EP-4596717-A3 - RECOMBINASE POLYMERASE AMPLIFICATION REAGENTS AND KITS
EP4596717A3EP 4596717 A3EP4596717 A3EP 4596717A3EP-4596717-A3
Abstract
The disclosed reagents, kits and methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. This disclosure describes reagents, kits and methods for Recombinase Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions.
Inventors
- PIEPENBURG, OLAF
- ARMES, NIALL, A.
Assignees
- Abbott Diagnostics Scarborough, Inc.
Dates
- Publication Date
- 20260506
- Application Date
- 20100607