EP-4735608-A1 - A NOVEL QTL FOR PURPLE COLOR IN BRASSICA OLERACEA

Abstract

The present disclosure provides broccoli plants ( Brassica oleracea var. italica' ) exhibiting a novel purple phenotype. Such plants may comprise novel introgressed genomic regions associated with the purple phenotype from Brassica oleracea var. capitata f. rubra . In certain aspects, compositions, including novel polymorphic markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a purple phenotype are provided.

Inventors

• BOON, MEINARDUS
• WANG, Sufei

Assignees

• Seminis Vegetable Seeds, Inc.

Dates

Publication Date

20260506

Application Date

20240626

Claims (1)

1. BCS226311 FC What is Claimed is: 1. An elite broccoli plant comprising a recombinant chromosomal segment on chromosome 6, wherein said recombinant chromosomal segment comprises an allele from Brassica oleracea var. capitata f. rubra that confers to said plant a purple phenotype compared to a plant not comprising said allele. 2. The plant of claim 1, wherein said recombinant chromosomal segment comprises a marker selected from the group consisting of marker M1 (SEQ ID NO:1), marker M2 (SEQ ID NO:2), marker M3 (SEQ ID NO:3), and marker M4 (SEQ ID NO:4) on chromosome 6. 3. The plant of claim 1, wherein said plant is homozygous for said recombinant chromosomal segment. 4. The plant of claim 1, wherein said plant is heterozygous for said recombinant chromosomal segment. 5. A plant part of the plant of claim 1, wherein said plant part comprises said recombinant chromosomal segment. 6. The plant part of claim 5, wherein said plant part is a cell, a seed, a root, a stem, a stalk, a leaf, a bud, a flower, a floret, a head, an ovule, or pollen. 7. A seed that produces the plant of claim 1. 8. The plant of claim 1, wherein a sample of seed comprising said recombinant chromosomal segment has been deposited under NCMA Accession No.202211063. 9. A recombinant DNA segment comprising an allele from Brassica oleracea var. capitata f. rubra, wherein said allele confers to a broccoli plant a purple phenotype when present in said plant. 10. The recombinant DNA segment of claim 9, wherein said recombinant DNA segment comprises a sequence selected from the group consisting of SEQ ID NOs:1, 2, 3, and 4. 11. The recombinant DNA segment of claim 9, further defined as comprised within a plant, plant part, plant cell, or seed. 12. The recombinant DNA segment of claim 9, wherein a sample of seed comprising said recombinant DNA segment has been deposited under NCMA Accession No.202211063. BCS226311 FC 13. A method of producing an elite broccoli plant exhibiting a purple phenotype, comprising introgressing into said plant an allele from Brassica oleracea var. capitata f. rubra within a recombinant chromosomal segment flanked in the genome of said plant by marker M1 (SEQ ID NO:1) and marker M4 (SEQ ID NO:4) on chromosome 6, wherein said allele confers to said plant a purple phenotype compared to a plant not comprising said allele, and wherein said introgressing comprises marker-assisted selection. 14. The method of claim 13, wherein said introgressing comprises: a) crossing a plant comprising said chromosomal segment with itself or with a broccoli plant of a different genotype to produce one or more progeny plants; and b) selecting a progeny plant comprising said chromosomal segment. 15. The method of claim 14, wherein selecting a progeny plant comprises detecting nucleic acids comprising marker locus M1 (SEQ ID NO:1), marker locus M2 (SEQ ID NO:2), marker locus M3 (SEQ ID NO:3), or marker locus M4 (SEQ ID NO:4). 16. The method of claim 14, wherein the progeny plant is an F 2 -F 6 progeny plant. 17. The method of claim 14, wherein said crossing comprises backcrossing. 18. A broccoli plant obtainable by the method of claim 13. 19. A method of producing food or feed comprising obtaining a plant according to claim 1 or 18, or a part thereof, and producing said food or feed from said plant or part thereof. 20. A method of selecting a broccoli plant exhibiting a purple phenotype, comprising: a) crossing the broccoli plant of claim 1 with itself or with a second broccoli plant of a different genotype to produce one or more progeny plants; and b) selecting a progeny plant comprising said recombinant chromosomal segment. 21. The method of claim 20, wherein selecting said progeny plant comprises detecting a marker locus genetically linked to said recombinant chromosomal segment. 22. The method of claim 21, wherein selecting said progeny plant comprises detecting a marker locus within or genetically linked to a chromosomal segment flanked in the genome of said plant by marker locus M1 (SEQ ID NO:1) and marker locus M4 (SEQ ID NO:4) on chromosome 6. BCS226311 FC 23. The method of claim 20, wherein selecting a progeny plant comprises detecting nucleic acids comprising marker locus M1 (SEQ ID NO:1), marker locus M2 (SEQ ID NO:2), marker locus M3 (SEQ ID NO:3), or marker locus M4 (SEQ ID NO:4). 24. The method of claim 20, wherein said progeny plant is an F 2 -F 6 progeny plant. 25. The method of claim 20, wherein producing said progeny plant comprises backcrossing. 26. A broccoli plant obtainable by the method of claim 20. 27. A cell according to claim 6. 28. A tissue culture comprising the cell of claim 6. 29. A method of selecting a broccoli plant or broccoli seed, said method comprising: a) detecting in a population of broccoli plants or broccoli seeds a broccoli plant or broccoli seed comprising an allele from Brassica oleracea var. capitata f. rubra that confers to said plant a purple phenotype compared to a plant not comprising said allele. b) selecting said broccoli plant or broccoli seed comprising said allele from Brassica oleracea var. capitata f. rubra that confers to said plant a purple phenotype. 30. The method of claim 29, wherein said allele is located on chromosome 6. 31. The method of claim 29, wherein said allele is linked to any marker selected from the group consisting of marker M1 (SEQ ID NO:1), marker M2 (SEQ ID NO:2), marker M3 (SEQ ID NO:3), and marker M4 (SEQ ID NO:4) on chromosome 6. 32. The method of any one of claims 29 to 31, wherein said plant is homozygous for said allele from Brassica oleracea var. capitata f. rubra. 33. The method of any one of claims 29 to 31, wherein said plant is heterozygous for said allele from Brassica oleracea var. capitata f. rubra. 34. The method of any one of claims 31 to 33, wherein the marker M1 comprises a C at position 61 of SEQ ID NO:1; M2 comprises a C at position 61 of SEQ ID NO:2; M3 comprises a T at position 61 of SEQ ID NO:3; and M4 comprises a G at position 61 of SEQ ID NO:4. BCS226311 FC 35. The method of any one of claims 29 to 33, wherein said broccoli plant or broccoli seed is an elite plant or a seed of an elite plant. 36. A plant identifiable by the method according to any one of claims 29 to 35. 37. A nucleic acid molecule comprising an allele conferring to a broccoli plant a purple phenotype, wherein the nucleic acid molecule is obtainable or can be obtained from seed deposited under NCMA Accession No.202211063, and wherein the nucleic acid molecule comprises at least one marker selected from the group consisting of marker M1 (SEQ ID NO:1), marker M2 (SEQ ID NO:2), marker M3 (SEQ ID NO:3), and marker M4 (SEQ ID NO:4). 38. The nucleic acid molecule according to claim 37, wherein the at least one marker comprises: SEQ ID NO:1 comprising a C at position 61; SEQ ID NO:2 comprising a C at position 61; SEQ ID NO:3 comprising a T at position 61; or SEQ ID NO:4 comprising a G at position 61.

Description

BCS226311 FC APPLICATION FOR LETTERS PATENT for A NOVEL QTL FOR PURPLE COLOR IN BRASSICA OLERACEA by MEINARDUS BOON and SUFEI WANG BCS226311 FC CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority to U.S. Provisional Patent Application No. 63/510,497, filed June 27, 2023, the content of which is incorporated herein by reference in its entirety. INCORPORATION OF SEQUENCE LISTING [0002] The sequence listing contained in the file named "BCS226311_US01.xml", which is 7 kilobytes as measured in Microsoft Windows operating system and was created on May 28, 2024, is filed electronically herewith and incorporated herein by reference. FIELD [0003] The present disclosure relates to the field of agriculture and, more specifically, to methods and compositions for producing broccoli plants with a unique purple phenotype. The purple phenotype is exhibited in broccoli plants and tissues including but not limited to the florets, main head, stems, beads, leaf petiole, and leaf midrib. BACKGROUND [0004] One of the major goals in modern plant breeding programs is to improve existing plant varieties, especially vegetable crops. In addition to identifying alleles that confer a desirable phenotype, specific markers linked to such alleles facilitate their introduction into cultivated lines. Marker-assisted selection (MAS) in plant breeding methods has made it possible to select plants based on genetic markers linked to traits of interest, in this case, a purple phenotype. However, identification of markers for tracking and/or introducing desirable traits in plants requires significant effort and as such, the markers are often unavailable even if the gene associated with the trait has been characterized. The difficulty in identifying markers is also complicated by factors such as polygenic or quantitative inheritance, epistasis and an often incomplete understanding of the genetic background underlying expression of a desired phenotype. SUMMARY [0005] In one aspect, the present disclosure provides an elite broccoli plant comprising a recombinant chromosomal segment on chromosome 6, wherein said recombinant chromosomal segment comprises an allele from Brassica oleracea var. capitata f. rubra that confers to said plant a purple phenotype compared to a plant not comprising said allele. In some embodiments, said BCS226311 FC recombinant chromosomal segment comprises a marker selected from the group consisting of marker M1 (SEQ ID NO:1), marker M2 (SEQ ID NO:2), marker M3 (SEQ ID NO:3), and marker M4 (SEQ ID NO:4) on chromosome 6. In some embodiments, said plant is homozygous for said recombinant chromosomal segment. In other embodiments, said plant is heterozygous for said recombinant chromosomal segment. In further embodiments, a sample of seed comprising said recombinant chromosomal segment has been deposited under NCMA Accession No.202211063. [0006] In addition, the present disclosure provides a plant part of an elite broccoli plant comprising a recombinant chromosomal segment on chromosome 6, wherein said recombinant chromosomal segment comprises an allele from Brassica oleracea var. capitata f. rubra that confers to said plant a purple phenotype compared to a plant not comprising said allele. In some embodiments, said plant part is a cell, a seed, a root, a stem, a stalk, a leaf, a bud, a flower, a floret, a head, an ovule, or pollen. The present disclosure also provides a seed that produces the broccoli plants described herein. [0007] The present disclosure provides a recombinant DNA segment (which may be referred to as a nucleic acid molecule) comprising an allele from Brassica oleracea var. capitata f. rubra, wherein said allele confers to a broccoli plant a purple phenotype when present in said plant. In some embodiments, said recombinant DNA segment comprises a sequence selected from the group consisting of SEQ ID NOs:1, 2, 3, and 4. In other embodiments, said recombinant DNA segment is further defined as comprised within a plant, plant part, plant cell, or seed. In further embodiments, a sample of seed comprising said recombinant DNA segment has been deposited under NCMA Accession No.202211063. [0008] In yet another aspect, the present disclosure provides a nucleic acid molecule comprising an allele conferring to a broccoli plant a purple phenotype, wherein the nucleic acid molecule is obtainable or can be obtained from seed deposited under NCMA Accession No.202211063, and wherein the nucleic acid molecule comprises at least one marker selected from the group consisting of marker M1 (SEQ ID NO:1), marker M2 (SEQ ID NO:2), marker M3 (SEQ ID NO:3), and marker M4 (SEQ ID NO:4). In further embodiments, SEQ ID NO:1 comprises a C at position 61; SEQ ID NO:2 comprises a C at position 61; SEQ ID NO:3 comprises a T at position 61, and SEQ ID NO:4 comprises a G at position 61. [0009] In addition, the present disclosure provides a cell of an elite broccoli plant comprising a recombinant chromosomal segment