EP-4717782-A3 - SPATIAL MULTIOMICS USING IN SITU REVERSE TRANSCRIPTION
Abstract
Disclosed herein include systems, methods, compositions, and kits for determining the spatial location and copy number of targets (e.g., nucleic acid targets, cellular component targets) in a sample. There are provided, in some embodiments, substrates comprising a plurality of spatial regions. A plurality of oligonucleotide barcodes can be associated with each of the spatial regions and can comprise a predetermined spatial label. Oligonucleotide barcodes of the same spatial region can comprise the same spatial label, and oligonucleotide barcodes of the different spatial regions can comprise different spatial labels. The method can comprise contacting the substrate with a sample such that each distinct spatial region contacts a distinct spatial location of the sample. The method can comprise in situ extension (e.g., reverse transcription) of the oligonucleotide barcodes.
Inventors
- SONG, HYE-WON
- MARTIN, Jody
- NAKAMOTO, Margaret
Assignees
- Becton, Dickinson and Company
Dates
- Publication Date
- 20260506
- Application Date
- 20220831