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EP-3829559-B1 - INHIBITORS OF HISTONE DEACETYLASE USEFUL FOR THE TREATMENT OR PREVENTION OF HIV INFECTION

EP3829559B1EP 3829559 B1EP3829559 B1EP 3829559B1EP-3829559-B1

Inventors

  • LIU, JIAN
  • CLAUSEN, Dane James
  • YU, WENSHENG
  • KELLY, JOSEPH, M.
  • KIM, HYUNJIN, M.
  • KOZLOWSKI, JOSEPH, A.

Dates

Publication Date
20260513
Application Date
20190726

Claims (10)

  1. A compound of the formula: wherein is a five-membered heteroaryl ring which is optionally substituted with halo, cyano or C 1-3 alkyl; is selected from isoxazolyl, oxazolyl or thiazolyl which is optionally substituted with C 1-3 alkyl; R 1 is phenyl or heteroaryl, wherein said phenyl and heteroaryl groups are optionally substituted with one to three groups independently selected from the group consisting of halo, oxo, cyano, R 4 , R 6 , OR 4 , OR 6 and SO 2 R 4 ; R 2 is selected from the group consisting of NH 2 , NHR 4 , NHR 6 , and NHCH 2 R 6 ; R 3 is selected from hydrogen or C 1-6 alkyl; or R 2 and R 3 can be taken together with the atoms to which they are attached to form a 5, 6 or 7 membered heterocyclyl group which is optionally substituted with oxo; each R 4 is independently hydrogen or C 1-6 alkyl, which is optionally substituted with one to three halo; each R 5 is independently hydrogen or C 1-6 alkyl, which is optionally substituted with N(R 4 ) 2 or OR 4 ; R 6 is (a) heterocyclyl, (b) C 3-6 cycloalkyl, (c) phenyl, or (d) heteroaryl, which may be monocyclic or bicyclic, wherein said heterocyclyl, cycloalkyl, phenyl and heteroaryl groups are optionally substituted with one to two groups independently selected from the group of oxo, R 5 , OR 4 and heteroaryl; R a is hydrogen or halo; R b is hydrogen or halo; or a pharmaceutically acceptable salt thereof; wherein a heteroaryl is a monocyclic or bicyclic ring system of up to 10 atoms in each ring, wherein at least one ring is aromatic, and at least one ring contains from 1 to 4 heteroatoms selected from the group consisting of O, N and S; and wherein a heterocyclyl is a nonaromatic monocyclic or bicyclic ring system of up to 10 atoms in each ring containing from 1 to 4 heteroatoms selected from the group consisting of O, N, S, SO, or SO 2 .
  2. The compound of Claim 1 wherein is selected from imidazolyl, isoxazolyl, oxadiazolyl, oxazolyl, pyrazolyl or triazolyl, wherein said groups are optionally substituted with halo, cyano or C 1-3 alkyl; or a pharmaceutically acceptable salt thereof.
  3. The compound of any of Claims 1 to 2 wherein R 1 is dihydroisoquinolinyl, imidazolyl, isoquinolinyl, napthyridinyl, phenyl, pyrazinyl, pyridinyl, quinolinyl or quinoxalinyl, wherein said groups are optionally substituted with one to three groups optionally selected from the group consisting of halo, oxo, cyano, R 4 , R 6 , OR 4 , OR 6 and SO 2 R 4 ; or a pharmaceutically acceptable salt thereof.
  4. The compound of any of Claims 1 to 3 wherein R 2 is NH 2 , or a pharmaceutically acceptable salt thereof.
  5. A compound according to claim 1 selected from the group consisting of: ((S)-7-amino-7-(4-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-6-(2-(1-amino-7-(oxazol-2-yl)-7-oxoheptyl)-1H-imidazol-4-yl)-2-ethylisoquinolin-1(2H)-one; (S)-7-amino-1-(oxazol-2-yl)-7-(5-(quinolin-6-yl)-1H-imidazol-2-yl)heptan-1-one; (S)-7-amino-7-(4-(7-methoxyquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-amino-7-(4-(6-cyclopropyl-2-methoxypyridin-3-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-amino-7-(4-chloro-2-(4-fluorophenyl)-1H-imidazol-5-yl)-1-(oxazol-2-yl)heptan-1-one; 7-amino-7-(5-(2-methylquinolin-6-yl)oxazol-2-yl)-1-(oxazol-2-yl)heptan-1-one (35, L-006157881-001T) and (37, L-006157885-001C); (S)-7-amino-7-(5-(7-methoxy-2-methylquinolin-6-yl)oxazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-amino-7-(5-(2-methoxypyridin-3-yl)oxazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-amino-7-(1-methyl-4-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-amino-7-(1-ethyl-4-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-amino-1-(isoxazol-3-yl)-7-(4-(7-methoxy-2-methylquinolin-6-yl)-1H-imidazol-2-yl)heptan-1-one; (S)-6-(2-(1-amino-7-(isoxazol-3-yl)-7-oxoheptyl)-1H-imidazol-4-yl)-1-methylquinolin-2(1H)-one; (S)-7-amino-1-(isoxazol-3-yl)-7-(4-(2-methoxypyridin-3-yl)-1H-imidazol-2-yl)heptan-1-one; (S)-7-amino-1-(isoxazol-3-yl)-7-(4-(7-methoxyquinolin-6-yl)-1H-imidazol-2-yl)heptan-1-one; (S)-6-(2-(1-amino-7-(isoxazol-3-yl)-7-oxoheptyl)-1H-imidazol-5-yl)-7-methoxy-1-methylquinolin-2(1H)-one; (S)-7-amino-7-(5-(2-fluorophenyl)-1H-imidazol-2-yl)-1-(oxazol-4-yl)heptan-1-one; (S)-7-amino-7-(5-(2-fluorophenyl)-1H-imidazol-2-yl)-1-(isoxazol-3-yl)heptan-1-one; (R)-5-(5-(4-fluorophenyl)-1H-imidazol-2-yl)-5-(6-(oxazol-2-yl)-6-oxohexyl)pyrrolidin-2-one; 4-(4-(4-fluorophenyl)-1H-imidazol-2-yl)-4-(6-(oxazol-2-yl)-6-oxohexyl)oxazolidin-2-one; (S)-5-(4-(2-fluoro-4-(oxazol-2-yl)phenyl)-1H-imidazol-2-yl)-5-(6-(isoxazol-3-yl)-6-oxohexyl)pyrrolidin-2-one; (S)-5-(4-(4-cyclopropyl-2-fluorophenyl)-1H-imidazol-2-yl)-5-(6-(isoxazol-3-yl)-6-oxohexyl)pyrrolidin-2-one; (S)-5-(4-(2-fluorophenyl)-1H-imidazol-2-yl)-5-(6-(isoxazol-3-yl)-6-oxohexyl)pyrrolidin-2-one; (R)-5-(6-(isoxazol-3-yl)-6-oxohexyl)-5-(5-(7-methoxy-2-methylquinolin-6-yl)-1H-imidazol-2-yl)pyrrolidin-2-one; (S)-6-(6-(isoxazol-3-yl)-6-oxohexyl)-6-(4-(2-methoxypyridin-3-yl)-1H-imidazol-2-yl)piperidin-2-one; (S)-7-(4-(2-fluorophenyl)-1H-imidazol-2-yl)-7-(6-(isoxazol-3-yl)-6-oxohexyl)azepan-2-one; (S)-7-(ethylamino)-7-(5-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-(methylamino)-7-(5-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-methoxy-1-methyl-6-(2-(1-(methylamino)-7-(oxazol-2-yl)-7-oxoheptyl)-1H-imidazol-5-yl)quinolin-2(1H)-one; (S)-6-(2-(1-(ethylamino)-7-(oxazol-2-yl)-7-oxoheptyl)-1H-imidazol-5-yl)-7-methoxy-1-methylquinolin-2(1H)-one; (S)-7-((1-methylpiperidin-4-yl)amino)-7-(5-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-(5-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)-7-((tetrahydro-2H-pyran-4-yl)amino)heptan-1-one; (S)-7-(5-(7-methoxy-2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)-7-((tetrahydro-2H-pyran-4-yl)amino)heptan-1-one; (S)-7-(benzylamino)-7-(5-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-(benzylamino)-7-(5-(7-methoxy-2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (S)-7-(5-(2-methylquinolin-6-yl)-1H-imidazol-2-yl)-7-morpholino-1-(oxazol-2-yl)heptan-1-one; (S)-7-(5-(7-methoxy-2-methylquinolin-6-yl)-1H-imidazol-2-yl)-7-((3-methoxycyclobutyl)amino)-1-(oxazol-2-yl)heptan-1-one; (S)-7-(5-(7-methoxy-2-methylquinolin-6-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)-7-(pyrimidin-2-ylamino)heptan-1-one; (S)-7-amino-7-(5-(4-fluorophenyl)isoxazol-3-yl)-1-(isoxazol-3-yl)heptan-1-one; (R)-7-amino-7-(5-(4-fluorophenyl)isoxazol-3-yl)-1-(isoxazol-3-yl)heptan-1-one; (7S)-7-amino-7-[1-(4-fluorophenyl)-1H-pyrazol-3-yl]-1-isoxazol-3-ylheptan-1-one; 7-amino-7-[1-(4-fluorophenyl)-1H-pyrazol-4-yl]-1-isoxazol-3-ylheptan-1-one; 7-amino-7-[1-(4-fluorophenyl)-1H-pyrazol-4-yl]-1-isoxazol-3-ylheptan-1-one; 7-amino-7-[2-(4-fluorophenyl)-2H-1,2,3-triazol-4-yl]-1-isoxazol-3-ylheptan-1-one; 7-amino-7-[2-(4-fluorophenyl)-2H-1,2,3-triazol-4-yl]-1-isoxazol-3-ylheptan-1-one; (7S)-7-amino-7-[5-(2-fluorophenyl)isoxazol-3-yl]-1-isoxazol-3-ylheptan-1-one; (7S)-7-amino-1-isoxazol-3-yl-7-[5-(2-methyl-2H-indazol-5-yl)isoxazol-3-yl]heptan-1-one; (7R)-7-amino-7-[1-(2-methyl-2H-indazol-5-yl)-1H-pyrazol-3-yl]-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-[1-(2-methyl-2H-indazol-5-yl)-1H-pyrazol-3-yl]-1-(1,3-oxazol-2-yl)heptan-1-one; (S)-7-amino-7-(5-(5-fluoro-2-methoxypyridin-4-yl)-1H-imidazol-2-yl)-1-(oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[5-(cyclobutyloxy)-2-fluorophenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-[5-(5-cyclopropylpyrazin-2-yl)-1H-imidazol-2-yl]-1-(1,3-oxazol-2-yl)heptan-1-one; 5-{2-[(1S)-1-amino-7-(1,3-oxazol-2-yl)-7-oxoheptyl]-1H-imidazol-5-yl}pyrazine-2-carbonitrile; 6-{2-[(1S)-1-amino-7-(1,3-oxazol-2-yl)-7-oxoheptyl]-1H-imidazol-5-yl}pyrazine-2-carbonitrile; (7S)-7-amino-7-{5-[2-fluoro-4-(1H-pyrazol-1-yl)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-[5-(2,5-difluorophenyl)-1H-imidazol-2-yl]-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-[5-(2,3-difluorophenyl)-1H-imidazol-2-yl]-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[2-fluoro-5-(trifluoromethyl)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[2-fluoro-3-(trifluoromethyl)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[2-fluoro-4-(trifluoromethyl)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-[5-(2-fluoro-4-methoxyphenyl)-1H-imidazol-2-yl]-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[2-fluoro-4-(trifluoromethoxy)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[2-fluoro-5-(trifluoromethoxy)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-{5-[2-fluoro-4-(methylsulfonyl)phenyl]-1H-imidazol-2-yl}-1-(1,3-oxazol-2-yl)heptan-1-one; (7S)-7-amino-7-(5-{2-fluoro-3-methoxy-5-[(trans-3-methoxycyclobutyl)oxy]phenyl}-1H-imidazol-2-yl)-1-(1,3-oxazol-2-yl)heptan-1-one; (S)-5-(1-amino-7-(oxazol-2-yl)-7-oxoheptyl)-2-(4-fluorophenyl)-1H-imidazole-4-carbonitrile; (S)-5-(1-amino-7-(oxazol-2-yl)-7-oxoheptyl)-2-(4-fluorophenyl)oxazole-4-carbonitrile; (S)-7-amino-7-(4-chloro-5-(4-fluorophenyl)-1H-imidazol-2-yl)-1-(isoxazol-3-yl)heptan-1-one; or a pharmaceutically acceptable salt thereof.
  6. A pharmaceutical composition comprising a compound of any previous Claim, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
  7. A compound of any one of claims 1-5, or a pharmaceutically acceptable salt thereof, for use in the treatment of infection by HIV or for the treatment, prophylaxis, or delay in the onset or progression of AIDS.
  8. The pharmaceutical composition of claim 6, further comprising one or more additional therapeutic agents selected from raltegravir, lamivudine, abacavir, ritonavir, darunavir, atazanavir, emtricitabine, tenofovir, rilpivirine, doravirine, EFdA and lopinavir.
  9. A combination comprising a compound of any one of claims 1-5, or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from raltegravir, lamivudine, abacavir, ritonavir, darunavir, atazanavir, emtricitabine, tenofovir, rilpivirine, doravirine, EFdA and lopinavir, for use in treating infection by HIV or for treating, preventing or delaying the onset or progression of AIDS.
  10. A compound of any one of claims 1-5, or a pharmaceutically acceptable salt thereof, for use in therapy.

Description

FIELD OF THE INVENTION The present invention relates to inhibitors of histone deacetylase, compositions comprising at least one inhibitor of histone deacetylase, and methods of using the inhibitors of histone deacetylase for treating or preventing HIV infection in a subject. BACKGROUND OF THE INVENTION DNA in the nucleus of the cell exists as a hierarchy of compacted chromatin structures. The basic repeating unit in chromatin is the nucleosome, which consists of a histone octamer of proteins in the nucleus of the cell around which DNA is wrapped twice. The orderly packaging of DNA in the nucleus plays an important role in the functional aspects of gene regulation. Covalent modifications of the histones have a key role in altering chromatin higher order structure and function, and ultimately, gene expression. The covalent modification of histones, such as acetylation, occurs by enzymatically mediated process. Regulation of gene expression through the inhibition of the nuclear enzyme histone deacetylase (HDAC) is one of the several possible regulatory mechanisms whereby chromatin actively can be affected. The dynamic homeostasis of the nuclear acetylation of histone can be regulated by the opposing activity of the enzymes histone acetyl transferase (HAT) and histone deacetylase (HDAC). Transcriptionally silent chromatin can be characterized by nucleosomes with low levels of acetylated histones. Acetylation reduces the positive charge of histones, thereby expanding the structure of the nucleosome and facilitating the interaction of transcription factors with the DNA. Removal of the acetyl group restores the positive charge, condensing the structure of the nucleosome. While histone acetylation can activate DNA transcription, enhancing gene expression, histone deacetylase can reverse the process and can serve to repress gene expression. Inhibition of the histone deacetylase (HDAC inhibition) can also increase the activation of DNA transcription. See, for example, Grunstein, Nature, 389, 349-352 (1997); Pazin et al., Cell 89, 325-328 (1997); Wade et al., Trends Biochem Sci. 22, 128-132 (1997); and Wolffe, Science 272, 371-372 (1996). With the introduction of combination antiretroviral therapy (ART), HIV became a controllable chronic disease. The combination of ART (cART) targets specific stages of the viral life cycle, and is effective at combatting active viral load down to undetectable levels. However, HIV persists within the body of infected individuals undergoing therapy, and cessation of ART leads to a viral rebound within 3-4 weeks. The HIV can persist in resting memory and naive CD4+ T cells and other long-lived cells, such as infected astrocytes and cells of macrophage lineage. HIV can persist in these resting cells by establishing a latent or "silent" infection. In these cells, virus is integrated into the host genome, but viral production does not occur as a result of inhibition of both viral transcriptions from proteins. However, these latently infected cells still do contain replication competent virus, and once cART is stopped, rebound in plasma HIV RNA is observed in nearly all patients. One approach currently being explored to eliminate latently infected CD4+ T cells is to activate viral production from these cells in the presence of cART, when the production of the virus should kill the infected cells. Histone deacetylase inhibitors have shown promise in vitro in activating virus production from latent infected cells, and therefore this class of drugs is being studied as part of a strategy aimed at a cure of HIV. Eleven members of the HDAC family has been identified in humans, which share a conserved catalytic domain and are grouped into two classes: class I (1,2,3,8), homologous to yeast Rpd3; and class IIa (4,5,7,9) and IIb (6, 10), homologous to yeast Hdal. HDAC 11 shares homology with both classes, but is at the same time distinct from all the other ten subtypes. The first generation of HDAC inhibitors (HDACi) are promising therapeutic agents against cancer and other diseases, and showed in vitro activation of virus production from latent infected cells. However, due to their poor selectivity, those that entered clinical trials, all show similar adverse effects. The poorly selective HDACi's are not suitable for healthy HIV patents on cART, thus the interest is high for the discovery and development of novel and subtype selective HDAC inhibitors. WO2006/061638 discloses other HDAC inhibitors. SUMMARY OF THE INVENTION The references to methods of treatment in the subsequent paragraphs of this description are to be interpreted as references to the compounds, pharmaceutical compositions and medicaments of the present invention for use in a method for treatment of the human (or animal) body by therapy (or for diagnosis). In one aspect, the present invention provides Compounds of Formula I: or a pharmaceutically acceptable salt thereof as defined in the appended claims. The Compounds of Formula I a