COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS

Abstract

The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R/A, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.

Inventors

ALEKSEYEV, VIKTOR YURYEVICH
WARD, GLENN STEVEN
YAO, JIAN
AMIN, NEELAM, S.
AUGUSTYN, KATHERINE
CASCAO-PEREIRA, LUIS GUSTAVO
COLLIER, KATHERINE, D.
ESTELL, DAVID, A.
KELLIS JR., James, T
POULOSE, AYROOKARAN, J.
SOUTER, PHILIP FRANK

Assignees

DANISCO US INC
The Procter & Gamble Company

Dates

Publication Date: 20260513
Application Date: 20110505