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EP-3607038-B1 - CLEANING COMPOSITIONS AND USES THEREOF

EP3607038B1EP 3607038 B1EP3607038 B1EP 3607038B1EP-3607038-B1

Inventors

  • GORI, Klaus

Dates

Publication Date
20260506
Application Date
20180316

Claims (8)

  1. A cleaning composition comprising a DNase, a protease and at least one cleaning component, wherein the DNase has at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or 100% sequence identity to the amino acid sequence shown in SEQ ID NO 13, wherein the protease has at least 80% sequence identity to the amino acid sequence shown in SEQ ID NO 79, wherein the amount of DNase in the composition is from 0.01 to 1000 ppm and the amount of protease is from 0.01 to 1000 ppm, and wherein the composition comprising the DNase and the protease provides synergistic reduction or removal of biofilm or components thereof.
  2. The cleaning composition according to claim 1, wherein the protease has at least 85%, at least 90%, at least 95%, at least 98% or 100% sequence identity to the amino acid sequence shown in SEQ ID NO 79.
  3. The cleaning composition according to claim 1 or 2, wherein the amount of DNase in the composition is from 1 ppm to 1000 ppm, from 10 ppm to 1000 ppm, from 50 ppm to 1000 ppm, from 100 ppm to 1000 ppm, from 150 ppm to 1000 ppm, from 200 ppm to 1000 ppm, from 250 ppm to 1000 ppm, from 250 ppm to 750 ppm or from 250 ppm to 500 ppm; and the amount of protease is from 1 ppm to 1000 ppm, from 10 ppm to 1000 ppm, from 50 ppm to 1000 ppm, from 100 ppm to 1000 ppm, from 150 ppm to 1000 ppm, from 200 ppm to 1000 ppm, from 250 ppm to 1000 ppm, from 250 ppm to 750 ppm, from 250 ppm to 500 ppm.
  4. The cleaning composition according to any of the preceding claims, wherein the cleaning component is selected from surfactants, preferably anionic and/or nonionic, builders and bleach components.
  5. Use of a cleaning composition according to any of claims 1 to 4 for deep cleaning of an item, wherein the item is a textile or a surface.
  6. A method of formulating a cleaning composition according to any of claims 1 to 4, comprising adding a DNase, a protease and at least one cleaning component, wherein the DNase has at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or 100% sequence identity to the amino acid sequence shown in SEQ ID NO 13, wherein the protease has at least 80% sequence identity to the amino acid sequence shown in SEQ ID NO 79, and wherein the composition comprising the DNase and the protease provides synergistic reduction or removal of biofilm or components thereof.
  7. A kit intended for deep cleaning, wherein the kit comprises a solution of an enzyme mixture comprising a DNase and a protease, wherein the DNase has at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or 100% sequence identity to the amino acid sequence shown in SEQ ID NO 13, wherein the protease has at least 80% sequence identity to the amino acid sequence shown in SEQ ID NO 79, and wherein the composition comprising the DNase and the protease provides synergistic reduction or removal of biofilm or components thereof.
  8. A method of deep cleaning of an item, comprising the steps of: a) contacting the item with a cleaning composition according to any of claims 1 to 4; and b) optionally rinsing the item, wherein the item is preferably a textile.

Description

Reference to a Sequence Listing This application contains a Sequence Listing in computer readable form. Background of the Invention The present invention relates to compositions such as cleaning compositions comprising a mix of enzymes. The invention further relates to use of compositions comprising such enzymes in cleaning processes and/or for deep cleaning of organic stains, and methods for removal or reduction of components of organic matter. Description of the Related Art Enzymes have been used in detergents for decades. Usually a cocktail of various enzymes is added to detergent compositions. The enzyme cocktail often comprises various enzymes, wherein each enzyme targets its specific substrate e.g. amylases are active towards starch stains, proteases on protein stains and so forth. Textile surfaces and hard surfaces, such as dishes or the inner space of a laundry machine enduring a number of wash cycles, become soiled with many different types of soiling which may comprise proteins, grease, starch etc. One type of stains may be associated with organic matter such as biofilm, EPS, etc. Organic matter may comprise different molecules such as polysaccharides, extracellular DNA (eDNA), and proteins. Some organic matter comprises an extracellular polymeric matrix, which may be sticky or glueing, which when present on textile, attracts soils and may cause redeposition or backstaining of soil resulting in a greying of the textile. Additionally, organic matters such as biofilms often cause malodor issues as various malodor molecules can be adhered by the polysaccharides, extracellular DNA (eDNA), and proteins in the complex extracellular matrix and be slowly released to cause consumer noticeable malodor issues. WO 2015/155350 discloses detergent compositions comprising a DNase from a fungal source, where the compositions may comprise additional enzymes such as a protease. WO 2014/087011 discloses detergent compositions comprising a DNase from Bacillus subtilis or Bacillus licheniformis, where the compositions may comprise additional enzymes such as a protease. WO 2017/001471 discloses a method for laundering a textile comprising at least 20% polyester using a fungal or bacterial DNase, where additional enzymes such as a protease may be included. WO 2016/087619 discloses high pH detergent compositions comprising a protease variant engineered for improved stability at high pH. WO 2016/066756 and WO 2016/066757 each disclose protease variants comprising one or more substitutions at a large number of different positions. WO 2017/060518 is an intermediately published document disclosing detergent compositions comprising any of several DNases and any of a number of different proteases and protease variants. WO 2017/059802 are WO 2017/060475 are intermediately published documents disclosing detergent compositions comprising any of a number of different DNase sequences, where the compositions may comprise additional enzymes such as a protease. There is still a need for cleaning compositions which effectively prevent, reduce or remove stains e.g. associated with biofilm, such as protein and DNA. The present invention provides new compositions fulfilling such need. Summary of the Invention A first aspect of the present invention relates to a cleaning composition comprising a DNase, a protease and at least one cleaning component, wherein the DNase has at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or 100% sequence identity to the amino acid sequence shown in SEQ ID NO 13, wherein the protease has at least 80% sequence identity to the amino acid sequence shown in SEQ ID NO 79, wherein the amount of DNase in the composition is from 0.01 to 1000 ppm and the amount of protease is from 0.01 to 1000 ppm, and wherein the composition comprising the DNase and the protease provides synergistic reduction or removal of biofilm or components thereof. A cleaning composition of the invention may comprise at least 0.001 ppm DNase, at least 0.001 ppm protease and a cleaning component, wherein the cleaning component is selected from a. 0.1 to 15 wt% of at least one a surfactant;b. 0.5 to 20 wt% of at least one builder; andc. 0.01 to 10 wt% of at least one bleach component. The invention further relates to use of a cleaning composition comprising a DNase, a protease and at least one cleaning component for deep cleaning of an item, wherein the item is a textile or a surface. The invention further relates to a method of formulating a cleaning composition comprising adding a DNase, a protease and at least one cleaning component. The invention further relates to a kit intended for deep cleaning, wherein the kit comprises a solution of an enzyme mixture comprising a DNase and a protease. A method of deep cleaning of an item may comprise the steps of: a) contacting the item with a solution comprising an enzyme mixture comprising a DNase, a protease and a cleaning component, wherein the cleaning component is selected from 0