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DE-112024002550-T5 - FILTER, CELL MEMBRANCE DESTRUCTION DEVICE AND CELL MEMBRANCE DESTRUCTION METHOD

DE112024002550T5DE 112024002550 T5DE112024002550 T5DE 112024002550T5DE-112024002550-T5

Abstract

A filter according to the present disclosure is a filter for disrupting cell membranes. The filter comprises a filter body having a first main surface with a projection and a second main surface on the side opposite the first main surface, and defining a plurality of through-holes through which the first main surface communicates with the second main surface. The projection is located between adjacent through-holes and extends from the filter body in a first direction. In the cross-section cut in a second direction, where the through-holes are adjacent to each other, the projection is arranged within an end section of the filter body located between the adjacent through-holes. The thickness of the projection in the second direction decreases towards the upper section of the projection.

Inventors

  • TAKAO MASAKAZU
  • KONDO TAKASHI
  • TOKITOU NATSUKO

Assignees

  • MURATA MANUFACTURING CO

Dates

Publication Date
20260507
Application Date
20240903
Priority Date
20230907

Claims (19)

  1. Filter for destroying cell membranes, the filter comprising: a filter body having a first main surface with a projection and a second main surface on a side opposite the first main surface, and a plurality of passages The passage openings are defined through which the first main surface is connected to the second main surface, wherein the projection is arranged between adjacent passage openings and, in a view from the side of the first main surface, projects away from the filter body in a first direction, in a cross-section cut in a second direction in which the passage openings are adjacent to each other, the projection is arranged within an end section of the filter body which lies between the adjacent passage openings, and the thickness of the projection in the second direction decreases towards an upper section of the projection.
  2. Filter by Claim 1 , wherein in the second direction the outer surface of the projection is curved from a central area of the filter body located between the adjacent passage openings towards the end section.
  3. Filter by Claim 2 , wherein the projection has at least in part of the outer surface of the projection a flat inclined surface which is inclined in the direction of the through-openings.
  4. Filter according to one of the Claims 1 until 3 , wherein the filter body comprises: a first filter body arranged between adjacent through-holes; and a second filter body, which is different from the first filter body and is arranged between adjacent through-holes, wherein the projection comprises: a first projection extending from the first principal face of the first filter body; and a second projection extending from the first principal face of the second filter body, and where a height of the projection is defined as the distance from the second principal face of the filter body to the upper portion of the projection, the height of the first projection differs from the height of the second projection.
  5. Filter according to one of the Claims 1 until 4 , wherein, in the view from the side of the first main surface of the filter body, the projection surrounds one or more through-openings.
  6. Filter according to one of the Claims 1 until 5 , where if the height of the projection is defined as the distance from the second main surface of the filter body to the upper section of the projection, the height of the projection changes continuously.
  7. Filter by Claim 5 , wherein, in a view from the side of the first main surface of the filter body, the area of a region surrounded by the upper section of the projection is larger than an opening area of the one or more through-holes.
  8. Filter according to one of the Claims 1 until 7 , with the protrusion having a pointed upper section.
  9. Filter by Claim 8 , wherein if a region is formed by drawing three imaginary straight lines, each abutting the outer perimeters of two adjacent passages under three adjacent passages, such that the three passages are contained within the region in the view from the side of the first principal face of the filter body, the pointed upper section is located within this region.
  10. Filter by Claim 8 , where if a height of the projection is defined as the distance from the second principal surface of the filter body to the upper section of the projection, a height of the projection at the pointed upper section is greater than a height of the projection at a different type of upper section.
  11. Filter by Claim 2 , wherein the projection has a lateral projection that extends from the outer surface.
  12. Cell membrane destruction device, comprising the filter according to one of the Claims 1 until 11 .
  13. Cell membrane destruction device after Claim 12 , further comprising a suction device which draws cells with cell membranes on the side of the first main surface of the filter body through the plurality of openings to one side of the second main surface.
  14. Cell membrane destruction device after Claim 13 , further comprising a container to which the filter is attached, wherein gas in the container is drawn in by the suction device at a position downstream of the filter.
  15. Cell membrane destruction device according to one of the Claims 12 until 14 , furthermore comprising a vibration device that vibrates the filter.
  16. Cell membrane destruction techniques, comprehensive: a step of providing a filter with a filter body having a first main surface with a projection and a second main surface on a side opposite the first main surface and defining a plurality of openings through which the first main surface communicates with the second main surface; a step of introducing cells with cell membranes onto the side of the first main surface of the filter body of the filter; and a step of destroying the cell membranes by the projection of the filter.
  17. Cell membrane destruction methods according to Claim 16 , furthermore, encompassing a step of recovering cell contents that have passed through the filter.
  18. Cell membrane destruction methods according to Claim 16 or 17 , wherein the step of destroying the cell membranes further comprises the suction of the cells on the side of the first main surface of the filter body through the plurality of openings onto one side of the second main surface.
  19. Cell membrane destruction methods according to Claim 18 , wherein the provisioning step comprises the provision of the filter attached to a container and the suction step comprises the suction of gas in the container at a position downstream of the filter.

Description

Technical field The present invention relates to a filter, a cell membrane destruction device and a cell membrane destruction method. State of the art For example, patent document 1 discloses a method for extracting intracellular substances, comprising a step in which cells are suspended in a solution containing benzalkonium chloride as a surfactant in a concentration in the range of 0.0005% to 0.01%, and a step in which a liquid with an osmotic pressure different from the intracellular osmotic pressure is added. Patent document 2 discloses a method in which, while a high-resistance electrical seal (gigaohm seal) is formed between a region of the cell membrane of a cell attached to a patch-clamp electrode and a tip opening of the electrode, the cell membrane is destroyed at the sealed section. The method described in patent document 1 comprises (a) a step in which a test cell is brought into contact with the tip opening of a patch-clamp pipette, and (b) a step in which a vacuum controlled by a pressure calibrator is applied to the cell using the patch-clamp pipette. List of objections Patent documents Patent document 1: Publication No. 2009-42Patent document 2: Publication No. 2004-294211 Brief description of the invention Technical problem Recently, there has been a need to efficiently destroy cell membranes. The present invention provides a filter, a cell membrane destruction device and a cell membrane destruction method, each of which is capable of efficiently destroying cell membranes. Solution to the problem A filter according to one aspect of the present invention is a filter for destroying cell membranes, wherein the filter comprises the following: a filter body comprising a first main surface with a projection and a second main surface on the side opposite the first main surface, and defining a plurality of openings through which the first main surface is connected to the second main surface, wherein the projection is arranged between adjacent through-openings and protrudes in a first direction away from the filter body, In a cross-section cut in a second direction where the through-holes are adjacent to each other, the projection is arranged within an end section of the filter body that lies between the adjacent through-holes, and The thickness of the projection decreases in the second direction towards an upper section of the projection. A cell membrane destruction device according to one aspect of the present invention comprises the filter according to the above aspect. A cell membrane destruction method according to one aspect of the present invention comprises: a step in which a filter is provided comprising a filter body having a first principal surface with a projection and a second principal surface on the opposite side of the first principal surface, and defining a plurality of through-holes through which the first principal surface communicates with the second principal surface; and a step in which cells containing cell membranes are fed to the side of the first main surface of the filter body of the filter. Advantages of the invention According to the present invention, a filter, a cell membrane destruction device and a cell membrane destruction method can be provided, each of which is capable of efficiently destroying cell membranes. Description of the drawings 1 Figure 1 schematically shows in top view an example of a filter 1 according to a first embodiment of the present invention, seen from the side of the first main surface.2 is an enlarged top view of part of a filter component.3 This is an enlarged perspective view of part of the filter section.4 This is a schematic cross-section of part of the filter section.5A is a schematic representation that illustrates an example of a step in the production of a filter.5B is a schematic representation that illustrates an example of one step in the production of the filter.5C is a schematic representation that illustrates an example of one step in the production of the filter.5D is a schematic representation that illustrates an example of one step in the production of the filter.5E is a schematic representation that illustrates an example of one step in the production of the filter.5F is a schematic representation that illustrates an example of one step in the production of the filter.5G is a schematic representation that illustrates an example of one step in the production of the filter.6 Figure 1 is a schematic view of an example of a cell membrane destruction device 2 according to the first embodiment of the present invention.7 is a flowchart of an example of a cell membrane destruction method according to the first embodiment of the present invention.8A Figure 1 is a schematic representation illustrating an example of a step in the cell membrane destruction process according to the first embodiment of the present invention.8B Figure 1 is a schematic representation illustrating an example of a step in the cell membrane destruction process