Search

EP-3928633-B1 - PREPARATION METHOD OF BIO-FERMENTED LIQUID FEED, BIO-FERMENTED LIQUID FEED AND BIO-FERMENTED EMULSION LIQUID FEED

EP3928633B1EP 3928633 B1EP3928633 B1EP 3928633B1EP-3928633-B1

Inventors

  • WANG, XIAOMING
  • DENG, Dun
  • SHU, Jiancheng
  • TANG, Xiangshan
  • LUO, JIE

Dates

Publication Date
20260506
Application Date
20200929

Claims (8)

  1. A preparation method of bio-fermented liquid feed, including the following steps: 1) mixing corn, broken rice, barley and wheat in a weight ratio of 1: (0-5): (0-5): (0-5) to obtain a first substrate of enzymolysis; 2) mixing the first substrate of enzymolysis obtained in step 1) with water, adding α-amylase according to the ratio of 10-500U per gram of the first substrate of enzymolysis, and raising the temperature to 70-100 °C for liquefaction enzymatic hydrolysis for 30-120min to obtain a first enzymolysis slurry A; 3) adjusting the temperature of the first enzymolysis slurry A obtained in step 2) to 40-80 °C, adding β-amylase according to the ratio of 100-2000 U per gram of the first substrate of enzymolysis, adding pullulanase according to the ratio of 0.1-100 ASPU per gram of the first substrate of enzymolysis, adding glucoamylase according to the ratio of 0-1000 U per gram of the first substrate of enzymolysis, add xylanase according to the ratio of 15-1000 U per gram of the first substrate of enzymolysis, adding β-glucanase according to the ratio of 5-1000 U per gram of the first substrate of enzymolysis, and enzymolyzing for 0-72 h to obtain a second enzymolysis slurry A; 4) adjusting the temperature of the second enzymolysis slurry A obtained in step 3) to 25-40 °C, adding Lactobacillus according to the ratio of 1×10 5 -1×10 8 cfu per gram of the first substrate of enzymolysis, adding neutral protease according to the ratio of 1-1000 U per gram of the first substrate of enzymolysis, adding acid protease according to the ratio of 1-1000 U per gram of the first substrate of enzymolysis, and performing anaerobic fermentation for 10-72h to obtain a component A; 5) mixing and sterilizing the soybean meal and water, adjusting the temperature to 40-70 °C, adjusting the pH to 7-10, adding alkaline protease according to the ratio of 1-10000 U per gram of soybean meal, enzymolyzing for 0.5-6 h, then adjusting the pH to 6-8, adding neutral protease according to the ratio of 1-1000 U per gram of soybean meal, and enzymolyzing for 0.5-6 h to obtain a first enzymolysis slurry B; 6) adjusting the temperature of the first enzymolysis slurry B obtained in step 5) to 25-40 °C, adding Lactobacillus according to the ratio of 1×10 5 -1×10 8 cfu per gram of soybean meal, and adding acid protease according to the ratio of 1-1000 U per gram of soybean meal, performing anaerobic fermentation for 10-72 h to obtain a component B; 7) mixing the component A obtained in step 4) and the component B obtained in step 6) with a base material to obtain a bio-fermented liquid feed; the base material comprises the following components by mass percentage: 0-20% quick-breaking soybeans or puffed soybeans, 1-10% fish meal, 0-10% intestinal membrane protein powder, 1-10% yeast extract, 0-10% chocolate powder, 1-50% whey powder, 1-50% whole milk powder, 0-10% glucose, 1-20% sucrose, 0.1-5% calcium hydrogen phosphate, 0.1-5% calcium formate, 0.1-10% fat powder and 2-10% premix feed; wherein the step 1) and the step 5) are not limited in time sequence.
  2. The preparation method according to claim 1, wherein the corn, broken rice, barley and wheat in step 1) and the soybean meal in step 5) are crushed before being mixed with water.
  3. The preparation method according to claim 1 or 2, wherein the weight ratio for mixing the first substrate of enzymolysis in step 1) and water is 1: (1-10).
  4. The preparation method according to any of claims 1 to 3, wherein the weight ratio for mixing the soybean meal and water in step 5) is 1: (1-20).
  5. The preparation method according to any of claims 1 to 4, wherein in step 7), the weight ratio of component A, component B and base material is 100: (10-1000): (10-100).
  6. A bio-fermented liquid feed prepared by the preparation method of any of claims 1 to 5.
  7. A bio-fermented emulsion liquid feed based on the preparation method of any of claims 1 to 5 or the bio-fermented liquid feed of claim 6, wherein the preparation method of the bio-fermented emulsion liquid feed comprises the following steps: grinding, emulsifying and homogenizing the bio-fermented liquid feed to obtain the bio-fermented emulsion liquid feed.
  8. The bio-fermented emulsion liquid feed according to claim 6 or 7, wherein the particle size of the bio-fermented emulsion liquid feed is 0.01-1000 µm.

Description

CROSS REFERENCE TO RELATED APPLICATION This application claims the priority of Chinese Patent Application No. 202010590206.X, entitled "Preparation method of bio-fermented liquid feed, bio-fermented liquid feed and bio-fermented emulsion liquid feed" filed with China National Intellectual Property Administration on June 24, 2020. TECHNICAL FIELD The application relates to the technical field of feed preparation, in particular to a preparation method of bio-fermented liquid feed, bio-fermented liquid feed and bio-fermented emulsion liquid feed. BACKGROUND ART In order to improve the production efficiency of sows and the utilization rate of shed, most pig farms now adopt early weaning of piglets in the production technology. However, after early weaning, affected by changes in nutrition, physiology, psychology, environment and many other factors, piglets often suffer from weaning stress syndrome, such as no feeding or low feed intake, diarrhea, low immunity and susceptibility to illness. After weaning, the feed intake of piglets will inevitably decrease due to the sudden change of food from liquid breast milk to solid feed. The dry matter content of breast milk is only 20%, and these dry matter are composed of lactose, cream and lactoprotein, which are highly digestible. Most of the creep feed contain a lot of starch and plant protein, which are uneasy to digest. Newly weaned piglets have problems of insufficient gastric acid secretion, low digestive enzyme content and activity, incomplete immune system development and disordered intestinal microecosystem. Weaning stress problem can not be effectively solved. CN 108 041 310 A provides a method for preparing a liquid feed for weaned piglets with balanced nutrition, high digestibility, good palatability, and low addition of antibiotics and zinc oxide. However, the feed is added a small amount of antibiotics, and the liquid feed provided by this patent stratifies in a short time during storage, which is not conducive to commercial promotion. CN 111 296 659 A provides a fermented feed for weaned piglets; however, the fermented feed was prepared by solid state fermentation. Due to its significant difference in texture from breast milk, the feed intake of weaned piglets may decrease to a certain extent. CN 102 845 645 A discloses a method for preparing a soluble and digestible feed for young livestock and poultry, but it also includes drugs in the feed, and no animal experiments have been conducted to verify the effect of the feed. CN 105 746 918 A provides a nursing feed for weak piglets, but the patent only records the raw material components of the feed, and does not disclose the method of preparation and animal experiment effect. SUMMARY OF THE APPLICATION The purpose of the present application is achieved by the technical solution according to the claims. The preparation method of bio-fermented liquid feed, bio-fermented liquid feed and bio-fermented emulsion liquid feed provided in the present application may overcome the physiological problems of weaned piglets, such as insufficient gastric acid secretion, low digestive enzyme content and activity, incomplete immune system development and disordered intestinal microecosystem. The bio-fermented liquid feed provided by the present application has advantages of good palatability, high nutrition, easy digestion, low acidity and similarity to breast milk, the emulsion liquid feed provided by the present invention may ensure that no stratification will occur within 30 days, and only slight stratification will occur within 30 to 90 days. The bio-fermented liquid feed and the bio-fermented emulsion liquid feed may both effectively alleviate the weaning stress syndrome of piglets, and realize the perfect transition from breast milk to conventional solid feed. The application provides a preparation method of bio-fermented liquid feed, including the following steps: 1) mixing corn, broken rice, barley and wheat in a weight ratio of 1: (0-5): (0-5): (0-5) to obtain a first substrate of enzymolysis;2) mixing the first substrate of enzymolysis obtained in step 1) with water, adding α-amylase according to the ratio of 10-500 U per gram of the first substrate of enzymolysis, and raising the temperature to 70-100 °C for liquefaction enzymatic hydrolysis for 30-120min to obtain a first enzymolysis slurry A;3) adjusting the temperature of the first enzymolysis slurry A obtained in step 2) to 40-80 °C, adding β-amylase according to the ratio of 100-2000 U per gram of the first substrate of enzymolysis, adding pullulanase according to the ratio of 0.1-100 ASPU per gram of the first substrate of enzymolysis, adding glucoamylase according to the ratio of 0-1000 U per gram of the first substrate of enzymolysis, adding xylanase according to the ratio of 15-1000 U per gram of the first substrate of enzymolysis, adding β-glucanase according to the ratio of 5-1000 U per gram of the first substrate of enzymolysis, and enzymolyzing for 0