EP-4736868-A2 - COMPOSITION FOR PREVENTING, AMELIORATING, OR TREATING DISEASE CAUSED BY PROTEIN NITRATION COMPRISING PEPTIDE WITH TERMINAL TYROSINE AS EFFECTIVE COMPONENT

Abstract

According to the present invention, the peptide with terminal tyrosine has an excellent effect on inhibiting protein nitration and also an excellent effect on preventing, ameliorating, or treating disease symptoms in chronic immobilization stress-induced depression/cognitive impairment model, Alzheimer's disease model, epileptic seizure model, stroke model, type 2 diabetes model, acute renal failure model, or hyperammonemia model.

Inventors

• KIM, HYUN JOON
• KIM, YOUNG BUM
• KANG, JAE SOON
• JUNG, Soonwoong
• SONG, Miyoung
• BAEK, JI HYEONG
• PARK, SANG WON
• KIM, HWAJIN
• YOO, DAE YOUNG

Assignees

• Industry-Academic Cooperation Foundation Gyeongsang National University

Dates

Publication Date

20260506

Application Date

20220223

Claims (4)

1. A composition for preventing, ameliorating or treating one or more diseases selected from the group consisting of type 2 diabetes, diabetic nephropathy, sarcopenia, dyslipidemia, obesity and non-alcoholic fatty liver diseases, the composition comprising tyrosine-glutamine (YQ) or a salt thereof.
2. The composition according to Claim 1, wherein the composition inhibits nitration of one or more proteins selected from the group consisting of glutamine synthetase, insulin receptor β subunit, Mn superoxide dismutase, heat shock protein 60, Cu/Zn superoxide dismutase, and catalase.
3. The composition according to Claim 1, wherein the composition reduces reactive oxygen species (ROS), or reactive nitrogen species (RNS), or oxygen species (ROS) and reactive nitrogen species (RNS).
4. The composition according to Claim 1, wherein the composition removes the nitro group from nitrotyrosine in a protein with a tyrosine moiety.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing, ameliorating, or treating a disease caused by protein nitration comprising a peptide with terminal tyrosine as effective component. BACKGROUND ART Oxidative stress is known to occur when oxygen generated during cellular metabolism acts as free radicals, causing damage to cells and leading to various diseases. In particular, when reactive oxygen species (ROS) or reactive nitrogen species (RNS) present within cells lead to the generation of peroxynitrite (ONOO-) at tyrosine included in a protein, protein nitration is caused. Protein nitration is considered a phenomenon accompanied in oxidative processes, and when specific proteins undergo nitration, their structure can be altered, leading to a decrease in activity or loss of normal function, ultimately contributing to the development of various diseases. Protein nitration has been reported to be closely related to signal transductions in cell, diseases caused by inflammatory response, neurodegenerative diseases, aging, and others, and also recently has been implicated in conditions such as asthma, diabetes, cancer, chronic stress-induced depression, type 2 diabetes, and acute renal diseases. Therefore, research on protein nitration plays a crucial role in both biological and clinical contexts, and there is a need for studies focused on developing substances that can inhibit the protein nitration. Meanwhile, Korean Patent Registration No. 1897400 discloses a "Composition for inhibiting the activity of tyrosine decarboxylase and a method for producing fermented food using the same", and Korean Patent Publication No. 2018-0021746 discloses a "Method for purifying aromatic compounds nitrated by nitration process." However, there is no description made regarding the "composition for preventing, ameliorating, or treating a disease caused by protein nitration comprising a peptide with terminal tyrosine as effective component" as described in the present invention. DETAILED DESCRIPTION OF THE INVENTION TECHNICAL PROBLEMS TO BE SOLVED The present invention has been devised under the aforementioned circumstances, and the inventors provide a composition for preventing, ameliorating, or treating a disease caused by protein nitration comprising a peptide with terminal tyrosine as effective component. By finding that the peptide with terminal tyrosine, which is the effective component of the present invention, inhibits protein nitration and also can prevent, ameliorate, or treat disease symptoms in chronic restraint stress-induced depression/cognitive impairment model, Alzheimer's disease model, epileptic seizure model, stroke model, type 2 diabetes model, acute renal failure model, or hyperammonemia model, the present invention is completed. TECHNICAL MEANS FOR SOLVING THE PROBLEMS To achieve the purpose described in the above, the present invention provides a functional health food composition for preventing or ameliorating a disease caused by protein nitration comprising a peptide with terminal tyrosine or a salt thereof, which is acceptable for use in food, as effective component. The present invention further provides a pharmaceutical composition for preventing or treating a disease caused by protein nitration comprising a peptide with terminal tyrosine or a pharmaceutically acceptable salt thereof as effective component. The present invention further provides a composition for inhibiting protein nitration comprising a peptide with terminal tyrosine or a pharmaceutically acceptable salt thereof as effective component. The present invention further provides a method for removing a nitro group from nitrotyrosine by treating a protein having nitrotyrosine with a peptide with terminal tyrosine. The present invention further provides a functional health food composition for preventing or ameliorating a disease caused by an increase in reactive oxygen species/reactive nitrogen species comprising a peptide with terminal tyrosine or a salt thereof, which is acceptable for use in food, as effective component. The present invention still further provides a pharmaceutical composition for preventing or treating a disease caused by an increase in reactive oxygen species/reactive nitrogen species comprising a peptide with terminal tyrosine or a pharmaceutically acceptable salt thereof as effective component. ADVANTAGEOUS EFFECT OF THE INVENTION The peptide with terminal tyrosine, i.e., a peptide which has tyrosine on at least one peptide chain terminal, has an excellent effect of inhibiting the protein nitration, reducing the nitration level of tyrosine in glutamine synthetase that has been increased by stress, and increasing the activity of glutamine synthetase that has been decreased by stress, and also an excellent effect of preventing, ameliorating, or treating disease symptoms in chronic restraint stress-induced depression/cognitive impairment model, Alzheimer's disease model, epileptic seizure mode