EP-4741493-A1 - METHOD FOR CULTURING TISSUE-DERIVED CELLS, AND CULTURE DEVICE CONTAINING TISSUE-DERIVED CELLS

Abstract

The present invention aims to provide a novel method for culturing tissue-derived cells. In the method, skin-derived cells are cultured using a cell culture method comprising the step of co-culturing skin-derived cells and one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells.

Inventors

• KAWASHIMA, IKKO
• BANZAI, Kota
• LU, Mengxue
• SAWADA, Misaki

Assignees

• Integriculture Inc.

Dates

Publication Date

20260513

Application Date

20240704

Claims (17)

1. A cell culture method comprising the step of co-culturing: skin-derived cells; and one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells.
2. A cell culture method comprising the step of culturing skin-derived cells in the presence of a culture supernatant obtained by culturing one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells.
3. The cell culture method according to claim 2, wherein the culture supernatant further includes a culture supernatant obtained by culturing skin-derived cells.
4. The cell culture method according to claim 2 or 3, wherein the culturing is co-culturing.
5. The cell culture method according to any one of claims 1 to 4, wherein the skin-derived cells include epithelial cells and/or dermal cells.
6. The cell culture method according to any one of claims 1 to 4, wherein the intestine-derived cells include one or more types of cells selected from the group consisting of intestinal epithelial cells, CBC cells, Paneth cells, enteroendocrine cells, goblet cells, absorptive epithelial cells, M cells, intestinal parietal cells, connective tissue cells, muscle cells, fibroblasts, vascular endothelial cells, pericytes, and progenitor cells or stem cells thereof.
7. The cell culture method according to any one of claims 1 to 4, wherein the heart-derived cells include one or more types of cells selected from the group consisting of cardiomyocytes, nerve cells, connective tissue cells, fibroblasts, vascular endothelial cells, vascular pericytes, blood cells, and progenitor cells or stem cells thereof.
8. The cell culture method according to any one of claims 1 to 4, wherein the brain-derived cells include one or more types of cells selected from the group consisting of nerve cells, glial cells, pituitary cells, vascular endothelial cells, pericytes, and progenitor cells or stem cells thereof.
9. The cell culture method according to any one of claims 1 to 4, wherein the stomach-derived cells include one or more types of cells selected from the group consisting of gastric chief cells, parietal cells, mucous neck cells, and progenitor cells or stem cells thereof.
10. The cell culture method according to any one of claims 1 to 4, wherein the embryonic membrane-derived cells include one or more types of cells selected from the group consisting of the amnion, serosa, allantois, and yolk sac.
11. The cell culture method according to any one of claims 1 to 4, wherein the kidney-derived cells include one or more types of cells selected from the group consisting of epithelial cells, endothelial cells, mesangial cells, visceral epithelial cells, parietal epithelial cells, connective tissue cells, fibroblasts, vascular endothelial cells, pericytes, and progenitor cells or stem cells thereof.
12. The cell culture method according to any one of claims 1 to 4, wherein the lung-derived cells include one or more types of cells selected from the group consisting of endothelial cells, alveolar epithelial cells, and progenitor cells or stem cells thereof.
13. The cell culture method according to any one of claims 1 to 12, wherein the culturing is performed using a serum-free medium.
14. A culture system in which skin-derived cells and one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells are co-cultured.
15. A culture system in which skin-derived cells are cultured in the presence of a culture supernatant obtained by culturing one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells.
16. The culture system according to claim 15, wherein the culture supernatant further includes a culture supernatant obtained by culturing skin-derived cells.
17. The culture system according to claim 15 or 16, wherein the culturing is co-culturing.

Description

[TECHNICAL FIELD] ==CROSS-REFERENCE TO RELATED APPLICATION(S)== This application claims priority based on Japanese Patent Application No. 2023-111706 filed on July 6, 2023, and the basic application is incorporated herein by reference by citation thereof. == TECHNICAL FIELD == The present invention relates to methods for culturing tissue-derived cells and culture apparatus including tissue-derived cells. [BACKGROUND ART] Conventionally, standard media for culturing tissue-derived cells have been DMEM or RPMI-1640 containing 10% to 20% serum (for example, Scientific Reports volume 12, Article number: 827 (2022)). [SUMMARY OF INVENTION] [PROBLEM TO BE SOLVED BY THE INVENTION] An object of the present invention is to provide novel methods for co-culturing a plurality of types of tissue-derived cells, and novel methods for culturing target cells using culture supernatants of a plurality of types of tissue-derived cells. [MEANS FOR SOLVING THE PROBLEM] [I] One embodiment of the present invention is a cell culture method comprising the step of co-culturing skin-derived cells and one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells. Another embodiment of the present invention is a cell culture method comprising the step of culturing skin-derived cells in the presence of a culture supernatant obtained by culturing one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells. In this method, the culture supernatant may further contain a culture supernatant obtained by culturing skin-derived cells. Further, the culturing may be co-culturing. A further embodiment of the present invention is a culture system in which skin-derived cells and one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells are co-cultured. A further embodiment of the present invention is a culture system in which skin-derived cells are cultured in the presence of a culture supernatant obtained by culturing one or more types of cells selected from the group consisting of intestine-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells. The culture supernatant may further contain a culture supernatant obtained by culturing skin-derived cells. The culturing may be co-culturing. [II] One embodiment of the present invention is a cell culture method comprising the step of co-culturing intestine-derived cells and one or more types of cells selected from the group consisting of skin-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells. Another embodiment of the present invention is a cell culture method comprising the step of culturing intestine-derived cells in the presence of a culture supernatant obtained by culturing one or more types of cells selected from the group consisting of skin-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells. In this method, the culture supernatant may further contain a culture supernatant obtained by culturing intestine-derived cells. Further, the culturing may be co-culturing. A further embodiment of the present invention is a culture system in which intestine-derived cells and one or more types of cells selected from the group consisting of skin-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells are co-cultured. A further embodiment of the present invention is a culture system in which intestine-derived cells are cultured in the presence of a culture supernatant obtained by culturing one or more types of cells selected from the group consisting of skin-derived cells, heart-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived cells, and lung-derived cells. The culture supernatant may further contain a culture supernatant obtained by culturing intestine-derived cells. The culturing may be co-culturing. [III] One embodiment of the present invention is a cell culture method comprising the step of co-culturing heart-derived cells and one or more types of cells selected from the group consisting of skin-derived cells, intestine-derived cells, brain-derived cells, stomach-derived cells, embryonic membrane-derived cells, kidney-derived