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EP-3634441-B1 - SKIN CARE APPLICATIONS OF EXTRACELLULAR METABOLITES FROM BACILLUS COAGULANS

EP3634441B1EP 3634441 B1EP3634441 B1EP 3634441B1EP-3634441-B1

Inventors

  • MAJEED, MUHAMMED
  • NAGABHUSHANAM, KALYANAM
  • ARUMUGAM, SIVAKUMAR
  • ALI, FURQAN
  • Majeed, Shaheen
  • MUNDKUR, Lakshmi

Dates

Publication Date
20260506
Application Date
20180604

Claims (7)

  1. A composition containing partially purified extracellular metabolite preparation from Bacillus coagulans for use in a method of skin protection by a) protecting the skin against UV-A and UV-B induced cell damage and apoptosis, b) reducing oxidative stress in the skin by scavenging ROS and c) reducing inflammation by decreasing the levels of inflammatory markers in the skin, wherein Bacillus coagulans is of strain Bacillus coagulans MTCC 5856.
  2. The composition as in claim 1 for the use according to claim 1, wherein effective concentration of the partially purified extracellular metabolite preparation is 0.01% v/v to 2.0% v/v of the total composition.
  3. The composition as in claim 1 for the use according to claim 1, wherein the composition is formulated with pharmaceutically/cosmeceutically acceptable excipients, adjuvants, bases, diluents, carriers, conditioning agents, bioavailability enhancers, antioxidants and preservatives and/or incorporated into formulations containing skin care ingredients and administered topically in the form of creams, gels, lotions, powder, serum, oil, suspensions, ointments, soaps, scrubs, emulsions, and compacts.
  4. A composition containing partially purified extracellular metabolite isolated from Bacillus coagulans for use as an anti-oxidant and anti-inflammatory agent, wherein Bacillus coagulans is of strain Bacillus coagulans MTCC 5856.
  5. The composition as in claim 4 for the use according to claim 4, wherein the composition is formulated with pharmaceutically/cosmeceutically acceptable excipients, adjuvants, bases, diluents, carriers, conditioning agents, bioavailability enhancers, and preservatives and/or incorporated into formulations containing skin care ingredients and administered topically in the form of creams, gels, lotions, powder, serum, oil, suspensions, ointments, soaps, scrubs, emulsions, and compacts.
  6. A composition containing partially purified extracellular metabolite preparation from Bacillus coagulans for use in a method of protecting skin fibroblasts from UV induced damage, said method comprising steps of bringing into contact skin fibroblast cells with effective concentration of the composition containing partially purified extracellular metabolite preparation from Bacillus coagulans to bring about the effect of protection against UV-A and UV-B induced cell damage and apoptosis, wherein Bacillus coagulans is of strain Bacillus coagulans MTCC 5856.
  7. The composition as in claim 6 for the use according to claim 6, wherein effective concentration of the partially purified extracellular metabolite preparation is 0.01% v/v to 2.0% v/v of the total composition.

Description

BACKGROUND OF THE INVENTION Field of the invention The invention in general relates to extracellular metabolite isolated from Bacillus coagulans MTCC 5856 for use in a method of skin protection. Description of prior art The human skin is exposed to a wide range of environmental pollutants and harmful UV radiation, damaging the skin and leading to premature aging. Exposure to these harmful pollutants and UV radiation will increase the production of reactive oxygen species (ROS) and inflammatory markers, increasing the oxidative stress and inflammation in the skin cells thereby damaging the cellular biomolecules (lipids, sugars, proteins, and polynucleotides). Protection against these agents will eventually improve the skin texture and lead to graceful aging. The skin cells inherently possess cellular defense systems against ROS and inflammatory agents, which include nonenzymatic molecules (glutathione, vitamins A, C, and E, and several antioxidants present in foods) and enzymatic scavengers of ROS, with superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX). Apart from these, many natural molecules confer protection against the UV and ROS induced damage which are tapped commercially by different players in the cosmetic industry. Probiotics are now garnering much attention in the skin care arena. Probiotic of the genera Lactobacillus and Bifidobacteria are reported for treatment of acne, rosacea, protection against aging, photo damage and UV: 1. Mary-Margaret Kober, Whitney P. Bowe, The effect of probiotics on immune regulation, acne, and photoaging, International Journal of Women's Dermatology, Volume 1, Issue 2, June 2015, Pages 85-892. Audrey Guéniche, David Philippe, Philippe Bastien, Stephanie Blum, Elif Buyukpamukcu, and Isabelle Castiel-Higounenc, Probiotics for photoprotection, Dermatoendocrinol. 2009 Sep-Oct; 1(5): 275-279 US 2015/0044317 A1 discloses a method to treat existing environmentally damaged skin in a subject comprising topically applying to affected skin a composition comprising an anti-aging amount of an extracellular product of Bacillus coagulans, in particular Bacillus coagulans hammer strain Accession No. ATCC 31284, and a dermatologically acceptable carrier. The skin may be a skin damaged by sun, wind, extreme temperature, or a combination thereof, prior to the topical application of the composition. WO 2015/100405 A1, which corresponds to EP 2 989 197 A0, discloses a method for producing partially purified extracellular metabolite preparation from the probiotic bacterial strain Bacillus coagulans SBC-37 (Deposited in the Microbial Type Culture Collection and Gene Bank as strain number MTCC 5856) exhibiting 99% genetic homology with the known bacterial strains Bacillus coagulans ATCC 31284, Bacillus coagulans NBRC 3887 and Bacillus coagulans ATCC 7050. Probiotics strains alone or in combination with food have shown antioxidant activity and reduce damages caused by oxidation ((Yang Wang, Yanping Wu, Yuanyuan Wang, Han Xu, Xiaoqiang Mei, Dongyou Yu, Yibing Wang and Weifen Li, Antioxidant Properties of Probiotic Bacteria, Nutrients. 2017 May; 9(5): 521). The extracelluar metabolities of probiotics are also reported to confer skin protection against microbial infections (US7544363B2). These products are now increasingly being used in skin care applications (Bacillus Ferment, Ganeden Inc, https://cosmetics.specialchem.com/inci/bacillus-ferment, accessed 28 May 2018). However, it is well known in the scientific art that biological effects of probiotics or products thereof are strain specific and cannot be generalised among genera, species and strains (Probiotics: In Depth/NCCIH, U.S. Department of Health and Human Services, National Institutes of Health). Hence, there exists a need to find a superior probiotic strain and its extracellular product that can be used effectively as a skin protection agent. The present invention solves the above mentioned problem by disclosing the beneficial effects of partially purified extracellular metabolite preparation of Bacillus coagulans for skin protection/care. It is the principle objective of the invention to disclose a composition containing partially purified extracellular metabolite preparation of Bacillus coagulans MTCC 5856 for use in a method of skin protection by protection against UV radiation and UV induced DNA damage. It is another objective of the invention to disclose a composition containing partially purified extracellular metabolite preparation of Bacillus coagulans MTCC 5856 for use as an anti-oxidant and anti-inflammatory agent. The present invention fulfills the above mentioned objectives and provides further related advantages. DEPOSIT OF BIOLOGICAL MATERIAL The deposit of biological material Bacillus coagulans bearing accession number MTCC 5856, mentioned in the instant application has been made on 19th September 2013 at Microbial Type Culture Collection & Gene Bank (MTCC), CSIR-Institute of Microbial Technolo