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EP-4736612-A1 - BIOCHAR SEED GEL FOR VEGETATION RESTORATION IN SALINE-ALKALI LAND, PREPARATION METHOD THEREFOR AND USE THEREOF

EP4736612A1EP 4736612 A1EP4736612 A1EP 4736612A1EP-4736612-A1

Abstract

The present invention discloses a biochar-seed gel for vegetation rehabilitation in saline-alkali soil, a preparation method thereof, and an application thereof and belongs to the technical field of vegetation rehabilitation. The biochar-seed gel of the present invention includes a water-retaining hydrogel, effective nutrient components, and a structural maintenance agent, wherein the water-retaining hydrogel is polyacrylamide; the effective nutrient components comprise Bacillus subtilis inoculant, brassinolide, chitosan oligosaccharide, polyaspartic acid, and compound fertilizer; and the structural maintenance agent comprises biochar, clay, chitosan, and Gleditsia sinensis powder. In the present invention, the biochar-seed gel forms a microenvironment on the soil surface, mitigating stress caused by high-salinity soil conditions. The gel exhibits strong water-retention capacity, effectively maintaining moisture to support seed and seedling growth while alleviating water deficiency resulting from soil compaction.

Inventors

  • The designation of the inventor has not yet been filed

Assignees

  • Beijing Normal University

Dates

Publication Date
20260506
Application Date
20240925

Claims (10)

  1. A biochar-seed gel for vegetation rehabilitation in saline-alkali soil, comprising the following components: a water-retaining hydrogel, effective nutrient components, and a structural maintenance agent; wherein the water-retaining hydrogel is polyacrylamide; the effective nutrient components comprise Bacillus subtilis inoculant, brassinolide, chitosan oligosaccharide, polyaspartic acid, and compound fertilizer; and the structural maintenance agent comprises biochar, clay, chitosan, and Gleditsia sinensis powder.
  2. The biochar-seed gel according to claim 1, wherein a weight ratio of the water-retaining hydrogel, the effective nutrient components, and the structure-maintaining agent is 1-10:1-5:1-10.
  3. The biochar-seed gel according to claim 2, wherein a weight ratio of the Bacillus subtilis inoculant, brassinolide, chitosan oligosaccharide, polyaspartic acid, and compound fertilizer is 3-5:1-2:5-10:1-2:3-5.
  4. The biochar-seed gel according to claim 3, wherein a weight ratio of the biochar, clay, chitosan, and Gleditsia sinensis powder is 15-30:50-70:5-10:1-3.
  5. The biochar-seed gel according to claim 4, wherein a bacterial concentration of the Bacillus subtilis inoculant is 5×10 10 -1×10 12 cfu/g; the compound fertilizer is a high-phosphorus-potassium fertilizer; the biochar is reed-derived biochar with a particle size of ≥200 mesh; and the clay is in-situ soil from the ecological restoration area, dried at 170-190 °C for 20-24 hours, with a particle size of ≥100 mesh.
  6. A preparation method of the biochar-seed gel according to claims 1-5, comprising the following steps: 1) mixing the Bacillus subtilis inoculant, brassinolide, chitosan oligosaccharide, polyaspartic acid, and compound fertilizer to obtain the effective nutrient components; 2) mixing the biochar, clay, chitosan, and Gleditsia sinensis powder to obtain the structural maintenance agent; and 3) mixing the water-retaining hydrogel, the effective nutrient components, and the structural maintenance agent to obtain the biochar-seed gel.
  7. The preparation method according to claim 6, wherein the biochar-seed gel is stored in a sealed environment at 3-5 °C.
  8. An application of the biochar-seed gel according to any one of claims 1-5 or a biochar-seed gel prepared by the preparation method according to any one of claims 6-7 for vegetation rehabilitation in saline-alkali soil.
  9. The application according to claim 8, wherein the biochar seed gel is suitable for halophytes with a seed diameter of less than 5 mm.
  10. The application according to claim 9, wherein the application comprises the following steps: (1) mixing seeds after being soaked and pre-germinated with the biochar-seed gel at a mass ratio of 1:3 to 1:5 to obtain a mixture of seeds and gel; and (2) adding water in an amount of 8 to 12 times the mass of the mixture of seeds and gel obtained in step (1), standing for 20 to 28 hours, and then spraying onto saline-alkali soil.

Description

TECHNICAL FIELD The present invention belongs to the technical field of vegetation rehabilitation and specifically relates to a biochar-seed gel for vegetation rehabilitation in saline-alkali soil, a preparation method thereof, and an application thereof. BACKGROUND Saline-alkali soil refers to a soil containing high concentrations of soluble salts and alkaline substances. Such a soil environment poses a severe threat to agricultural production and ecosystems, resulting in a significant decline in crop yield. In saline-alkali environments, high concentrations of soil salt ions induce soil compaction, consequently causing poor soil structure and fertility degradation; and excessively high soil salinity readily interferes with plant nutrient acquisition and translocation, thereby inducing nutrient imbalance. This leads to reduced vegetation productivity, subsequently triggering degradation of ecological functions in surrounding environments, which poses a severe threat to local ecological security. Vegetation rehabilitation of saline-alkali soils is currently the predominant approach in ecological restoration of such soils. Large-scale propagation of vegetation seeds and propagules are critical steps in the vegetation rehabilitation of saline-alkali soil. Particularly for the most common annual plants in saline-alkali soil, it is essential to maintain a vegetation scale comparable to that of the previous year. Following vegetation degradation, where there is insufficient seed source availability and a lack of suitable soil conditions to support plant community colonization in the subsequent growing season, active supplementation of seeds and juvenile plants is required. Conventional vegetation rehabilitation methods primarily comprise sowing, plant species selection and breeding, and transplanting. By performing large-scale propagation on the seeds and propagules of plants, the survival rate of plants in saline-alkali soil is effectively enhanced, thereby achieving restoration of the local vegetation community. However, under high-salinity and arid stress conditions in saline-alkali soils, vegetative growth and germination are conventionally severely restricted. Even the colonization and growth of stress-tolerant plants can be affected to some extent, which poses significant challenges to the ecological restoration of saline-alkali soils. Therefore, developing cost-effective ecological restoration technologies adapted to the special stress conditions of saline-alkali lands is a critical technical challenge that urgently needs to be addressed. The application of seed improvement technologies in agriculture has reached considerable maturity, wherein seed coating technology is a widely adopted measure to enhance seed stress tolerance and survival rates. By treating the seed exterior with coating agents, the germination rate, growth velocity and stress resistance of the seeds are significantly improved. By applying a coating agent to the exterior surface of seeds, the germination rate, growth speed, and stress resistance are enhanced. With the continuous development of ecological restoration, facing issues such as insufficient natural germplasm resources and relatively low germination rates of natural seeds under stressful conditions, seed coating technology has been gradually introduced into the field of vegetation rehabilitation. For the specific stressful environment of saline-alkali soil, improving and optimizing seed coating technology is particularly critical, yet relevant techniques remain lacking. Conventional seed coating technologies are designed for farmland applications, but the effectiveness is limited in saline-alkali soils due to constraints such as high salinity and drought. It is necessary to propose targeted technical solutions to overcome these limitations, with stricter requirements for the coating material's performance, particularly in salt reduction and water retention. Furthermore, conventional agricultural seed coating technologies conflict with environmentally friendly principles due to their inclusion of bactericides, pesticides, and similar additives. Excessive synthetic organic and inorganic compounds contribute to environmental pollution, exacerbating the degradation of local fragile ecosystems. Accordingly, the present invention is proposed to address the requirements of low cost, pollution-free operation, salt reduction, and drought resistance. SUMMARY An objective of the present invention is to provide a biochar-seed gel for vegetation rehabilitation in saline-alkali soil, a preparation method thereof, and an application thereof, to overcome the high-salinity and drought stress conditions in saline-alkali soil, thereby improving seed germination rates and plant stress resistance. To achieve the above objective, the present invention provides the following technical solutions. The present invention provides a biochar-seed gel for vegetation rehabilitation in saline-alkali soil, inc