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EP-3521414-B1 - XYLARIA GRAMMICA

EP3521414B1EP 3521414 B1EP3521414 B1EP 3521414B1EP-3521414-B1

Inventors

  • KIM, JIN-CHEOL
  • KIM, TAE YOON
  • JANG, JA YEONG
  • YU, Nan Hee
  • CHI, WON-JAE
  • BAE, CHANG HWAN
  • YEO, JOO HONG

Dates

Publication Date
20260506
Application Date
20170927

Claims (5)

  1. Xylaria grammica EL 000614 strain having nematicidal activity against root knot nematode, wherein the Xylaria grammica EL 000614 strain has been deposited under the Budapest Treaty at the Korean Research Institute of Bioscience and Biotechnology and having the accession number of KCTC 13121BP.
  2. The Xylaria grammica EL 000614 strain according to Claim 1, wherein the root knot nematode belongs to Meloidogyne sp.
  3. The Xylaria grammica EL 000614 strain according to Claim 2, wherein the root knot nematode is Meloidogyne incognita or Meloidogyne hapla.
  4. A nematicidal microorganism formulation for root knot nematode containing, as an effective ingredient, the strain of any one of Claims 1 to 3, a spore, or a fungal hyphal mass thereof.
  5. A method for controlling root knot nematode comprising treating a crop, a crop seed, or a field for cultivation with the nematicidal microorganism formulation for root know nematodes of Claim 4.

Description

TECHNICAL FIELD The present invention relates to Xylaria grammica EL 000614 strain having nematicidal activity against root knot nematode and uses thereof. BACKGROUND ART Plant parasitic nematodes feeding on nutrients after their invasion of plant root are known as a pathogen that attacks a crop. Production loss of representative 40 crops caused by nematode infection makes up 10% of the loss of the entire production amount of crops, and the damage cost tends to increase every year. As the plant parasitic nematodes causing a damage on crops all over the world, there is a root knot nematode as a representative example. The root knot nematode (Meloidogyne spp.) has a broad host range like infecting about 2,000 kinds of plants, and, while parasitizing inside a root of a plant, it completes its life cycle by feeding on nutrients from the plant. In accordance with an increase in cultivation in facilities, number of the root knot nematodes has also increased dramatically, thus causing rapid increase in damage to crops. As four kinds of the nematodes that are taken seriously in agriculture, there are sweet potato root knot nematode (Meloidogyne incognita), carrot root knot nematode (Meloidogyne hapla), Java root knot nematode (Meloidogyne javanica), and peanut root knot nematode (Meloidogyne arenaria). Among them, Meloidogyne incognita is particularly problematic in cultivation facilities in South Korea. Meanwhile, as a conventional method for reducing or controlling a damage to crops caused by nematode infection, there are soil fuming, soil over-humidification, artificial increase of temperature inside a green house, crop rotation, or the like. However, as those methods exhibit an influence on crops and cannot control completely the nematodes, it cannot be said that they are a favorable method. Other than those, a method of using chemical pesticide has been developed, but it also has a problem like toxicity for human body and environment. Accordingly, to replace those methods, various studies are currently carried out. As a representative example of the study for controlling nematodes, there are studies as follows: 1) study of nematode control by using plant-derived resistant gene, 2) study using proteins and peptides which exhibit toxicity for nematodes, 3) study of nematode control by using a natural product or a plant metabolite, 4) study using nematode-repelling material and plant, 5) environment-friendly control method, and 6) study of nematode control using genetic information and protein information of nematodes. Accordingly, studies of selecting the strains with nematicidal activity by nematicidal activity screening for culture of various microorganisms and developing strains for controlling diseases that are caused by plant parasitic nematodes are continuously under progress. Meanwhile, in Korean Patent Registration No. 0574348, "Xylaria sp. AH001 strain producing griseofulvin, formulation for controlling plant diseases containing same, and method for controlling plant diseases by using same" is disclosed, and in Korean Patent Application Publication No. 2010-0116562, "Bacillus velezeneis G341 strain and method for controlling plant disease using same" is disclosed. Further, nematicidal activity of certain fungi have been described e.g. in FR 2 747 016 A1 and Anke et al: "Secondary metabolites with nemiticidal and antimicrobial activity from nematophagous fungi and ascomycetes", Canadian Journal of Botany, 1995, vol. 73, p. S932-S939.Fungi of the Xylaraicea family have been described previously as expressing compounds with antimicrobial activity or economic potential, see e.g. Schneider etal: "Xylarin, an antifungal Xylaria metabolite with an unusual tricyclic uronic acid moiety", Natural Product Letters, 1995, vol. 7, p. 309-316; and Gutierrez et al: "Compounds derived from endophytes: A review of phytochemistry and pharmacology", 2012, Current Medicinal Chemistry, p. 2992-3030. However, nothing has been described with regard to Xylaria grammica having nematicidal activity against root knot nematode as described in the present invention. DETAILED DESCRIPTION OF THE INVENTION TECHNICAL PROBLEMS TO BE SOLVED The present invention is devised under the circumstances described above, and, according to the present invention, strains having nematicidal activity against root knot nematode were screened by using various microorganisms to develop an environment-friendly method for controlling plant parasitic nematodes. As a result, it was found that the culture filtrate of Xylaria grammica exhibits a very potent nematicidal activity. Furthermore, among the four strains of Xylaria grammica, the nematicidal activity of EL 000614 strain was the most excellent, in particular. Thus, after examining the nematicidal activity against sweet potato root knot nematode (Meloidogyne incognita) and carrot root knot nematode (Meloidogyne hapla) as a main cause of root knot nematode disease by using, as a subject, various microor