EP-4003288-B1 - COSMETIC COMPOSITION WITH COMBINED FILLER AND SKIN REGENERATIVE EFFECT

Inventors

• GIULIANI, GIAMMARIA
• RINALDI, FABIO
• SPARAVIGNA, ADELE
• MARZANI, Barbara

Dates

Publication Date

20260506

Application Date

20200729

Claims (11)

1. A composition comprising a dermal filler component in combination with a peptide having biological and antiaging activity on the skin, wherein the filler component comprises a not-crosslinked hyaluronic acid or a salt thereof and a recombinant type 1 collagen protein wherein the peptide is a dipeptide selected from AlaPro, ProGly, GlyPro and mixtures thereof.
2. The composition according to claim 1 which is or is contained in a dermal filler.
3. The composition of claim 1 or 2 wherein the peptides are a mixture of AlaPro, ProGly and GlyPro.
4. The composition according to anyone of claims 1-3 further comprising an amino acid selected from Alanine, Valine, Glycine, Proline, OH-Proline, Lysine, Glutamic Acid, Glutamine, Asparagine, Leucine and mixtures thereof.
5. The composition according to anyone of claims 1-3 comprising the amino acid Alanine, Valine, Glycine, Aspartic acid, Proline, OH-Proline, Lysine, Glutamic Acid, Glutamine, Asparagine, Leucine.
6. The composition according to anyone of claims 1-5 wherein the recombinant type 1 collagen is the recombinant type I collagen α1 chain, preferably produced by transgenic silkworms.
7. The composition according to anyone of claim 1-6 wherein the hyaluronic acid is from 200 to 400kDa.
8. Cosmetic use of a composition according to anyone of claims 1-7 for improving an aesthetic aspect of an individual.
9. Cosmetic use according to claim 8 for increasing the volume or reshaping a soft tissue, especially skin of the face or neck.
10. Cosmetic use according to claim 8 for reducing the depth or ameliorating the aesthetic aspect of wrinkles, folds, corrugations, furrows, folds, in particular on the face or neck.
11. Composition comprising a dermal filler component in combination with a peptide having biological and antiaging activity on the skin, wherein the filler component comprises a not-crosslinked hyaluronic acid or a salt thereof and a recombinant type 1 collagen protein and the peptide is selected from AlaPro, ProGly, GlyPro and mixtures thereof for use in the treatment of wrinkles, folds, corrugations, furrows, by a subcutaneous injection of a cosmetically active amount of said composition.

Description

FIELD OF THE INVENTION The present invention relates to a dermo-cosmetic composition with combined filling and regenerative effect on skin. The present invention origins in the cosmetic and aesthetic fields. In particular, the present invention relates to a dermo-cosmetic composition with filler and regenerative effect on the skin, comprising the combination of a two-components dermal filler with amino acid components having biological activity on the skin. PRIOR ART Human skin aging is a multifactorial, complex and chronic biological process affecting the main skin components. Even if the skin aging mechanism is not yet fully know, recent studies have shown that the skin tends to lose the molecules responsible for binding and retaining water molecules while a reduction in the number and functionality of fibroblasts and collagen occurs. The exposure of the skin to external factors such as solar radiation, heat or cold temperatures contributes to accelerate the aging of the skin. Skin aging especially in the face of an individual manifests with a thinning of the epidermis and a loss of elasticity and with the appearance of wrinkles, furrows, atrophy, relaxation and loss of skin elasticity. In the cosmetic field, preparations containing substances with moisturizing, emollient and regenerating action are commonly used to improve the skin appearance and remedy to the signs of skin aging. However, the cosmetic effects resulting from the topical applications of these cosmetic substances is not considered fully satisfactory by the users. For this reasons, certain techniques providing for the intradermal injection of dermo-cosmetic fillers have been developed in alternative to the topical application of cosmetic substances. In aesthetic medicine, the dermal fillers are used to treat these signs of aging and to fill skin wrinkles or increase volumes. Fillers are generally polymeric products injectable subcutaneously in the vicinity of wrinkles, skin furrows and in general in areas of the body where there is a sagging skin. Once injected, they mainly carry out a mechanical action of filling the tissues, causing an action of stretching and relaxation of the skin which reduces the depth of the epidermal furrows. The fillers available on the market can be divided into two main categories, those based on biodegradable substances and those non-biodegradable. Non-biodegradable fillers are called permanent fillers because they are not absorbable and are generally encapsulated in fibrous tissues and are not subject to hydrolysis or phagocytosis. While exerting a prolonged action, however, permanent fillers have a higher risk of developing allergic and rejection reactions by the human body. Typical examples of permanent fillers include liquid silicones, silicone particles in suspension, particles of acrylic hydrogel crosslinked with hyaluronic acid, calcium hydroxyapatite, polymethylmethacrylate microspheres dispersed in collagen and formulations of polyacrylamide gel. Biodegradable fillers are called temporary because their effect wears off over time and their application needs to be renewed to maintain unchanged the filling effect. Examples of biodegradable fillers are disclosed in patent applications CN108261341 A, WO2009/024350 A2 or WO2013/036568 A1. Biodegradable fillers are generally divided into two types, the intermediate duration ones and the long duration ones. The intermediate duration ones are absorbable after a few months of implantation and are made of materials subject to degradation by enzymatic hydrolysis, such as hyaluronic acid, and therefore they gradually lose the initial ability to fill and relax the tissues. Typically, these polymers are biodegradable materials present in nature, with high biocompatibility and affinity with water. However, these polymeric matrices, such as hyaluronic acid, are rapidly hydrolysed once implanted in a tissue, and the results obtained, although appreciable from an aesthetic point of view, do not last long. In order to remedy these problems, the biodegradable polymer matrix have been modified so as to obtain polymers in crosslinked form with longer duration of action. At present, hyaluronic acids are available on the market which are treated with crosslinking agents so as to form covalent bonds which make the resulting polymer matrix less subject to enzymatic hydrolysis and enzymatic degradation, once injected into the tissues. Because these crosslinked polymer matrices are less soluble and less prone to degradation, they behave as inert substances that play a mere tissue filling function, without making a contribution to improve the conditions of the tissues that have led to the formation or accelerated the formation of the wrinkle. Some long duration of action fillers are also known which are made in synthetic polymer matrices. While having the advantage of being little biodegradable and thus having a long duration of action, these fillers are not free from drawbacks as t