DE-102024132751-A1 - METHOD FOR ACTIVATING MITOCHONDRIA

Abstract

The present invention relates to a method for activating the mitochondrion, wherein mainly negative charges and reactive oxygen species are supplied to the mitochondrion from the outside via a negative charge and reactive oxygen species delivery unit to serve as material for the mitochondrial redox reaction. A calcium ion channel stimulation unit stimulates an in vivo calcium ion to transport the protein across the calcium on the inner mitochondrial membrane into a mitochondrial matrix space, such that the influx of calcium ions promotes mitochondrial depolarization and increases the activity of mitochondrial oxidative phosphorylation. This enhances mitochondrial activation and ATP production.

Inventors

• Chung-Tai Chang
• Chia-Hao Chang
• Ting-Yi Chang

Assignees

• Chia-Hao Chang
• Chung-Tai Chang
• Ting-Yi Chang

Dates

Publication Date

20260513

Application Date

20241110

Claims (3)

1. A method for activating the mitochondrion, comprising step 1, in which a negative charge and reactive oxygen are supplied to a mitochondrion, wherein the negative charge and reactive oxygen are supplied to the mitochondrion from outside via a negative charge and reactive oxygen supply unit to serve as material for the mitochondrial redox reaction; and, step 2, in which a calcium ion is supplied to the mitochondrion, wherein a calcium ion channel stimulation unit stimulates an in vivo calcium ion to transport a protein across a calcium ion on an inner membrane of the mitochondrion into a matrix space of the mitochondrion, whereby an influx of the calcium ion promotes depolarization of the mitochondrion and increases the activity of mitochondrial oxidative phosphorylation.
2. The method for activating the mitochondrion according to Claim 1 , wherein the unit for providing negative charges and reactive oxygen is a low-temperature plasma generator.
3. The method for activating the mitochondrion according to Claim 1 , wherein the stimulation unit of the calcium ion channel is a far-infrared generator with a power output exceeding 500W.

Description

BACKGROUND OF THE INVENTION The present invention relates to a method for activating mitochondria, and in particular for supplying the mitochondrion with negative charges (-e) and reactive oxygen (O) ex vivo by a unit for supplying negative charges and reactive oxygen. In this process, calcium ions are stimulated in vivo to pass through the calcium on the mitochondrial endomembrane in order to transfer proteins into the mitochondrial matrix via a calcium ion channel stimulation unit, thereby promoting mitochondrial activation and the formation of adenosine triphosphate (ATP). DESCRIPTION OF THE STATE OF THE ART The mitochondrion is a unit responsible for energy production within the cell, essentially the cell's "energy factory." Each mitochondrion consists of an outer membrane layer and an inner membrane layer, and the space enclosed by the inner membrane is called the matrix. The outer membrane of a mitochondrion is equipped with enzymes involved in biological oxidation, providing materials necessary for reactions within the mitochondrion. Within the mitochondrion, the matrix contains enzymes capable of metabolizing and converting carbohydrates, proteins, and fats into H₂O and CO₂ via the citric acid cycle. During this process, electrons are transferred through the electron transport chain and synthesized into adenosine triphosphate (ATP) through oxidative phosphorylation. ATP is the primary energy source for energy-consuming reactions in plants and animals. In biology, ATP is a type of nucleotide that serves as an energy currency for intracellular energy transfer, storing and transmitting chemical energy. ATP plays an important role in nucleic acid synthesis. Furthermore, ATP is the disodium guanylate in RNA and acts as a substitute during DNA transcription. However, energy production in the mitochondria requires a continuous redox reaction. The activation power of the redox reaction depends on the negative charge (-e) and the reactive oxygen species (O), which are the materials the mitochondria need to function. When these materials can be supplied to the mitochondria, the mitochondria become more active. Furthermore, the influx of calcium ions plays a crucial role in mitochondrial activation and ATP production, as follows: 1. Control of calcium ions within the mitochondrion: Calcium ions can enter the mitochondrion and influence its function. When the calcium level in the cytoplasm rises, calcium ions transport proteins across the calcium on the inner mitochondrial membrane into the mitochondrial matrix. The influx of calcium ions can promote mitochondrial depolarization and increase the activation of mitochondrial oxidative phosphorylation. 2. Promotion of oxidative phosphorylation: The increase in calcium ions activates various enzyme systems in the mitochondria, such as the key enzyme in the tricarboxylic acid cycle (TCA). The activation of these enzymes allows the mitochondria to accelerate the oxidation process of the substrate (also called zymolyte or functional substance) and increase the efficiency of the electron transport chain, ultimately contributing to the formation of ATP. 3. Increased ATP Synthesis: When calcium levels in the mitochondria rise, the tricarboxylic acid cycle (TCA cycle) and oxidative phosphorylation are accelerated. Oxidative phosphorylation is a type of cellular metabolic process that takes place in the inner mitochondrial membrane of eukaryotes or in the cell membrane of prokaryotes. The enzymes and energy released during the oxidation of various nutrients are used for ATP synthesis, directly increasing ATP production. ATP production is driven by the electron transport chain, and the processes promoted by calcium ions ensure the continuity and efficiency of ATP production. 4. Maintaining the cell's energy balance: Increased ATP production contributes to maintaining the cell's energy balance, especially under various stresses. This is also beneficial for cell repair and the restoration of normal function, thus preventing cell death due to energy deficiency. Therefore, the continuous supply of negative charges (-e) and reactive oxygen (O), which are required for the mitochondrial redox reaction from the outside, as well as the promotion of calcium ions in vivo, which transport proteins via the calcium on the inner membrane of the mitochondrion into the mitochondrial matrix space, ren, the activation of the mitochondrion and ATP production is increased. SUMMARY OF THE INVENTION A primary objective of the present invention is to provide a method for activating the mitochondria that increases mitochondrial activation and ATP production. The main feature of the present invention relates to step 1, in which negative charges and reactive oxygen are supplied from the outside to the mitochondrion via the units for supplying negative charges and reactive oxygen to serve as material for the mitochondrial redox reaction, and step 2, in which the stimulation unit of the calcium ion cha