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EP-4737897-A1 - DETECTION MEMBER FOR OBJECT TO BE DETECTED AND METHOD FOR DETECTING OBJECT TO BE DETECTED USING SAME

EP4737897A1EP 4737897 A1EP4737897 A1EP 4737897A1EP-4737897-A1

Abstract

The present invention provides a detection member for an agent to be detected, the detection member including a carrier capable of aggregation on which a DNA aptamer that specifically binds to the agent to be detected is carried, and an antibody that binds to the agent to be detected and has a labeling group that emits light of a specific wavelength upon irradiation with light of a predetermined wavelength, as well as a method for detecting an agent to be detected using the detection member. The agent to be detected may be a virus, a germ, or a bacterium. The virus may be a coronavirus, an influenza virus, a norovirus, or an avian influenza virus.

Inventors

  • TAKAMURA, TAKEJI
  • OTA, MIKI
  • AOYAGI, TETSUJI

Assignees

  • Aoyagi, Tetsuji

Dates

Publication Date
20260506
Application Date
20240701

Claims (15)

  1. A detection member for an agent to be detected, comprising: a carrier capable of aggregation and carrying a DNA aptamer that specifically binds to the agent to be detected; and an antibody that binds to the agent to be detected and has a labeling group that emits light of a specific wavelength under a predetermined condition.
  2. The detection member for an agent to be detected according to Claim 1, wherein the agent to be detected is a virus, a germ, or a bacterium.
  3. The detection member for an agent to be detected according to Claim 2, wherein the virus is a coronavirus, an influenza virus, a norovirus, or an avian influenza virus.
  4. The detection member for an agent to be detected according to Claim 1, wherein the carrier capable of aggregation is magnetic beads or gold nanoparticles.
  5. The detection member for an agent to be detected according to Claim 1, wherein the antibody having a labeling group that emits light of a specific wavelength upon irradiation with light of a predetermined wavelength is an antibody modified with a fluorescent functional group.
  6. The detection member for an agent to be detected according to Claim 1, wherein the detection member is an inner sheet of a mask that is provided with a detection patch containing the carrier and the antibody.
  7. The detection member for an agent to be detected according to Claim 1, wherein the detection member is a wiper that includes a detection reagent containing the carrier and the antibody and that wipes a contacted surface of a detection target that may contain the agent to be detected or that the agent to be detected may adhere to.
  8. The detection member for an agent to be detected according to Claim 1, wherein the detection member is a detection reagent that contains the carrier and the antibody and that is sprayed onto a wiper after wiping a contacted surface of a detection target that may contain the agent to be detected or that the agent to be detected may adhere to.
  9. The detection member for an agent to be detected according to Claim 1, wherein the detection member is a saliva test stick that is provided with a detection patch containing the carrier and the antibody.
  10. The detection member for an agent to be detected according to Claim 1, wherein the detection member is a detection display member that is attached to an air conditioner.
  11. The detection member for an agent to be detected according to Claim 1, wherein the detection member is a detection display member that is attached to a desktop humidifier.
  12. A method for detecting an agent to be detected using a detection member for an agent to be detected comprising: a carrier capable of aggregation and carrying a DNA aptamer that specifically binds to the agent to be detected; and an antibody that binds to the agent to be detected and has a labeling group that emits light of a specific wavelength under a predetermined condition, the method comprising: a step of bringing the detection member into contact with a detection target that may contain the agent to be detected or that the agent to be detected may adhere to; a step of aggregating and collecting the carrier that is contained in the contacted detection member and carrying the DNA aptamer specifically bound to the agent to be detected; a step of bringing the collected carrier having the agent to be detected bound thereto into contact with an antibody that binds to the agent to be detected and is labeled with a labeling group that emits light of a specific wavelength upon irradiation with light of a predetermined wavelength, to form a complex of the agent to be detected and the labeled antibody; and a step of irradiating the formed complex of the agent to be detected and the labeled antibody with light of a predetermined wavelength and detecting the emitted light to detect the agent to be detected.
  13. The method for detecting an agent to be detected according to Claim 12, wherein in the step of collecting the aggregated carrier, the carrier is collected using an instrument having a magnet.
  14. The method for detecting an agent to be detected according to Claim 12, wherein in the step of detecting the agent to be detected, light of a predetermined wavelength is irradiated onto a detection patch that contains the carrier and the antibody and is provided in an inner sheet of a mask.
  15. The method for detecting an agent to be detected according to Claim 12, wherein in the step of forming the complex of the agent to be detected and the antibody, the wiper contains the antibody, and a detection reagent containing a carrier which carries a DNA aptamer that specifically binds to the agent to be detected is sprayed onto the wiper after wiping a contacted surface of a detection target that may contain the agent to be detected or that the agent to be detected adhere to, thereby forming the complex of the agent to be detected and the antibody.

Description

[Technical Field] The present invention relates to a detection member for an agent to be detected and to a method for detecting an agent to be detected using the detection member. [Background Art] Pathogenic viruses exemplified by an influenza virus and a norovirus are so small that their presence cannot be visually recognized in an ordinary living environment. Such invisibility of viruses increases anxiety and fear in the society and expands the spread of a disease. In particular, for viruses that are transmitted through the air, the fear due to "being invisible to the eyes" is difficult to overcome. If pathogenic viruses that are present in the air within a living space, an office, a vehicle, and the like, or that adhere to clothing or a mask, could be visualized or detected easily and with high sensitivity without requiring specialized knowledge or operational techniques of biochemical analysis, it would become possible to suppress and prevent viral infection and the spread of a disease. For the detection of such viruses, in recent years, a relatively simple inspection technique for an influenza virus using immunochromatography, such as the technique disclosed in PTL 1, has been employed. [Citation List] [Patent Literature] [PTL 1] Japanese Unexamined Patent Application, Publication No. 2008-275511 [Summary of Invention] [Technical Problem] A method for detecting an influenza virus using immunochromatography is simple, but for the detection of the virus, it is required that the viruses are present at a concentration of about 103 to 104 pfu/mL. Therefore, the detection sensitivity at an early stage when the amount of virus present is small is not necessarily high. In order to suppress infection by pathogens and the spread of a disease, there has been a strong demand for a method that can visualize rapidly and with high sensitivity harmful agents to the human body, such as the viruses that are present in the air within a living space, an office, or a vehicle, or that adhere to clothing or a mask. The present invention has been made in view of such circumstances, and an object of the present invention is to provide a detection member for an agent to be detected and a method for detecting an agent to be detected using the detection member, which make it possible to visualize the presence of an agent to be detected, within a short time and by a simple technique without using a complicated detection method or a device. [Solution to Problem] To solve the above problem, the detection member for an agent to be detected and the method for detecting an agent to be detected using the detection member according to the present invention employ the following solutions. A first embodiment of the present invention provides a detection member for an agent to be detected, the detection member including a carrier capable of aggregation and carrying a DNA aptamer that specifically binds to the agent to be detected, and an antibody that binds to the agent to be detected and has a labeling group that emits light of a specific wavelength under a predetermined condition. In the first embodiment, the agent to be detected may be a virus, a germ, or a bacterium. The virus may be a novel coronavirus, an influenza virus, a norovirus, or an avian influenza virus. In the first embodiment, the carrier capable of aggregation may be magnetic beads or gold nanoparticles. In the first embodiment, the antibody having a labeling group that emits light of a specific wavelength under a predetermined condition may be an antibody modified with a fluorescent functional group. In the first embodiment, the detection member may be an inner sheet of a mask that is provided with a detection patch containing the carrier and the antibody. In the first embodiment, the detection member may be a wiper that includes a detection reagent containing the carrier and the antibody and that wipes a contacted surface of a detection target that may contains or that the agent to be detected may adhere to. In the first embodiment, the detection member may be a detection reagent that is sprayed onto a wiper after wiping a contacted surface of a detection target that may contain the agent to be detected or that the agent to be detected may adhere to. In the first embodiment, the inner sheet of the mask or the wiper may contain moisture at a predetermined ratio. In the first embodiment, the detection member may be a saliva test stick that is provided with a detection patch containing the carrier and the antibody. In the first embodiment, the detection member may be a detection display member that is attached to an air conditioner. In the first embodiment, the detection member may be a detection display member that is attached to a desktop humidifier. A second embodiment of the present invention provides a method for detecting an agent to be detected using the detection member according to the first embodiment. In the second embodiment, the method includes a step of bringi