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EP-4739139-A1 - COMPOSITIONS CONTAINING PEROXYACIDS TO CONTROL PATHOGEN GROWTH IN ANIMAL FEED AND RELATED METHODS

EP4739139A1EP 4739139 A1EP4739139 A1EP 4739139A1EP-4739139-A1

Abstract

The present invention relates to the use of compositions containing one or more peroxyacid to control the growth of pathogens, such as Salmonella , in animal feed. Another aspect of the present invention relates to a formaldehyde-free antimicrobial composition comprising one or more peroxyacid, where the composition is capable of controlling the growth of pathogens, such as Salmonella , in animal feed. Another aspect of the present invention relates to an animal feed additive comprising a buffered peroxyacid composition that is effective at controlling the growth of pathogens while preserving the nutrient content in the animal feed, where the buffered composition is less corrosive to steel and feed mill equipment than non-buffered compositions.

Inventors

  • SALAKLANG, JATUPORN
  • THNG, Agnes Hwee Hong
  • RAVI, SHASHANK
  • CHIRAKKAL, HARIDASAN

Assignees

  • Kemin Industries, Inc.

Dates

Publication Date
20260513
Application Date
20240708

Claims (20)

  1. 1. An animal feed additive comprising one or more peroxyacid in an amount effective to reduce Salmonella present in the animal feed to less than detection level within 24 hours, wherein the peroxyacid is selected from the group consisting of Cl to CIO alkyl chains and wherein the composition does not contain formaldehyde.
  2. 2. The feed additive of Claim 1, wherein the additive comprises two or more peroxyacid selected from the group consisting of propionic acid, peracetic acid, peroctanoic acid, and perdecanoic acid.
  3. 3. The feed additive of Claim 1, wherein the additive comprises a combination of two or more peroxyacid selected from the group consisting of propionic acid, peracetic acid, peroctanoic acid, and perdecanoic acid.
  4. 4. The feed additive of Claim 1, wherein the feed additive is a buffered peroxyacid composition that is effective at a pH ranging from about 1 to 12, specifically about 1 to 6, about 7 to 8, about 8 to 10, or about 11 to 12.
  5. 5. The feed additive of Claim 4, wherein the buffered peroxyacid composition contains mono, di, sodium phosphate, and or trisodium phosphate, ammonium phosphate, sodium hydroxide, or ammonium hydroxide.
  6. 6. The feed additive of Claim 1, wherein the additive further comprises one or more esters.
  7. 7. A method of controlling the growth of pathogens in animal feed comprising adding a buffered peroxyacid composition to animal feed in an amount effective to reduce the presence of the pathogen, wherein the composition does not contain formaldehyde and the composition has a pH of about 7 to 12.
  8. 8. The method of Claim 7, wherein the one or more peroxyacid is selected from the group consisting of Cl to CIO alkyl chains.
  9. 9. The method of Claim 7, wherein the composition contains a combination of two or more peroxyacid selected from the group consisting of propionic acid, peracetic acid, peroctanoic acid, and perdecanoic acid.
  10. 10. The method of Claim 7, wherein the composition further comprises one or more esters.
  11. 11. The method of Claim 7, wherein the pH of the composition was adjusted by adding a buffer selected from the group consisting of sodium phosphate, sodium hydroxide, and ammonium hydroxide.
  12. 12. The method of Claim 7, wherein the buffer is sodium phosphate.
  13. 13. The method of Claim 7, wherein the buffered solution is less corrosive to steel than the non-buffered peroxyacid composition.
  14. 14. A method of reducing the amount of Salmonella present in the animal feed to less than detection level comprising: (a) preparing a peroxyacid composition comprising one or more peroxyacids; (b) preparing a buffered peroxyacid composition by adjusting the pH of the peroxyacid composition to about 7 to 12; and (c) applying the buffered peroxyacid composition to animal feed.
  15. 15. The method of Claim 14, wherein the buffered peroxyacid composition does not contain formaldehyde.
  16. 16. The method of Claim 14, wherein the one or more peroxyacid is selected from the group consisting of Cl to CIO alkyl chains.
  17. 17. The method of Claim 14, wherein the one or more peroxyacids are selected from the group consisting of propionic acid, peracetic acid, peroctanoic acid, and perdecanoic acid, and wherein the composition does not contain formaldehyde.
  18. 18. The method of Claim 14, wherein the composition further comprises one or more esters.
  19. 19. The method of Claim 14, wherein the buffered peroxyacid composition is prepared using a buffer selected from the group consisting of sodium phosphate, sodium hydroxide, and ammonium hydroxide.
  20. 20. The method of Claim 14, wherein the buffered peroxyacid composition is less corrosive to steel than the non-buffered peroxyacid composition.

Description

COMPOSITIONS CONTAINING PEROXYACIDS TO CONTROL PATHOGEN GROWTH IN ANIMAL FEED AND RELATED METHODS CROSS-REFERENCE TO RELATED APPLICATIONS The present application claims the benefit of priority to United States Patent Application No. 63/525,315, filed July 6, 2023, entitled "COMPOSITIONS CONTAINING PEROXYACIDS TO CONTROL PATHOGEN GROWTH IN ANIMAL FEED AND RELATED METHODS,” the entire disclosure of which is incorporated herein by reference. BACKGROUND OF THE INVENTION Formaldehyde has long been used in animal feed to control the spread of Salmonella. The use of formaldehyde in feed has raised concerns on safety and animal performance, however, and the use of formaldehyde is not approved for use in feed in certain regions such as the European Union. Due to the rising concerns associated with formaldehyde, there remains a need for suitable alternatives to formaldehyde that are capable of mitigating against the risk of Salmonella contamination in feed. Peracetic acid, also known as peroxyacetic acid or PAA, has been used as a disinfectant in waste-water treatment (Baumeister, L.J., 2008) (Kitis, M., 2004), and food industries, (Stearns etal., 2022) due to its effectiveness against microbial contaminations. Previous studies have reported the demonstrated effects of the combination of certain organic acids and ester. For instance, a study conducted by Higgins and Brinkhaus (1999) concluded that propionic acid is superior against common mold strains such as Aspergillus spp. and Fusarium spp. as compared to other organic acids such as acetic, sorbic, benzoic, undecylenic and lactic acids. Previous studies have also provided evidence on the effectiveness of medium chain fatty acids (MG FA) and its derivatives against common bacteria such as Salmonella spp., Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Campylobacter jejuni (Jackman et al., 2020), (Skrivanova et al., 2004), (Skrivanova et al., 2005), (Marounek et al., 2003), (Batovska et al., 2009). In addition, Cochrane etal. (2020) and Tran etal. (2021) demonstrated the efficacy of MCFA in mitigating porcine epidemic diarrhea (PED) and African swine fever (ASF) viruses, respectively. Although various studies have been conducted with peroxyacids in other contexts, to the inventors' knowledge, the present invention represents the first time that compositions containing various peroxyacid combinations have been studied for controlling pathogen growth in animal feed and compared against peracetic acid and other common antimicrobial products that are commercially available as feed additives, such as Sal CURB RM E Liquid (Kemin Industries), Sal CURB K2 Liquid (Kemin Industries) and Sal CURB Ba Liquid (Kemin Industries), and a disinfectant, such as Pro-Oxine AH (Bio-Cide International). BRIEF SUMMARY OF THE INVENTION The present invention relates to the use of compositions containing one or more peroxyacid to control the growth or spread of Salmonella in animal feed. The present invention further provides the benefit of providing anti-corrosive properties in comparison to other formulations used in the feed mill industry. Another aspect of the present invention relates to a formaldehyde-free antimicrobial composition suitable for animal feed that is capable of controlling the growth of Salmonella in animal feed. The compositions of the present invention show high efficacy in reducing Salmonella and other pathogens, e.g. viruses, mold, etc. even at high pH (i.e. > 7-12). DETAILED DESCRIPTION OF THE DRAWINGS FIG. 1 is a chart representing a preliminary in vitro efficacy study with peroxy acids against Salmonella. FIG. 2 is a chart representing Salmonella count at high contamination level (~ 5 log cfu/g), over 21 days post treatment period. FIG. 3 is a chart representing Salmonella count at low contamination level (~ 2 log cfu/g), over 21 days post- treatment period. FIG. 4 depicts mild steel when dipped into solution containing 1) 24g PPA + 96g water and 2) 24g PPA + 96g Sodium mono, di, tri- phosphate buffer, 3) 120 g water, 4) 24g PAA + 96g water and 5) 24g PAA + 96g Sodium mono, di, tri- phosphate buffer. Solutions denoting 2 kg/ton PPA dosage/ ton feed. F1G.5 depicts the efficacy of PPA mixtures in combination with acetic acid towards Salmonella in the feed matrix. FIG. 6 depicts Salmonella counts (CFU/g) 1, 2, and 7 days post inoculation from the control and 1.0, 2.0, and 3.0 mg PPA/g feed samples. Feed was inoculated with 1E04 CFU/g feed. Bars represent the mean ± standard deviation. PC = positive control, untreated and inoculated feed. FIG. 7 depicts Salmonella counts (CFU/g) 1, 2, and 7 days post inoculation two (days 8, 9, and 14 of the assay) from control and 1.0, 2.0, and 3.0 mg PPA/g feed samples. Feed was inoculated a second time on day 7 with 1 g of 1E6 CFU/g Salmonella dry inoculum (1E4 CFU/g feed). Bars represent the mean ± standard deviation. PC = positive control, untreated and inoculated feed. FIG. 8 Salmonella counts (CFU/g) 1