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EP-4735563-A1 - DETERGENT COMPOSITION COMPRISING LIPASES

EP4735563A1EP 4735563 A1EP4735563 A1EP 4735563A1EP-4735563-A1

Abstract

The present invention concerns a detergent composition comprising a first lipase and a second lipase, wherein the lipases have synergistic effect on the removal of fat. In a further aspect the present invention concerns the use of the detergent composition of the invention for cleaning of a textile.

Inventors

  • DAMAGER, Iben

Assignees

  • Novozymes A/S

Dates

Publication Date
20260506
Application Date
20240619

Claims (13)

  1. 1 . A detergent composition comprising a. a first lipase having at least 60% sequence identity to SEQ ID NO: 1 b. a second lipase having at least 60% sequence identity SEQ ID NO: 3 c. at least one surfactant, and d. optionally one or more additional enzymes
  2. 2. The detergent composition according to claim 1 a, wherein the first lipase has at least 70% identity to SEQ ID NO: 1 , such as at least 80%, 85%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to SEQ ID NO: 1 .
  3. 3. The detergent composition according to claim 1 b, wherein the second lipase has at least 70% identity to SEQ ID NO: 3, such as at least 80%, 85%, 90%, 91 %, 92%, 93%, 94%, 95% identity to SEQ ID NO: 3.
  4. 4. The detergent composition according to claim 1 b, wherein the second lipase has at least 80% identity such as at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95% identity to any of SEQ ID NO: 4 or SEQ ID NO: 5
  5. 5. The detergent composition according to any of claims 1 to 4, wherein the detergent composition is in a liquid form.
  6. 6. The detergent composition according to any of claims 1 to 5, wherein the concentration in the detergent of the first lipase having at least 60% identity, such as 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% or even 100% identity to the amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 2 is in the range from 0.3 mg AEP/g detergent composition to 10 mg AEP/g detergent composition, such as 0.4 mg AEP/g detergent composition to 10 mg AEP/g detergent composition, such as 0.5 mg AEP/g detergent composition to 10 mg AEP/g detergent composition, such as 0.6 mg AEP/g detergent composition to 10 mg AEP/g detergent composition, such as 0.7 mg AEP/g detergent composition to 10 mg AEP/g detergent composition, such as 0.8 mg AEP/g detergent composition to 10 mg AEP/g detergent composition, such as from 0.3 mg AEP/g detergent composition to 8 mg AEP/g detergent composition, such as 0.4 mg AEP/g detergent composition to 8 mg AEP/g detergent composition, such as 0.5 mg AEP/g detergent composition to 8 mg AEP/g detergent composition, such as 0.6 mg AEP/g detergent composition to 8 mg AEP/g detergent composition, such as 0.7 mg AEP/g detergent composition to 8 mg AEP/g detergent composition, such as 0.8 mg AEP/g detergent composition to 8 mg AEP/g detergent composition, even more preferably in the range from 0.3 mg AEP/g detergent composition to 6 mg AEP/g detergent composition, such as 0.4 mg AEP/g detergent composition to 6 mg AEP/g detergent composition, such as 0.5 mg AEP/g detergent composition to 6 mg AEP/g detergent composition, such as 0.6 mg AEP/g detergent composition to 6 mg AEP/g detergent composition, such as 0.7 mg AEP/g detergent composition to 6 mg AEP/g detergent composition, such as 0.8 mg AEP/g detergent composition to 6 mg AEP/g detergent composition.
  7. 7. The detergent composition according to any of claims 1 to 6 comprising 0.05-20 wt% rhamnolipid, such as 1-15 wt% rhamnolipid, such as 2-10 wt% rhamnolipid, such as 3-9 wt% rhamnolipid, such as 4-8 wt% rhamnolipid.
  8. 8. A method for removal of lipid in a textile during a wash cycle comprising contacting the textile with the detergent composition of any of claims 1 to 7.
  9. 9. A washing method for textile comprising: a. Exposing a textile to a wash liquor, said wash liquor comprising detergent composition according to any of claims 1 to 7; b. completing at least one wash cycle, and c. optionally rinsing the textile.
  10. 10. The washing method according to claim 9, wherein the temperature of the wash liquor is in the range of 5°C to 90°C, or in the range of 10°C to 80°C, or in the range of 10°C to 70°C, or in the range of 10°C to 60°C, or in the range of 10°C to 50°C, or in the range of 15°C to 40°C, or in the range of 20°C to 30°C.
  11. 11. The detergent composition, method and washing method according to any of the preceding claims further comprising one or more enzymes selected from the group consisting of proteases, amylases, deoxyribonucleases, xyloglucanases, pectinases, pectin lyases, xanthan lyases, xanthan endoglucanases, peroxidases, haloperoxygenases, cellulases, licheninase, lipases, cutinases, catalases, oxidase, arabinose, galactanase and mannanases.
  12. 12. Use of the detergent composition according to any of claims 1 to 6 for removal of lipid stain on textile in a wash liquor, wherein the wash liquor comprises from about 0.2 g detergent composition/L wash liquor to about 3 g detergent composition/L wash liquor.
  13. 3. The washing method according to any of claims 9 and 10, wherein the wash liquor comprises from about 0.2 g detergent composition/L wash liquor to about 3 g detergent composition/L wash liquor.

Description

DETERGENT COMPOSITION COMPRISING LIPASES Reference to a Sequence Listing This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference. Field of the Invention The present invention concerns a detergent composition comprising a first lipase and a second lipase, wherein the lipases have synergistic effect on the removal of fat. In a further aspect the present invention concerns the use of the detergent composition of the invention for cleaning of a textile. Background The ability of a detergent to remove stains from the surface of textiles is an obvious care- about for the customer and various surfactant ingredients play a role in that process. However, there is a desire to reduce the amount of detergent used in household care for a number of reasons. One reason is that some of the ingredients in detergents are derived from petrochemical resources and face scrutiny due to environmental concerns, most of all for not being sustainable because they are from a non-renewable source and are poorly biodegradable or even persistent in the environment. Another reason is that lowering the detergent concentration in the wash liquor may reduce production cost and will ultimately lead to less transportation of detergents and consequently less burden on the environment. This trend toward compaction of detergents and reduced in-wash concentration of surfactants requires the development of solutions to ensure continued performance of the detergents, including new enzymes and new use of enzymes. Lipases are included in some detergents to improve fat (triglyceride) removal. Most commercially available lipases for use in detergent release short-chain fatty acids (e.g., butyric acid and hexanoic acid) when degrading lipid stains, leading to malodor perception. The majority of these lipases have a high degree of identity to the lipase having SEQ ID NO: 3 from Thermomyces lanuginosus and for the ease of reference they will be referred to as TLL-like lipases. The dosage of TLL-like lipase is often limited in laundry detergents by the highest acceptable level of malodors, in particular under wash conditions with low detergent load since TLL-like lipase will be left on the stain after wash and the amount of ester-free perfume system in the detergent formulation is limited due to the lower detergent concentration. WO 2016/050661 (Novozymes A/S) discloses TLL-like lipase variants which develop a low level or reduced level of malodor as compared to the parent enzyme. WO 2017/001673 (Novozymes A/S) relates to methods of reducing malodor during lipid stain removal. A different group of lipases has been described by Bertolini et al (Eur. J. Biochem. 228, 863- 869 (1995)), namely the Geotrichum candidum lipase I (GCL 1). The GCL 1 lipase is highly specific for long-chain length unsaturated fatty acids (such as linoleic acid and alpha-linoleic acid) and creates thus less odor compared to TLL-like lipase when used in laundry detergents. For the ease of reference, they will be referred to as GCL 1 -like lipases. WO 2022/162043 discloses detergent composition comprising GCL 1 lipase. SEQ ID NO: 2 is disclosed in Shimada et al: cDNA Molecular Cloning of Geotrichum candidum Liase, The Journal of Biochemistry, Volume 106, Issue 3, September 1989, Pages 383-388, (world wide web: doi.org/10.1093/oxfordjournals.jbchem.a122862) and Swisss-Prot: P17573. SEQ ID NO: 1 is a variant of SEQ ID NO: 2 with two substitutions: S509A and K511 R SEQ ID NO: 3 is a lipase from Thermomyces lanuginosus The prior art does not disclose the synergistic effect of use of GCL 1 -like lipase and TLL-like lipase in detergent compositions. Summary of the Invention Many petrochemically derived compounds present in detergents are not sustainable because they are derived from a non-renewable source and are poorly biodegradable or even persistent in the environment. The inventors of the present invention have surprisingly found that the combined use of GCL 1 -like lipases that are specific for long-chain length unsaturated fatty acids (e.g., SEQ ID NO: 1) and TLL-like lipase (e.g., a variant of SEQ ID NO: 3) has a strong synergistic effect on the degradation of lipid stains during wash. This is important as it allows for reduced detergent concentrations without reduction of lipid removal. In addition to being produced from a renewable agricultural source, and in contrast to many detergent ingredients, lipases are naturally found in the environment and readily biodegradable. The replacement of detergent ingredients with GCL 1-like lipases and TLL-like lipases addresses the United Nations’ Sustainable Development Goals, in particular Goal 12 “Responsible consumption and production”: replacing detergent ingredients with GCL 1-like lipases and TLL-like lipases allows the detergent producer - and thus the end user - to move from a fossil feedstock to a renewable feedstock and reduce the volume of persistent chemicals emitted to th