NUCLEIC ACID CONSTRUCTS AND METHODS OF USE

Abstract

Described herein is a bidirectional nucleic acid construct comprising: a) a first segment comprising a first coding sequence for a polypeptide; and b) a second segment comprising a reverse complement of a second coding sequence for the polypeptide, wherein the second coding sequence adopts a different codon usage from that of the first coding sequence, wherein the bidirectional nucleic acid construct does not comprise a promoter that drives the expression of the first or second coding sequence; wherein the construct does not comprise a homology arm, and wherein the construct does not comprise a first and second rare-cutting endonuclease target site. Accordingly, vectors, lipid nanoparticles and host cells comprising the construct are described. Also described are methods for use of a construct, vector, and/or lipid nanoparticle in a method of expressing the polypeptide or therapeutic protein in a subject.

Inventors

FINN, Jonathan Douglas
HUANG, Hon-Ren

Assignees

Intellia Therapeutics, Inc.
Regeneron Pharmaceuticals, Inc.

Dates

Publication Date: 20260513
Application Date: 20191018