of 135 degrees with the incident direction of the laser. In one embodiment, the mirror surface of the second reflecting mirror forms an angle of 45 degrees with the incident direction of the laser. In one embodiment, the mirror surface of the third mirror is at an angle of 135 ° to the laser light incident direction. The beneficial effects of the utility model are as follows: 1. the laser utilization rate is obviously improved, namely, residual laser is recovered through a closed loop light path to carry out secondary excitation; 2. The hardware cost and complexity are obviously reduced, namely, a plurality of groups of band-pass filters are omitted, the light energy loss is reduced, and the system components are reduced; 3. Optimizing the signal-to-noise ratio of the fluorescent signal, namely improving the intensity of the fluorescent signal, reducing background noise and improving the detection sensitivity; 4. the compatibility and stability are enhanced, the modularized design is adapted to equipment such as a fluorescence microscope, a flow cytometer and the like, the light path calibration tolerance is improved, and the maintenance difficulty is reduced. Drawings In order to more clearly illustrate the technical solutions of the present utility model, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art. Fig. 1 is a schematic structural diagram provided in an embodiment of the present utility model. The reference numerals are as follows: 1. Slide, 2, dichroic mirror, 3, first reflecting mirror, 4, second reflecting mirror, 5, third reflecting mirror. Detailed Description The technical solutions in the embodiments of t